Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1554 and GFF1555 are separated by 515 nucleotides GFF1555 and GFF1556 are separated by 92 nucleotides GFF1556 and GFF1557 are separated by 132 nucleotides
GFF1554 - Putative transcriptional regulator, at 17,777 to 18,673
GFF1554
GFF1555 - hypothetical protein, at 19,189 to 19,302
GFF1555
GFF1556 - HoxN/HupN/NixA family nickel/cobalt transporter, at 19,395 to 20,408
GFF1556
GFF1557 - Tricarboxylate transport sensor protein TctE, at 20,541 to 21,956
GFF1557
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1
2 at 18.529 kb on - strand, within GFF1554 at 19.060 kb on - strand at 19.090 kb on + strand at 19.636 kb on + strand, within GFF1556 at 19.636 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.639 kb on + strand, within GFF1556 at 19.640 kb on - strand, within GFF1556 at 19.646 kb on + strand, within GFF1556 at 19.651 kb on - strand, within GFF1556 at 19.767 kb on + strand, within GFF1556 at 19.778 kb on + strand, within GFF1556 at 19.779 kb on - strand, within GFF1556 at 19.858 kb on + strand, within GFF1556 at 19.859 kb on - strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.087 kb on + strand, within GFF1556 at 20.274 kb on - strand, within GFF1556 at 20.275 kb on + strand, within GFF1556 at 20.351 kb on + strand at 20.394 kb on - strand at 20.394 kb on - strand at 20.402 kb on + strand at 20.403 kb on - strand at 20.559 kb on + strand at 20.560 kb on - strand at 20.578 kb on + strand at 20.579 kb on - strand at 20.579 kb on - strand at 20.827 kb on + strand, within GFF1557 at 20.859 kb on + strand, within GFF1557 at 20.860 kb on - strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.026 kb on + strand, within GFF1557 at 21.270 kb on + strand, within GFF1557 at 21.337 kb on - strand, within GFF1557 at 21.337 kb on - strand, within GFF1557 at 21.363 kb on - strand, within GFF1557
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 18,529 - GFF1554 0.84 -0.9 19,060 - -0.1 19,090 + +0.9 19,636 + GFF1556 0.24 -1.3 19,636 + GFF1556 0.24 -0.8 19,639 + GFF1556 0.24 +0.5 19,639 + GFF1556 0.24 +0.6 19,640 - GFF1556 0.24 +0.5 19,646 + GFF1556 0.25 +1.8 19,651 - GFF1556 0.25 -1.2 19,767 + GFF1556 0.37 -0.7 19,778 + GFF1556 0.38 +0.5 19,779 - GFF1556 0.38 -0.3 19,858 + GFF1556 0.46 -0.3 19,859 - GFF1556 0.46 -0.0 20,087 + GFF1556 0.68 +0.4 20,087 + GFF1556 0.68 +0.2 20,087 + GFF1556 0.68 +0.6 20,274 - GFF1556 0.87 +0.1 20,275 + GFF1556 0.87 +0.5 20,351 + +0.1 20,394 - +0.2 20,394 - +0.3 20,402 + +0.3 20,403 - -1.1 20,559 + -0.1 20,560 - +0.7 20,578 + +0.4 20,579 - +0.0 20,579 - +0.7 20,827 + GFF1557 0.20 +0.4 20,859 + GFF1557 0.22 +0.2 20,860 - GFF1557 0.23 +0.4 21,026 + GFF1557 0.34 -0.8 21,026 + GFF1557 0.34 +0.6 21,026 + GFF1557 0.34 +0.5 21,270 + GFF1557 0.51 +0.3 21,337 - GFF1557 0.56 -0.4 21,337 - GFF1557 0.56 +0.0 21,363 - GFF1557 0.58 +0.7
Or see this region's nucleotide sequence