Experiment: LB_plus_SM_buffer with 6C2_phage 0.25 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1064 and GFF1065 are separated by 196 nucleotides GFF1065 and GFF1066 are separated by 12 nucleotides GFF1066 and GFF1067 overlap by 1 nucleotides
GFF1064 - Right origin-binding protein, at 246,018 to 246,887
GFF1064
GFF1065 - Conserved uncharacterized protein CreA, at 247,084 to 247,572
GFF1065
GFF1066 - Two-component response regulator CreB, at 247,585 to 248,274
GFF1066
GFF1067 - Two-component response regulator CreC, at 248,274 to 249,698
GFF1067
Position (kb) 247
248
249 Strain fitness (log2 ratio) -1
0
1 at 246.731 kb on - strand, within GFF1064 at 247.191 kb on + strand, within GFF1065 at 247.213 kb on - strand, within GFF1065 at 247.292 kb on + strand, within GFF1065 at 247.462 kb on + strand, within GFF1065 at 247.462 kb on + strand, within GFF1065 at 247.498 kb on + strand, within GFF1065 at 247.645 kb on + strand at 247.885 kb on + strand, within GFF1066 at 247.886 kb on - strand, within GFF1066 at 248.128 kb on - strand, within GFF1066 at 248.202 kb on - strand, within GFF1066 at 248.255 kb on - strand at 248.305 kb on - strand at 248.346 kb on - strand at 248.550 kb on + strand, within GFF1067 at 248.555 kb on + strand, within GFF1067 at 248.555 kb on + strand, within GFF1067 at 248.555 kb on + strand, within GFF1067 at 248.556 kb on - strand, within GFF1067 at 248.559 kb on + strand, within GFF1067 at 248.560 kb on - strand, within GFF1067 at 248.560 kb on - strand, within GFF1067 at 248.623 kb on - strand, within GFF1067 at 248.648 kb on + strand, within GFF1067 at 248.706 kb on - strand, within GFF1067 at 248.718 kb on + strand, within GFF1067 at 248.718 kb on + strand, within GFF1067 at 248.718 kb on + strand, within GFF1067 at 248.718 kb on + strand, within GFF1067 at 248.722 kb on + strand, within GFF1067 at 249.054 kb on + strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.055 kb on - strand, within GFF1067 at 249.163 kb on + strand, within GFF1067 at 249.271 kb on + strand, within GFF1067 at 249.272 kb on - strand, within GFF1067
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 0.25 MOI remove 246,731 - GFF1064 0.82 +0.1 247,191 + GFF1065 0.22 -0.7 247,213 - GFF1065 0.26 +0.3 247,292 + GFF1065 0.43 -1.0 247,462 + GFF1065 0.77 -0.1 247,462 + GFF1065 0.77 +0.1 247,498 + GFF1065 0.85 -0.0 247,645 + -0.8 247,885 + GFF1066 0.43 +0.5 247,886 - GFF1066 0.44 +0.7 248,128 - GFF1066 0.79 +1.0 248,202 - GFF1066 0.89 -0.3 248,255 - +0.5 248,305 - -0.1 248,346 - -0.1 248,550 + GFF1067 0.19 -0.4 248,555 + GFF1067 0.20 +0.1 248,555 + GFF1067 0.20 +0.4 248,555 + GFF1067 0.20 +0.2 248,556 - GFF1067 0.20 +0.1 248,559 + GFF1067 0.20 +0.4 248,560 - GFF1067 0.20 +0.7 248,560 - GFF1067 0.20 +0.3 248,623 - GFF1067 0.24 -0.1 248,648 + GFF1067 0.26 +0.6 248,706 - GFF1067 0.30 -0.1 248,718 + GFF1067 0.31 +1.4 248,718 + GFF1067 0.31 +0.9 248,718 + GFF1067 0.31 -0.4 248,718 + GFF1067 0.31 -0.1 248,722 + GFF1067 0.31 +0.1 249,054 + GFF1067 0.55 -0.9 249,055 - GFF1067 0.55 -1.0 249,055 - GFF1067 0.55 +0.3 249,055 - GFF1067 0.55 -0.3 249,163 + GFF1067 0.62 +0.2 249,271 + GFF1067 0.70 +0.5 249,272 - GFF1067 0.70 -0.9
Or see this region's nucleotide sequence