Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3021 and GFF3022 are separated by 21 nucleotides GFF3022 and GFF3023 are separated by 373 nucleotides GFF3023 and GFF3024 are separated by 25 nucleotides
GFF3021 - Phosphoglycerate kinase (EC 2.7.2.3), at 35,826 to 36,989
GFF3021
GFF3022 - D-erythrose-4-phosphate dehydrogenase (EC 1.2.1.72), at 37,011 to 38,057
GFF3022
GFF3023 - Predicted regulator of STY3230 transporter operon, at 38,431 to 38,856
GFF3023
GFF3024 - Substrate-specific component STY3230 of queuosine-regulated ECF transporter, at 38,882 to 39,460
GFF3024
Position (kb)
37
38
39 Strain fitness (log2 ratio)
-1
0
1 at 37.224 kb on - strand, within GFF3022 at 37.224 kb on - strand, within GFF3022 at 37.234 kb on - strand, within GFF3022 at 37.393 kb on - strand, within GFF3022 at 37.395 kb on - strand, within GFF3022 at 37.465 kb on - strand, within GFF3022 at 37.566 kb on - strand, within GFF3022 at 37.578 kb on + strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.579 kb on - strand, within GFF3022 at 37.752 kb on + strand, within GFF3022 at 37.752 kb on + strand, within GFF3022 at 37.753 kb on - strand, within GFF3022 at 37.753 kb on - strand, within GFF3022 at 37.761 kb on - strand, within GFF3022 at 38.050 kb on - strand at 38.057 kb on + strand at 38.057 kb on + strand at 38.057 kb on + strand at 38.058 kb on - strand at 38.103 kb on - strand at 38.103 kb on - strand at 38.216 kb on + strand at 38.250 kb on + strand at 38.251 kb on - strand at 38.300 kb on + strand at 38.318 kb on - strand at 38.364 kb on - strand at 38.389 kb on + strand at 38.389 kb on + strand at 38.389 kb on + strand at 38.390 kb on - strand at 38.390 kb on - strand at 38.439 kb on - strand at 38.439 kb on - strand at 38.439 kb on - strand at 38.457 kb on + strand at 38.514 kb on - strand, within GFF3023 at 38.541 kb on - strand, within GFF3023 at 38.730 kb on + strand, within GFF3023 at 38.731 kb on - strand, within GFF3023 at 38.745 kb on + strand, within GFF3023 at 38.842 kb on - strand at 38.882 kb on - strand at 38.932 kb on + strand at 38.944 kb on + strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.945 kb on - strand, within GFF3024 at 38.946 kb on - strand, within GFF3024 at 39.009 kb on + strand, within GFF3024 at 39.013 kb on + strand, within GFF3024
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 37,224 - GFF3022 0.20 +0.2 37,224 - GFF3022 0.20 -0.8 37,234 - GFF3022 0.21 +0.7 37,393 - GFF3022 0.36 +0.4 37,395 - GFF3022 0.37 +0.1 37,465 - GFF3022 0.43 +0.1 37,566 - GFF3022 0.53 -0.1 37,578 + GFF3022 0.54 -0.7 37,579 - GFF3022 0.54 +0.4 37,579 - GFF3022 0.54 +0.5 37,579 - GFF3022 0.54 -0.1 37,579 - GFF3022 0.54 +0.2 37,752 + GFF3022 0.71 +0.3 37,752 + GFF3022 0.71 -0.7 37,753 - GFF3022 0.71 +0.4 37,753 - GFF3022 0.71 -1.3 37,761 - GFF3022 0.72 +0.3 38,050 - -0.3 38,057 + +0.3 38,057 + +0.5 38,057 + -0.6 38,058 - -0.0 38,103 - +0.2 38,103 - +0.5 38,216 + -0.8 38,250 + -0.1 38,251 - +0.3 38,300 + +0.6 38,318 - -0.3 38,364 - +0.6 38,389 + +0.6 38,389 + +0.2 38,389 + +0.2 38,390 - +0.2 38,390 - +0.2 38,439 - +1.2 38,439 - +0.5 38,439 - -0.0 38,457 + +0.1 38,514 - GFF3023 0.19 +0.8 38,541 - GFF3023 0.26 +0.1 38,730 + GFF3023 0.70 +0.1 38,731 - GFF3023 0.70 +0.6 38,745 + GFF3023 0.74 +0.1 38,842 - +0.2 38,882 - -1.0 38,932 + +1.0 38,944 + GFF3024 0.11 +0.4 38,945 - GFF3024 0.11 +0.1 38,945 - GFF3024 0.11 +0.3 38,945 - GFF3024 0.11 +0.9 38,946 - GFF3024 0.11 -0.3 39,009 + GFF3024 0.22 -0.1 39,013 + GFF3024 0.23 -0.1
Or see this region's nucleotide sequence