Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF106 and GFF107 are separated by 106 nucleotides GFF107 and GFF108 are separated by 20 nucleotides GFF108 and GFF109 are separated by 29 nucleotides GFF109 and GFF110 are separated by 9 nucleotides
GFF106 - Predicted chaperone lipoprotein YacC, potentially involved in protein secretion, at 92,760 to 93,107
GFF106
GFF107 - Spermidine synthase (EC 2.5.1.16), at 93,214 to 94,074
GFF107
GFF108 - S-adenosylmethionine decarboxylase proenzyme (EC 4.1.1.50), prokaryotic class 1A, at 94,095 to 94,889
GFF108
GFF109 - DeoR family transcriptional regulator probably related to glycerate, glycolaldehyde metabolism, at 94,919 to 95,686
GFF109
GFF110 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 95,696 to 96,679
GFF110
Position (kb) 94
95 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 93.102 kb on - strand at 93.106 kb on - strand at 93.159 kb on + strand at 93.160 kb on - strand at 93.163 kb on + strand at 93.164 kb on - strand at 93.289 kb on + strand at 93.318 kb on - strand, within GFF107 at 93.318 kb on - strand, within GFF107 at 93.371 kb on + strand, within GFF107 at 93.371 kb on + strand, within GFF107 at 93.401 kb on - strand, within GFF107 at 93.488 kb on + strand, within GFF107 at 93.690 kb on + strand, within GFF107 at 93.701 kb on + strand, within GFF107 at 93.723 kb on + strand, within GFF107 at 93.736 kb on + strand, within GFF107 at 93.747 kb on + strand, within GFF107 at 93.903 kb on + strand, within GFF107 at 93.961 kb on - strand, within GFF107 at 93.961 kb on - strand, within GFF107 at 94.046 kb on - strand at 94.049 kb on + strand at 94.050 kb on - strand at 94.151 kb on + strand at 94.155 kb on + strand at 94.156 kb on - strand at 94.197 kb on + strand, within GFF108 at 94.198 kb on - strand, within GFF108 at 94.206 kb on + strand, within GFF108 at 94.207 kb on - strand, within GFF108 at 94.209 kb on - strand, within GFF108 at 94.221 kb on + strand, within GFF108 at 94.425 kb on - strand, within GFF108 at 94.433 kb on + strand, within GFF108 at 94.445 kb on + strand, within GFF108 at 94.445 kb on + strand, within GFF108 at 94.446 kb on - strand, within GFF108 at 94.446 kb on - strand, within GFF108 at 94.446 kb on - strand, within GFF108 at 94.478 kb on - strand, within GFF108 at 94.545 kb on + strand, within GFF108 at 94.623 kb on - strand, within GFF108 at 94.650 kb on - strand, within GFF108 at 94.650 kb on - strand, within GFF108 at 94.656 kb on - strand, within GFF108 at 94.667 kb on + strand, within GFF108 at 94.709 kb on - strand, within GFF108 at 94.751 kb on + strand, within GFF108 at 94.773 kb on + strand, within GFF108 at 94.773 kb on + strand, within GFF108 at 94.774 kb on - strand, within GFF108 at 95.055 kb on + strand, within GFF109 at 95.055 kb on + strand, within GFF109 at 95.059 kb on + strand, within GFF109 at 95.059 kb on + strand, within GFF109 at 95.179 kb on + strand, within GFF109 at 95.649 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 93,102 - -1.1 93,106 - -1.5 93,159 + +0.1 93,160 - -1.5 93,163 + +1.4 93,164 - +0.7 93,289 + +0.7 93,318 - GFF107 0.12 +0.3 93,318 - GFF107 0.12 +0.1 93,371 + GFF107 0.18 +0.4 93,371 + GFF107 0.18 -0.1 93,401 - GFF107 0.22 +0.0 93,488 + GFF107 0.32 +0.3 93,690 + GFF107 0.55 +0.3 93,701 + GFF107 0.57 +1.0 93,723 + GFF107 0.59 +0.9 93,736 + GFF107 0.61 -0.9 93,747 + GFF107 0.62 +0.3 93,903 + GFF107 0.80 -0.1 93,961 - GFF107 0.87 -0.8 93,961 - GFF107 0.87 +0.5 94,046 - +0.4 94,049 + +0.0 94,050 - +0.3 94,151 + -0.2 94,155 + -0.1 94,156 - +0.3 94,197 + GFF108 0.13 -0.2 94,198 - GFF108 0.13 -0.0 94,206 + GFF108 0.14 -0.6 94,207 - GFF108 0.14 -0.9 94,209 - GFF108 0.14 +0.2 94,221 + GFF108 0.16 +0.3 94,425 - GFF108 0.42 -0.1 94,433 + GFF108 0.43 -0.8 94,445 + GFF108 0.44 -0.6 94,445 + GFF108 0.44 -0.9 94,446 - GFF108 0.44 -1.5 94,446 - GFF108 0.44 -0.6 94,446 - GFF108 0.44 +0.7 94,478 - GFF108 0.48 -0.2 94,545 + GFF108 0.57 +0.1 94,623 - GFF108 0.66 -0.6 94,650 - GFF108 0.70 -1.4 94,650 - GFF108 0.70 +0.0 94,656 - GFF108 0.71 +0.4 94,667 + GFF108 0.72 -0.6 94,709 - GFF108 0.77 +0.2 94,751 + GFF108 0.83 +0.5 94,773 + GFF108 0.85 +0.2 94,773 + GFF108 0.85 -0.2 94,774 - GFF108 0.85 -1.0 95,055 + GFF109 0.18 +0.1 95,055 + GFF109 0.18 -0.5 95,059 + GFF109 0.18 +1.0 95,059 + GFF109 0.18 -4.1 95,179 + GFF109 0.34 +2.3 95,649 + -1.3
Or see this region's nucleotide sequence