Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3065 and GFF3066 are separated by 27 nucleotides GFF3066 and GFF3067 are separated by 112 nucleotides GFF3067 and GFF3068 are separated by 64 nucleotides GFF3068 and GFF3069 are separated by 26 nucleotides
GFF3065 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 73,820 to 74,872
GFF3065
GFF3066 - FIG001341: Probable Fe(2+)-trafficking protein YggX, at 74,900 to 75,175
GFF3066
GFF3067 - Membrane-bound lytic murein transglycosylase C precursor (EC 3.2.1.-), at 75,288 to 76,373
GFF3067
GFF3068 - hypothetical protein, at 76,438 to 76,563
GFF3068
GFF3069 - Nucleoside permease NupG, at 76,590 to 77,846
GFF3069
Position (kb) 75
76
77 Strain fitness (log2 ratio) -2
-1
0
1 at 74.305 kb on + strand, within GFF3065 at 74.359 kb on + strand, within GFF3065 at 74.359 kb on + strand, within GFF3065 at 74.532 kb on + strand, within GFF3065 at 74.540 kb on - strand, within GFF3065 at 74.594 kb on + strand, within GFF3065 at 74.594 kb on + strand, within GFF3065 at 74.595 kb on - strand, within GFF3065 at 74.728 kb on + strand, within GFF3065 at 74.841 kb on + strand at 74.850 kb on + strand at 74.899 kb on + strand at 74.900 kb on - strand at 74.990 kb on + strand, within GFF3066 at 74.991 kb on - strand, within GFF3066 at 75.032 kb on - strand, within GFF3066 at 75.143 kb on - strand, within GFF3066 at 75.217 kb on + strand at 75.453 kb on + strand, within GFF3067 at 75.455 kb on + strand, within GFF3067 at 75.496 kb on - strand, within GFF3067 at 75.496 kb on - strand, within GFF3067 at 75.600 kb on - strand, within GFF3067 at 75.605 kb on + strand, within GFF3067 at 75.606 kb on - strand, within GFF3067 at 75.693 kb on + strand, within GFF3067 at 75.809 kb on + strand, within GFF3067 at 75.852 kb on - strand, within GFF3067 at 75.867 kb on + strand, within GFF3067 at 75.868 kb on - strand, within GFF3067 at 75.897 kb on - strand, within GFF3067 at 75.900 kb on + strand, within GFF3067 at 76.000 kb on + strand, within GFF3067 at 76.001 kb on - strand, within GFF3067 at 76.044 kb on - strand, within GFF3067 at 76.110 kb on + strand, within GFF3067 at 76.127 kb on + strand, within GFF3067 at 76.128 kb on - strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.210 kb on + strand, within GFF3067 at 76.211 kb on - strand, within GFF3067 at 76.291 kb on + strand at 76.291 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.332 kb on + strand at 76.333 kb on - strand at 76.334 kb on + strand at 76.611 kb on - strand at 76.658 kb on + strand at 76.671 kb on + strand at 76.671 kb on + strand at 76.672 kb on - strand at 76.673 kb on + strand at 76.720 kb on - strand, within GFF3069 at 76.804 kb on - strand, within GFF3069 at 76.837 kb on + strand, within GFF3069 at 76.906 kb on - strand, within GFF3069 at 76.908 kb on - strand, within GFF3069 at 76.931 kb on + strand, within GFF3069 at 76.932 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.935 kb on - strand, within GFF3069 at 76.941 kb on + strand, within GFF3069 at 76.970 kb on + strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.019 kb on - strand, within GFF3069 at 77.076 kb on + strand, within GFF3069 at 77.121 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.298 kb on - strand, within GFF3069 at 77.334 kb on - strand, within GFF3069
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 74,305 + GFF3065 0.46 +0.6 74,359 + GFF3065 0.51 +0.1 74,359 + GFF3065 0.51 -1.9 74,532 + GFF3065 0.68 -0.5 74,540 - GFF3065 0.68 +0.1 74,594 + GFF3065 0.74 -0.2 74,594 + GFF3065 0.74 +0.8 74,595 - GFF3065 0.74 -0.0 74,728 + GFF3065 0.86 +1.5 74,841 + +1.0 74,850 + +1.4 74,899 + +1.3 74,900 - -0.8 74,990 + GFF3066 0.33 +0.2 74,991 - GFF3066 0.33 +0.6 75,032 - GFF3066 0.48 +0.4 75,143 - GFF3066 0.88 +0.2 75,217 + -0.4 75,453 + GFF3067 0.15 -0.0 75,455 + GFF3067 0.15 +0.4 75,496 - GFF3067 0.19 -0.9 75,496 - GFF3067 0.19 +0.4 75,600 - GFF3067 0.29 -0.1 75,605 + GFF3067 0.29 +0.9 75,606 - GFF3067 0.29 -0.7 75,693 + GFF3067 0.37 -0.4 75,809 + GFF3067 0.48 +0.3 75,852 - GFF3067 0.52 +0.3 75,867 + GFF3067 0.53 -2.1 75,868 - GFF3067 0.53 +1.0 75,897 - GFF3067 0.56 -0.1 75,900 + GFF3067 0.56 -0.8 76,000 + GFF3067 0.66 -0.7 76,001 - GFF3067 0.66 +1.3 76,044 - GFF3067 0.70 -0.4 76,110 + GFF3067 0.76 +0.6 76,127 + GFF3067 0.77 -0.1 76,128 - GFF3067 0.77 +0.0 76,210 + GFF3067 0.85 -0.1 76,210 + GFF3067 0.85 +0.7 76,210 + GFF3067 0.85 +0.1 76,211 - GFF3067 0.85 -1.3 76,291 + -0.4 76,291 + -1.1 76,332 + +0.4 76,332 + +0.7 76,332 + +0.2 76,332 + +0.2 76,333 - -1.5 76,334 + -0.3 76,611 - +0.6 76,658 + -0.1 76,671 + +0.8 76,671 + +1.5 76,672 - -0.1 76,673 + +0.3 76,720 - GFF3069 0.10 +0.1 76,804 - GFF3069 0.17 +0.3 76,837 + GFF3069 0.20 -1.4 76,906 - GFF3069 0.25 +0.7 76,908 - GFF3069 0.25 +0.5 76,931 + GFF3069 0.27 -0.8 76,932 - GFF3069 0.27 -0.2 76,935 - GFF3069 0.27 -0.5 76,935 - GFF3069 0.27 -0.6 76,935 - GFF3069 0.27 +0.1 76,941 + GFF3069 0.28 -0.2 76,970 + GFF3069 0.30 -0.6 77,019 - GFF3069 0.34 -0.2 77,019 - GFF3069 0.34 +0.4 77,076 + GFF3069 0.39 -0.9 77,121 - GFF3069 0.42 +0.2 77,298 - GFF3069 0.56 -0.6 77,298 - GFF3069 0.56 -0.2 77,334 - GFF3069 0.59 +0.8
Or see this region's nucleotide sequence