Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2959 and GFF2960 overlap by 14 nucleotides GFF2960 and GFF2961 are separated by 82 nucleotides GFF2961 and GFF2962 overlap by 4 nucleotides
GFF2959 - Cytosine permease, at 43,371 to 44,567
GFF2959
GFF2960 - Cytosine deaminase (EC 3.5.4.1), at 44,554 to 45,834
GFF2960
GFF2961 - Putative sugar isomerase involved in processing of exogenous sialic acid, at 45,917 to 46,384
GFF2961
GFF2962 - N-acetylmannosamine kinase (EC 2.7.1.60), at 46,381 to 47,256
GFF2962
Position (kb) 44
45
46 Strain fitness (log2 ratio) -1
0
1 at 43.572 kb on + strand, within GFF2959 at 43.572 kb on + strand, within GFF2959 at 43.573 kb on - strand, within GFF2959 at 43.677 kb on - strand, within GFF2959 at 43.700 kb on + strand, within GFF2959 at 44.034 kb on - strand, within GFF2959 at 44.266 kb on - strand, within GFF2959 at 44.330 kb on + strand, within GFF2959 at 44.408 kb on + strand, within GFF2959 at 44.409 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.446 kb on - strand, within GFF2959 at 44.496 kb on + strand at 44.736 kb on + strand, within GFF2960 at 44.737 kb on - strand, within GFF2960 at 44.737 kb on - strand, within GFF2960 at 44.826 kb on + strand, within GFF2960 at 45.128 kb on - strand, within GFF2960 at 45.173 kb on - strand, within GFF2960 at 45.300 kb on + strand, within GFF2960 at 45.549 kb on + strand, within GFF2960 at 45.762 kb on - strand at 45.762 kb on - strand at 46.049 kb on - strand, within GFF2961 at 46.163 kb on + strand, within GFF2961 at 46.166 kb on + strand, within GFF2961 at 46.182 kb on + strand, within GFF2961 at 46.239 kb on - strand, within GFF2961 at 46.368 kb on - strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.384 kb on + strand at 46.385 kb on - strand at 46.385 kb on - strand at 46.421 kb on - strand at 46.467 kb on - strand at 46.762 kb on + strand, within GFF2962
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 43,572 + GFF2959 0.17 +0.3 43,572 + GFF2959 0.17 -0.7 43,573 - GFF2959 0.17 +0.6 43,677 - GFF2959 0.26 +0.5 43,700 + GFF2959 0.27 +0.2 44,034 - GFF2959 0.55 +0.1 44,266 - GFF2959 0.75 +0.2 44,330 + GFF2959 0.80 -0.2 44,408 + GFF2959 0.87 -0.1 44,409 - GFF2959 0.87 -0.2 44,446 - GFF2959 0.90 +0.1 44,446 - GFF2959 0.90 +0.6 44,446 - GFF2959 0.90 +1.1 44,446 - GFF2959 0.90 +0.5 44,496 + -0.0 44,736 + GFF2960 0.14 -1.6 44,737 - GFF2960 0.14 +0.4 44,737 - GFF2960 0.14 -0.9 44,826 + GFF2960 0.21 +0.0 45,128 - GFF2960 0.45 -0.7 45,173 - GFF2960 0.48 +0.5 45,300 + GFF2960 0.58 +0.4 45,549 + GFF2960 0.78 +0.4 45,762 - -1.1 45,762 - -0.2 46,049 - GFF2961 0.28 +1.5 46,163 + GFF2961 0.53 +0.1 46,166 + GFF2961 0.53 +0.3 46,182 + GFF2961 0.57 +0.6 46,239 - GFF2961 0.69 +0.1 46,368 - +0.4 46,384 + +0.4 46,384 + -0.9 46,384 + -0.4 46,384 + +0.3 46,385 - -0.0 46,385 - -1.5 46,421 - +1.5 46,467 - -0.5 46,762 + GFF2962 0.43 -0.7
Or see this region's nucleotide sequence