Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_845 and PfGW456L13_846 are separated by 12 nucleotides PfGW456L13_846 and PfGW456L13_847 overlap by 18 nucleotides PfGW456L13_847 and PfGW456L13_848 overlap by 1 nucleotides PfGW456L13_848 and PfGW456L13_849 are separated by 490 nucleotides PfGW456L13_849 and PfGW456L13_850 are separated by 7 nucleotides
PfGW456L13_845 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 952,260 to 953,507
_845
PfGW456L13_846 - Lipoprotein LppL-related protein, at 953,520 to 953,645
_846
PfGW456L13_847 - hypothetical protein, at 953,628 to 953,906
_847
PfGW456L13_848 - Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly, at 953,906 to 954,238
_848
PfGW456L13_849 - Regulator of nucleoside diphosphate kinase, at 954,729 to 955,139
_849
PfGW456L13_850 - Adenylate cyclase (EC 4.6.1.1), at 955,147 to 957,990
_850
Position (kb) 953
954
955 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 952.963 kb on + strand, within PfGW456L13_845 at 952.963 kb on + strand, within PfGW456L13_845 at 952.963 kb on + strand, within PfGW456L13_845 at 952.963 kb on + strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 952.964 kb on - strand, within PfGW456L13_845 at 953.026 kb on + strand, within PfGW456L13_845 at 953.339 kb on - strand, within PfGW456L13_845 at 953.377 kb on + strand, within PfGW456L13_845 at 953.378 kb on - strand, within PfGW456L13_845 at 953.387 kb on - strand at 953.387 kb on - strand at 953.387 kb on - strand at 953.387 kb on - strand at 953.419 kb on + strand at 953.419 kb on + strand at 953.420 kb on - strand at 953.420 kb on - strand at 953.420 kb on - strand at 953.420 kb on - strand at 953.420 kb on - strand at 953.420 kb on - strand at 953.429 kb on + strand at 953.492 kb on - strand at 953.495 kb on + strand at 953.496 kb on - strand at 953.496 kb on - strand at 953.496 kb on - strand at 953.518 kb on + strand at 953.518 kb on + strand at 953.518 kb on + strand at 953.519 kb on - strand at 953.519 kb on - strand at 953.519 kb on - strand at 953.522 kb on - strand at 953.522 kb on - strand at 953.522 kb on - strand at 953.527 kb on + strand at 953.527 kb on + strand at 953.528 kb on - strand at 953.528 kb on - strand at 953.528 kb on - strand at 953.720 kb on - strand, within PfGW456L13_847 at 953.720 kb on - strand, within PfGW456L13_847 at 953.720 kb on - strand, within PfGW456L13_847 at 953.734 kb on - strand, within PfGW456L13_847 at 953.735 kb on + strand, within PfGW456L13_847 at 953.759 kb on - strand, within PfGW456L13_847 at 953.830 kb on - strand, within PfGW456L13_847 at 953.858 kb on + strand, within PfGW456L13_847 at 953.859 kb on - strand, within PfGW456L13_847 at 954.122 kb on + strand, within PfGW456L13_848 at 954.190 kb on + strand, within PfGW456L13_848 at 954.190 kb on + strand, within PfGW456L13_848 at 954.191 kb on - strand, within PfGW456L13_848 at 954.191 kb on - strand, within PfGW456L13_848 at 954.283 kb on - strand at 954.283 kb on - strand at 954.380 kb on + strand at 954.380 kb on + strand at 954.381 kb on - strand at 954.381 kb on - strand at 954.381 kb on - strand at 954.413 kb on + strand at 954.413 kb on + strand at 954.414 kb on - strand at 954.460 kb on - strand at 954.460 kb on - strand at 954.573 kb on + strand at 954.573 kb on + strand at 954.573 kb on + strand at 954.573 kb on + strand at 954.574 kb on - strand at 954.574 kb on - strand at 954.608 kb on - strand at 954.608 kb on - strand at 954.610 kb on - strand at 954.627 kb on - strand at 954.939 kb on + strand, within PfGW456L13_849 at 954.940 kb on - strand, within PfGW456L13_849 at 954.940 kb on - strand, within PfGW456L13_849 at 954.940 kb on - strand, within PfGW456L13_849 at 955.205 kb on + strand at 955.205 kb on + strand at 955.206 kb on - strand at 955.206 kb on - strand at 955.206 kb on - strand at 955.206 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 952,963 + PfGW456L13_845 0.56 +0.0 952,963 + PfGW456L13_845 0.56 +0.0 952,963 + PfGW456L13_845 0.56 +0.0 952,963 + PfGW456L13_845 0.56 +0.0 952,964 - PfGW456L13_845 0.56 +0.0 952,964 - PfGW456L13_845 0.56 +0.6 952,964 - PfGW456L13_845 0.56 -1.6 952,964 - PfGW456L13_845 0.56 +0.0 952,964 - PfGW456L13_845 0.56 -2.0 952,964 - PfGW456L13_845 0.56 -1.0 952,964 - PfGW456L13_845 0.56 +0.0 952,964 - PfGW456L13_845 0.56 -1.6 953,026 + PfGW456L13_845 0.61 -2.3 953,339 - PfGW456L13_845 0.86 -1.0 953,377 + PfGW456L13_845 0.90 -1.6 953,378 - PfGW456L13_845 0.90 -1.0 953,387 - +0.0 953,387 - +0.0 953,387 - -1.6 953,387 - -1.0 953,419 + -1.0 953,419 + +0.0 953,420 - +1.3 953,420 - +0.0 953,420 - -1.0 953,420 - -2.6 953,420 - +0.0 953,420 - -1.6 953,429 + +0.0 953,492 - -1.0 953,495 + +0.0 953,496 - -1.0 953,496 - -1.0 953,496 - -2.6 953,518 + -1.0 953,518 + +0.0 953,518 + +0.0 953,519 - +0.4 953,519 - -1.3 953,519 - +0.4 953,522 - -0.6 953,522 - -0.5 953,522 - -0.2 953,527 + +0.0 953,527 + -2.3 953,528 - +0.5 953,528 - +0.3 953,528 - -0.4 953,720 - PfGW456L13_847 0.33 -0.2 953,720 - PfGW456L13_847 0.33 -0.8 953,720 - PfGW456L13_847 0.33 -0.1 953,734 - PfGW456L13_847 0.38 +1.8 953,735 + PfGW456L13_847 0.38 -3.1 953,759 - PfGW456L13_847 0.47 +1.0 953,830 - PfGW456L13_847 0.72 -0.2 953,858 + PfGW456L13_847 0.82 +1.1 953,859 - PfGW456L13_847 0.83 -0.4 954,122 + PfGW456L13_848 0.65 +0.2 954,190 + PfGW456L13_848 0.85 -0.1 954,190 + PfGW456L13_848 0.85 -1.1 954,191 - PfGW456L13_848 0.86 -1.6 954,191 - PfGW456L13_848 0.86 -1.3 954,283 - +0.9 954,283 - +0.0 954,380 + -2.0 954,380 + +0.7 954,381 - +0.3 954,381 - +0.2 954,381 - -0.7 954,413 + +0.1 954,413 + +0.1 954,414 - -0.5 954,460 - +0.0 954,460 - +0.7 954,573 + +0.3 954,573 + -0.0 954,573 + -0.2 954,573 + -1.2 954,574 - -0.1 954,574 - +0.6 954,608 - +0.4 954,608 - +0.5 954,610 - +0.2 954,627 - +0.6 954,939 + PfGW456L13_849 0.51 -0.5 954,940 - PfGW456L13_849 0.51 -0.2 954,940 - PfGW456L13_849 0.51 -1.6 954,940 - PfGW456L13_849 0.51 -2.0 955,205 + +0.3 955,205 + +0.2 955,206 - -0.2 955,206 - +2.1 955,206 - -0.4 955,206 - -0.5
Or see this region's nucleotide sequence