Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2833 and GFF2834 are separated by 57 nucleotides GFF2834 and GFF2835 are separated by 397 nucleotides GFF2835 and GFF2836 overlap by 4 nucleotides GFF2836 and GFF2837 are separated by 65 nucleotides
GFF2833 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 355,639 to 356,490
GFF2833
GFF2834 - FIG00510464: hypothetical protein, at 356,548 to 357,018
GFF2834
GFF2835 - Putative membrane protein, at 357,416 to 357,982
GFF2835
GFF2836 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 357,979 to 358,788
GFF2836
GFF2837 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 358,854 to 360,599
GFF2837
Position (kb) 357
358 Strain fitness (log2 ratio) -2
-1
0
1 at 356.416 kb on - strand at 356.500 kb on - strand at 356.692 kb on - strand, within GFF2834 at 356.719 kb on - strand, within GFF2834 at 356.724 kb on + strand, within GFF2834 at 356.799 kb on - strand, within GFF2834 at 357.424 kb on + strand at 357.424 kb on + strand at 357.425 kb on - strand at 357.431 kb on - strand at 357.602 kb on - strand, within GFF2835 at 357.691 kb on + strand, within GFF2835 at 357.735 kb on + strand, within GFF2835 at 357.738 kb on + strand, within GFF2835 at 357.807 kb on + strand, within GFF2835 at 357.828 kb on + strand, within GFF2835 at 357.828 kb on + strand, within GFF2835 at 357.828 kb on + strand, within GFF2835 at 357.829 kb on - strand, within GFF2835 at 357.832 kb on + strand, within GFF2835 at 357.864 kb on + strand, within GFF2835 at 358.137 kb on - strand, within GFF2836 at 358.229 kb on - strand, within GFF2836 at 358.311 kb on + strand, within GFF2836 at 358.434 kb on + strand, within GFF2836 at 358.497 kb on - strand, within GFF2836 at 358.564 kb on - strand, within GFF2836 at 358.663 kb on - strand, within GFF2836 at 358.729 kb on + strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.731 kb on + strand at 358.732 kb on - strand at 358.740 kb on + strand at 358.780 kb on - strand at 358.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 356,416 - -1.0 356,500 - -1.4 356,692 - GFF2834 0.31 +0.5 356,719 - GFF2834 0.36 +0.5 356,724 + GFF2834 0.37 -1.5 356,799 - GFF2834 0.53 +0.1 357,424 + +0.2 357,424 + -0.1 357,425 - -1.9 357,431 - +0.4 357,602 - GFF2835 0.33 +0.4 357,691 + GFF2835 0.49 +0.5 357,735 + GFF2835 0.56 +0.8 357,738 + GFF2835 0.57 +0.7 357,807 + GFF2835 0.69 +0.3 357,828 + GFF2835 0.73 -0.8 357,828 + GFF2835 0.73 +0.3 357,828 + GFF2835 0.73 +0.6 357,829 - GFF2835 0.73 +0.1 357,832 + GFF2835 0.73 -0.5 357,864 + GFF2835 0.79 +0.8 358,137 - GFF2836 0.20 +1.3 358,229 - GFF2836 0.31 +0.3 358,311 + GFF2836 0.41 -2.3 358,434 + GFF2836 0.56 +0.2 358,497 - GFF2836 0.64 +0.2 358,564 - GFF2836 0.72 -0.3 358,663 - GFF2836 0.84 +0.7 358,729 + -1.0 358,730 - +0.2 358,730 - -1.0 358,731 + +0.3 358,732 - +0.2 358,740 + +1.1 358,780 - +0.3 358,919 + +0.4
Or see this region's nucleotide sequence