Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3423

Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3422 and GFF3423 are separated by 447 nucleotidesGFF3423 and GFF3424 are separated by 456 nucleotidesGFF3424 and GFF3425 are separated by 321 nucleotides GFF3422 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 186,437 to 187,687 GFF3422 GFF3423 - FIG00643583: hypothetical protein, at 188,135 to 189,259 GFF3423 GFF3424 - FIG01045472: hypothetical protein, at 189,716 to 189,919 GFF3424 GFF3425 - Probable lipoprotein envF precursor, at 190,241 to 191,029 GFF3425 Position (kb) 188 189 190Strain fitness (log2 ratio) -2 -1 0 1at 187.360 kb on + strand, within GFF3422at 188.147 kb on - strandat 188.366 kb on - strand, within GFF3423at 188.376 kb on - strand, within GFF3423at 188.721 kb on - strand, within GFF3423at 188.760 kb on - strand, within GFF3423at 189.513 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI
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187,360 + GFF3422 0.74 -2.6
188,147 - -0.3
188,366 - GFF3423 0.21 -2.0
188,376 - GFF3423 0.21 +0.7
188,721 - GFF3423 0.52 +0.2
188,760 - GFF3423 0.56 -0.2
189,513 + +0.2

Or see this region's nucleotide sequence