Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3249 and GFF3250 are separated by 216 nucleotides GFF3250 and GFF3251 overlap by 14 nucleotides GFF3251 and GFF3252 are separated by 18 nucleotides GFF3252 and GFF3253 are separated by 123 nucleotides
GFF3249 - Cell division inhibitor, at 24,240 to 24,749
GFF3249
GFF3250 - DNA transformation protein TfoX, at 24,966 to 25,571
GFF3250
GFF3251 - Putative efflux (PET) family inner membrane protein YccS, at 25,558 to 27,711
GFF3251
GFF3252 - Inner membrane protein YccF, at 27,730 to 28,176
GFF3252
GFF3253 - DNA helicase IV, at 28,300 to 30,354
GFF3253
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 24.580 kb on - strand, within GFF3249 at 24.746 kb on - strand at 24.749 kb on + strand at 24.750 kb on - strand at 24.750 kb on - strand at 25.207 kb on - strand, within GFF3250 at 25.209 kb on + strand, within GFF3250 at 25.266 kb on + strand, within GFF3250 at 25.309 kb on + strand, within GFF3250 at 25.337 kb on + strand, within GFF3250 at 25.392 kb on - strand, within GFF3250 at 25.392 kb on - strand, within GFF3250 at 25.471 kb on - strand, within GFF3250 at 25.499 kb on - strand, within GFF3250 at 25.573 kb on + strand at 25.574 kb on - strand at 25.574 kb on - strand at 25.855 kb on - strand, within GFF3251 at 25.856 kb on + strand, within GFF3251 at 25.863 kb on + strand, within GFF3251 at 25.939 kb on - strand, within GFF3251 at 25.941 kb on + strand, within GFF3251 at 25.941 kb on + strand, within GFF3251 at 26.064 kb on + strand, within GFF3251 at 26.124 kb on + strand, within GFF3251 at 26.125 kb on - strand, within GFF3251 at 26.162 kb on - strand, within GFF3251 at 26.315 kb on - strand, within GFF3251 at 26.420 kb on + strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.424 kb on + strand, within GFF3251 at 26.425 kb on - strand, within GFF3251 at 26.710 kb on + strand, within GFF3251 at 26.738 kb on - strand, within GFF3251 at 26.760 kb on + strand, within GFF3251 at 26.761 kb on - strand, within GFF3251 at 26.856 kb on + strand, within GFF3251 at 27.103 kb on + strand, within GFF3251 at 27.191 kb on + strand, within GFF3251 at 27.263 kb on + strand, within GFF3251 at 27.263 kb on + strand, within GFF3251 at 27.264 kb on - strand, within GFF3251 at 27.269 kb on - strand, within GFF3251 at 27.317 kb on - strand, within GFF3251 at 27.338 kb on + strand, within GFF3251 at 27.428 kb on - strand, within GFF3251 at 27.646 kb on - strand at 27.659 kb on + strand at 27.659 kb on + strand at 27.660 kb on - strand at 27.673 kb on - strand at 27.673 kb on - strand at 27.684 kb on + strand at 27.711 kb on + strand at 28.022 kb on + strand, within GFF3252 at 28.083 kb on + strand, within GFF3252 at 28.093 kb on - strand, within GFF3252 at 28.203 kb on - strand at 28.268 kb on - strand at 28.546 kb on + strand, within GFF3253 at 28.626 kb on + strand, within GFF3253 at 28.626 kb on + strand, within GFF3253
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 24,580 - GFF3249 0.67 +0.2 24,746 - -0.8 24,749 + -0.4 24,750 - -0.7 24,750 - -0.0 25,207 - GFF3250 0.40 -0.8 25,209 + GFF3250 0.40 +0.4 25,266 + GFF3250 0.50 -0.7 25,309 + GFF3250 0.57 -1.5 25,337 + GFF3250 0.61 -2.6 25,392 - GFF3250 0.70 -0.2 25,392 - GFF3250 0.70 +0.2 25,471 - GFF3250 0.83 +0.1 25,499 - GFF3250 0.88 -1.0 25,573 + +1.2 25,574 - -1.5 25,574 - +1.3 25,855 - GFF3251 0.14 +0.4 25,856 + GFF3251 0.14 +0.0 25,863 + GFF3251 0.14 +0.9 25,939 - GFF3251 0.18 +0.7 25,941 + GFF3251 0.18 +0.3 25,941 + GFF3251 0.18 +0.4 26,064 + GFF3251 0.23 -0.0 26,124 + GFF3251 0.26 -0.2 26,125 - GFF3251 0.26 -0.2 26,162 - GFF3251 0.28 +0.2 26,315 - GFF3251 0.35 +0.6 26,420 + GFF3251 0.40 +1.2 26,421 - GFF3251 0.40 +0.9 26,421 - GFF3251 0.40 +0.6 26,421 - GFF3251 0.40 +0.9 26,424 + GFF3251 0.40 -0.0 26,425 - GFF3251 0.40 +0.5 26,710 + GFF3251 0.53 +0.8 26,738 - GFF3251 0.55 +0.7 26,760 + GFF3251 0.56 +0.7 26,761 - GFF3251 0.56 +0.5 26,856 + GFF3251 0.60 +1.4 27,103 + GFF3251 0.72 +0.6 27,191 + GFF3251 0.76 +1.1 27,263 + GFF3251 0.79 +0.7 27,263 + GFF3251 0.79 -1.2 27,264 - GFF3251 0.79 +0.1 27,269 - GFF3251 0.79 -1.4 27,317 - GFF3251 0.82 +0.4 27,338 + GFF3251 0.83 -1.5 27,428 - GFF3251 0.87 -0.1 27,646 - +0.5 27,659 + -0.1 27,659 + -0.5 27,660 - -0.6 27,673 - +0.2 27,673 - +0.1 27,684 + +0.5 27,711 + +0.9 28,022 + GFF3252 0.65 +0.0 28,083 + GFF3252 0.79 +0.4 28,093 - GFF3252 0.81 -0.8 28,203 - +0.1 28,268 - -0.1 28,546 + GFF3253 0.12 +0.1 28,626 + GFF3253 0.16 +0.0 28,626 + GFF3253 0.16 +0.3
Or see this region's nucleotide sequence