Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3542 and GFF3543 are separated by 56 nucleotides GFF3543 and GFF3544 are separated by 21 nucleotides
GFF3542 - Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-), at 294,263 to 295,549
GFF3542
GFF3543 - Electron transfer flavoprotein, alpha subunit, at 295,606 to 296,541
GFF3543
GFF3544 - Electron transfer flavoprotein, beta subunit, at 296,563 to 297,327
GFF3544
Position (kb) 295
296
297 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 294.616 kb on + strand, within GFF3542 at 294.716 kb on - strand, within GFF3542 at 294.839 kb on + strand, within GFF3542 at 294.953 kb on - strand, within GFF3542 at 295.166 kb on + strand, within GFF3542 at 295.246 kb on - strand, within GFF3542 at 295.253 kb on - strand, within GFF3542 at 295.417 kb on + strand, within GFF3542 at 295.485 kb on + strand at 295.506 kb on - strand at 295.525 kb on + strand at 295.554 kb on + strand at 295.607 kb on + strand at 295.647 kb on - strand at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.747 kb on - strand, within GFF3543 at 295.820 kb on + strand, within GFF3543 at 295.903 kb on - strand, within GFF3543 at 296.037 kb on + strand, within GFF3543 at 296.038 kb on - strand, within GFF3543 at 296.039 kb on + strand, within GFF3543 at 296.039 kb on + strand, within GFF3543 at 296.064 kb on + strand, within GFF3543 at 296.272 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.387 kb on - strand, within GFF3543 at 296.500 kb on + strand at 296.899 kb on + strand, within GFF3544 at 297.280 kb on - strand at 297.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 294,616 + GFF3542 0.27 +0.4 294,716 - GFF3542 0.35 -0.4 294,839 + GFF3542 0.45 +0.1 294,953 - GFF3542 0.54 -0.1 295,166 + GFF3542 0.70 +0.2 295,246 - GFF3542 0.76 +0.3 295,253 - GFF3542 0.77 -0.4 295,417 + GFF3542 0.90 -0.1 295,485 + -0.1 295,506 - -0.4 295,525 + +1.1 295,554 + -0.9 295,607 + -0.3 295,647 - -0.2 295,747 - GFF3543 0.15 -1.0 295,747 - GFF3543 0.15 +0.6 295,747 - GFF3543 0.15 -2.2 295,747 - GFF3543 0.15 -0.2 295,820 + GFF3543 0.23 -3.6 295,903 - GFF3543 0.32 -0.0 296,037 + GFF3543 0.46 -0.7 296,038 - GFF3543 0.46 -0.6 296,039 + GFF3543 0.46 -1.0 296,039 + GFF3543 0.46 +0.1 296,064 + GFF3543 0.49 -0.9 296,272 - GFF3543 0.71 -0.2 296,387 - GFF3543 0.83 +0.1 296,387 - GFF3543 0.83 +0.3 296,387 - GFF3543 0.83 -0.7 296,500 + -0.5 296,899 + GFF3544 0.44 -0.4 297,280 - +0.3 297,307 - +0.3
Or see this region's nucleotide sequence