Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_787 and PfGW456L13_788 are separated by 81 nucleotides PfGW456L13_788 and PfGW456L13_789 are separated by 24 nucleotides PfGW456L13_789 and PfGW456L13_790 overlap by 36 nucleotides PfGW456L13_790 and PfGW456L13_791 overlap by 14 nucleotides PfGW456L13_791 and PfGW456L13_792 are separated by 18 nucleotides
PfGW456L13_787 - Exodeoxyribonuclease III (EC 3.1.11.2), at 890,986 to 891,765
_787
PfGW456L13_788 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 891,847 to 892,491
_788
PfGW456L13_789 - hypothetical protein, at 892,516 to 892,659
_789
PfGW456L13_790 - hypothetical protein, at 892,624 to 893,007
_790
PfGW456L13_791 - hypothetical protein, at 892,994 to 893,566
_791
PfGW456L13_792 - hypothetical protein, at 893,585 to 893,746
_792
Position (kb)
892
893
894 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 891.735 kb on + strand at 891.793 kb on - strand at 891.793 kb on - strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.812 kb on + strand at 891.816 kb on + strand at 891.816 kb on + strand at 891.831 kb on + strand at 891.944 kb on - strand, within PfGW456L13_788 at 892.506 kb on - strand at 892.506 kb on - strand at 892.506 kb on - strand at 892.506 kb on - strand at 892.599 kb on + strand, within PfGW456L13_789 at 892.599 kb on + strand, within PfGW456L13_789 at 892.600 kb on - strand, within PfGW456L13_789 at 892.600 kb on - strand, within PfGW456L13_789 at 892.600 kb on - strand, within PfGW456L13_789 at 892.600 kb on - strand, within PfGW456L13_789 at 892.708 kb on + strand, within PfGW456L13_790 at 892.708 kb on + strand, within PfGW456L13_790 at 892.708 kb on + strand, within PfGW456L13_790 at 892.709 kb on - strand, within PfGW456L13_790 at 892.709 kb on - strand, within PfGW456L13_790 at 892.715 kb on + strand, within PfGW456L13_790 at 892.716 kb on - strand, within PfGW456L13_790 at 892.797 kb on - strand, within PfGW456L13_790 at 892.797 kb on - strand, within PfGW456L13_790 at 893.169 kb on + strand, within PfGW456L13_791 at 893.169 kb on + strand, within PfGW456L13_791 at 893.169 kb on + strand, within PfGW456L13_791 at 893.170 kb on - strand, within PfGW456L13_791 at 893.312 kb on + strand, within PfGW456L13_791 at 893.346 kb on + strand, within PfGW456L13_791 at 893.346 kb on + strand, within PfGW456L13_791 at 893.346 kb on + strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.347 kb on - strand, within PfGW456L13_791 at 893.361 kb on + strand, within PfGW456L13_791 at 893.361 kb on + strand, within PfGW456L13_791 at 893.361 kb on + strand, within PfGW456L13_791 at 893.361 kb on + strand, within PfGW456L13_791 at 893.362 kb on - strand, within PfGW456L13_791 at 893.430 kb on + strand, within PfGW456L13_791 at 893.430 kb on + strand, within PfGW456L13_791 at 893.430 kb on + strand, within PfGW456L13_791 at 893.430 kb on + strand, within PfGW456L13_791 at 893.431 kb on - strand, within PfGW456L13_791 at 893.431 kb on - strand, within PfGW456L13_791 at 893.431 kb on - strand, within PfGW456L13_791 at 893.453 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.455 kb on + strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.456 kb on - strand, within PfGW456L13_791 at 893.462 kb on + strand, within PfGW456L13_791 at 893.463 kb on - strand, within PfGW456L13_791 at 893.690 kb on + strand, within PfGW456L13_792 at 893.690 kb on + strand, within PfGW456L13_792 at 893.690 kb on + strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.691 kb on - strand, within PfGW456L13_792 at 893.786 kb on + strand at 893.786 kb on + strand at 893.786 kb on + strand at 893.786 kb on + strand at 893.786 kb on + strand at 893.786 kb on + strand at 893.796 kb on + strand at 893.796 kb on + strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.797 kb on - strand at 893.849 kb on + strand at 893.849 kb on + strand at 893.849 kb on + strand at 893.849 kb on + strand at 893.850 kb on - strand at 893.850 kb on - strand at 893.878 kb on + strand at 893.878 kb on + strand at 893.878 kb on + strand at 893.879 kb on - strand at 893.879 kb on - strand at 893.895 kb on + strand at 893.895 kb on + strand at 893.896 kb on - strand at 893.917 kb on - strand at 893.945 kb on + strand at 893.987 kb on + strand at 893.988 kb on - strand at 893.988 kb on - strand at 893.995 kb on + strand at 893.996 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 891,735 + -0.4 891,793 - +0.6 891,793 - +0.0 891,812 + +0.3 891,812 + +0.9 891,812 + +2.1 891,812 + -0.6 891,812 + +0.9 891,812 + +1.3 891,812 + -1.0 891,812 + +1.7 891,816 + +0.2 891,816 + -0.3 891,831 + -0.1 891,944 - PfGW456L13_788 0.15 +0.0 892,506 - -2.1 892,506 - -0.6 892,506 - +0.9 892,506 - -0.0 892,599 + PfGW456L13_789 0.58 +0.9 892,599 + PfGW456L13_789 0.58 -0.7 892,600 - PfGW456L13_789 0.58 -1.0 892,600 - PfGW456L13_789 0.58 +1.3 892,600 - PfGW456L13_789 0.58 +0.3 892,600 - PfGW456L13_789 0.58 -1.6 892,708 + PfGW456L13_790 0.22 -0.6 892,708 + PfGW456L13_790 0.22 +0.4 892,708 + PfGW456L13_790 0.22 +0.6 892,709 - PfGW456L13_790 0.22 +0.8 892,709 - PfGW456L13_790 0.22 +1.1 892,715 + PfGW456L13_790 0.24 +0.5 892,716 - PfGW456L13_790 0.24 +0.2 892,797 - PfGW456L13_790 0.45 -0.2 892,797 - PfGW456L13_790 0.45 +0.9 893,169 + PfGW456L13_791 0.31 -0.9 893,169 + PfGW456L13_791 0.31 -0.6 893,169 + PfGW456L13_791 0.31 +0.1 893,170 - PfGW456L13_791 0.31 +0.5 893,312 + PfGW456L13_791 0.55 -0.2 893,346 + PfGW456L13_791 0.61 +1.0 893,346 + PfGW456L13_791 0.61 +0.6 893,346 + PfGW456L13_791 0.61 -1.4 893,347 - PfGW456L13_791 0.62 +0.1 893,347 - PfGW456L13_791 0.62 -0.8 893,347 - PfGW456L13_791 0.62 -0.8 893,347 - PfGW456L13_791 0.62 -0.3 893,347 - PfGW456L13_791 0.62 +0.0 893,347 - PfGW456L13_791 0.62 -0.4 893,361 + PfGW456L13_791 0.64 +1.2 893,361 + PfGW456L13_791 0.64 -2.0 893,361 + PfGW456L13_791 0.64 +1.3 893,361 + PfGW456L13_791 0.64 +0.3 893,362 - PfGW456L13_791 0.64 -0.3 893,430 + PfGW456L13_791 0.76 -0.4 893,430 + PfGW456L13_791 0.76 -0.7 893,430 + PfGW456L13_791 0.76 +0.1 893,430 + PfGW456L13_791 0.76 +1.2 893,431 - PfGW456L13_791 0.76 +0.4 893,431 - PfGW456L13_791 0.76 -0.6 893,431 - PfGW456L13_791 0.76 -0.2 893,453 + PfGW456L13_791 0.80 -1.5 893,455 + PfGW456L13_791 0.80 -1.0 893,455 + PfGW456L13_791 0.80 +0.8 893,455 + PfGW456L13_791 0.80 -0.4 893,455 + PfGW456L13_791 0.80 +0.6 893,455 + PfGW456L13_791 0.80 +0.3 893,455 + PfGW456L13_791 0.80 -0.3 893,455 + PfGW456L13_791 0.80 +0.9 893,455 + PfGW456L13_791 0.80 -0.2 893,455 + PfGW456L13_791 0.80 +1.3 893,455 + PfGW456L13_791 0.80 -1.0 893,455 + PfGW456L13_791 0.80 -0.5 893,455 + PfGW456L13_791 0.80 +1.0 893,455 + PfGW456L13_791 0.80 +0.0 893,456 - PfGW456L13_791 0.81 +0.7 893,456 - PfGW456L13_791 0.81 -0.6 893,456 - PfGW456L13_791 0.81 -0.2 893,456 - PfGW456L13_791 0.81 +0.3 893,456 - PfGW456L13_791 0.81 -1.2 893,456 - PfGW456L13_791 0.81 +0.5 893,456 - PfGW456L13_791 0.81 +0.7 893,456 - PfGW456L13_791 0.81 +0.3 893,456 - PfGW456L13_791 0.81 +0.4 893,456 - PfGW456L13_791 0.81 +1.2 893,456 - PfGW456L13_791 0.81 +1.4 893,456 - PfGW456L13_791 0.81 -0.7 893,462 + PfGW456L13_791 0.82 +0.0 893,463 - PfGW456L13_791 0.82 +0.4 893,690 + PfGW456L13_792 0.65 +0.2 893,690 + PfGW456L13_792 0.65 +0.1 893,690 + PfGW456L13_792 0.65 +0.3 893,691 - PfGW456L13_792 0.65 +0.6 893,691 - PfGW456L13_792 0.65 -0.2 893,691 - PfGW456L13_792 0.65 -1.3 893,691 - PfGW456L13_792 0.65 +1.6 893,691 - PfGW456L13_792 0.65 -0.0 893,691 - PfGW456L13_792 0.65 +0.3 893,786 + -1.1 893,786 + -0.5 893,786 + -0.1 893,786 + -0.2 893,786 + +1.0 893,786 + -0.2 893,796 + +0.4 893,796 + -0.3 893,797 - +0.0 893,797 - +0.0 893,797 - +1.4 893,797 - -1.4 893,797 - +1.3 893,797 - +0.2 893,797 - -0.4 893,797 - -0.3 893,849 + +1.2 893,849 + -0.1 893,849 + -1.1 893,849 + +1.0 893,850 - +1.5 893,850 - +0.2 893,878 + -0.9 893,878 + +0.7 893,878 + +0.0 893,879 - +0.5 893,879 - +1.0 893,895 + -0.5 893,895 + -1.0 893,896 - +1.6 893,917 - -1.0 893,945 + +2.4 893,987 + +1.0 893,988 - -2.0 893,988 - -0.1 893,995 + +1.0 893,996 - -0.6
Or see this region's nucleotide sequence