Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3385 and GFF3386 are separated by 323 nucleotides GFF3386 and GFF3387 are separated by 2 nucleotides GFF3387 and GFF3388 are separated by 13 nucleotides
GFF3385 - Putative OMR family iron-siderophore receptor precursor, at 149,939 to 152,113
GFF3385
GFF3386 - YcfF/hinT protein: a purine nucleoside phosphoramidase, at 152,437 to 152,814
GFF3386
GFF3387 - YcfL protein: an outer membrane lipoprotein that is part of a salvage cluster, at 152,817 to 153,191
GFF3387
GFF3388 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 153,205 to 153,843
GFF3388
Position (kb)
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 151.503 kb on + strand, within GFF3385 at 151.514 kb on - strand, within GFF3385 at 151.527 kb on + strand, within GFF3385 at 151.543 kb on + strand, within GFF3385 at 151.585 kb on + strand, within GFF3385 at 151.585 kb on + strand, within GFF3385 at 151.717 kb on + strand, within GFF3385 at 151.731 kb on + strand, within GFF3385 at 151.732 kb on - strand, within GFF3385 at 152.027 kb on - strand at 152.047 kb on + strand at 152.052 kb on - strand at 152.246 kb on + strand at 152.324 kb on - strand at 152.389 kb on + strand at 152.412 kb on + strand at 152.571 kb on + strand, within GFF3386 at 152.718 kb on + strand, within GFF3386 at 152.746 kb on + strand, within GFF3386 at 152.751 kb on + strand, within GFF3386 at 152.812 kb on + strand at 152.813 kb on - strand at 152.816 kb on + strand at 152.816 kb on + strand at 152.817 kb on - strand at 153.048 kb on + strand, within GFF3387 at 153.133 kb on + strand, within GFF3387 at 153.183 kb on + strand at 153.264 kb on + strand at 153.433 kb on + strand, within GFF3388 at 153.433 kb on + strand, within GFF3388 at 153.507 kb on - strand, within GFF3388 at 153.529 kb on - strand, within GFF3388 at 153.576 kb on - strand, within GFF3388 at 153.688 kb on - strand, within GFF3388 at 153.729 kb on - strand, within GFF3388 at 153.734 kb on - strand, within GFF3388 at 153.771 kb on - strand, within GFF3388 at 153.782 kb on + strand at 153.783 kb on - strand at 153.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 151,503 + GFF3385 0.72 -0.8 151,514 - GFF3385 0.72 -1.0 151,527 + GFF3385 0.73 +0.5 151,543 + GFF3385 0.74 -0.2 151,585 + GFF3385 0.76 +0.3 151,585 + GFF3385 0.76 -1.4 151,717 + GFF3385 0.82 -1.8 151,731 + GFF3385 0.82 +0.2 151,732 - GFF3385 0.82 -1.0 152,027 - -0.3 152,047 + +0.3 152,052 - +0.3 152,246 + +0.5 152,324 - -1.8 152,389 + -1.4 152,412 + +0.4 152,571 + GFF3386 0.35 -0.3 152,718 + GFF3386 0.74 +0.4 152,746 + GFF3386 0.82 +0.6 152,751 + GFF3386 0.83 +0.4 152,812 + +0.1 152,813 - +0.7 152,816 + +0.1 152,816 + +0.1 152,817 - +0.5 153,048 + GFF3387 0.62 +0.3 153,133 + GFF3387 0.84 +0.2 153,183 + +0.1 153,264 + -0.1 153,433 + GFF3388 0.36 +0.3 153,433 + GFF3388 0.36 -0.0 153,507 - GFF3388 0.47 -1.5 153,529 - GFF3388 0.51 -0.4 153,576 - GFF3388 0.58 +0.4 153,688 - GFF3388 0.76 +0.2 153,729 - GFF3388 0.82 -2.1 153,734 - GFF3388 0.83 -0.5 153,771 - GFF3388 0.89 -0.2 153,782 + -0.8 153,783 - -1.4 153,807 - -0.3
Or see this region's nucleotide sequence