Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3493 and GFF3494 overlap by 4 nucleotides GFF3494 and GFF3495 overlap by 4 nucleotides
GFF3493 - Arginine N-succinyltransferase (EC 2.3.1.109), at 245,464 to 246,498
GFF3493
GFF3494 - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71), at 246,495 to 247,973
GFF3494
GFF3495 - Succinylarginine dihydrolase (EC 3.5.3.23), at 247,970 to 249,313
GFF3495
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1 at 245.635 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.639 kb on + strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.640 kb on - strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.724 kb on + strand, within GFF3493 at 245.728 kb on + strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.729 kb on - strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.771 kb on + strand, within GFF3493 at 245.772 kb on - strand, within GFF3493 at 245.776 kb on - strand, within GFF3493 at 245.792 kb on + strand, within GFF3493 at 245.875 kb on + strand, within GFF3493 at 245.899 kb on - strand, within GFF3493 at 246.097 kb on - strand, within GFF3493 at 246.100 kb on + strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.209 kb on - strand, within GFF3493 at 246.250 kb on - strand, within GFF3493 at 246.508 kb on + strand at 246.575 kb on - strand at 246.575 kb on - strand at 246.719 kb on + strand, within GFF3494 at 246.822 kb on - strand, within GFF3494 at 247.775 kb on + strand, within GFF3494 at 247.916 kb on - strand at 247.933 kb on + strand at 248.011 kb on + strand at 248.011 kb on + strand at 248.022 kb on - strand at 248.365 kb on - strand, within GFF3495 at 248.425 kb on - strand, within GFF3495 at 248.877 kb on - strand, within GFF3495 at 248.884 kb on + strand, within GFF3495 at 248.925 kb on + strand, within GFF3495
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 245,635 + GFF3493 0.17 -0.6 245,639 + GFF3493 0.17 -0.2 245,639 + GFF3493 0.17 -0.3 245,640 - GFF3493 0.17 -0.1 245,640 - GFF3493 0.17 +0.4 245,724 + GFF3493 0.25 -0.6 245,724 + GFF3493 0.25 +0.4 245,728 + GFF3493 0.26 -0.2 245,729 - GFF3493 0.26 -0.2 245,729 - GFF3493 0.26 -0.8 245,729 - GFF3493 0.26 -0.1 245,729 - GFF3493 0.26 -0.2 245,771 + GFF3493 0.30 -0.4 245,771 + GFF3493 0.30 -0.1 245,772 - GFF3493 0.30 -1.3 245,776 - GFF3493 0.30 -0.5 245,792 + GFF3493 0.32 -1.1 245,875 + GFF3493 0.40 +0.1 245,899 - GFF3493 0.42 -1.7 246,097 - GFF3493 0.61 -0.1 246,100 + GFF3493 0.61 -0.1 246,209 - GFF3493 0.72 +0.6 246,209 - GFF3493 0.72 +0.2 246,250 - GFF3493 0.76 -0.4 246,508 + -1.8 246,575 - -0.2 246,575 - -0.9 246,719 + GFF3494 0.15 +0.2 246,822 - GFF3494 0.22 +0.3 247,775 + GFF3494 0.87 +0.8 247,916 - -1.7 247,933 + +0.6 248,011 + +0.2 248,011 + -1.0 248,022 - +1.3 248,365 - GFF3495 0.29 -1.8 248,425 - GFF3495 0.34 -0.6 248,877 - GFF3495 0.67 +1.1 248,884 + GFF3495 0.68 -0.2 248,925 + GFF3495 0.71 -0.6
Or see this region's nucleotide sequence