Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_70 and PfGW456L13_71 overlap by 4 nucleotides PfGW456L13_71 and PfGW456L13_72 are separated by 396 nucleotides PfGW456L13_72 and PfGW456L13_73 are separated by 155 nucleotides
PfGW456L13_70 - Cobalt-precorrin-6 synthase, anaerobic, at 81,392 to 82,489
_70
PfGW456L13_71 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 82,486 to 83,211
_71
PfGW456L13_72 - hypothetical protein, at 83,608 to 83,931
_72
PfGW456L13_73 - FIG00955840: hypothetical protein, at 84,087 to 84,440
_73
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 81.651 kb on - strand, within PfGW456L13_70 at 81.651 kb on - strand, within PfGW456L13_70 at 81.821 kb on + strand, within PfGW456L13_70 at 81.821 kb on + strand at 81.821 kb on + strand, within PfGW456L13_70 at 81.821 kb on + strand, within PfGW456L13_70 at 81.821 kb on + strand, within PfGW456L13_70 at 81.822 kb on - strand, within PfGW456L13_70 at 81.822 kb on - strand, within PfGW456L13_70 at 81.965 kb on + strand, within PfGW456L13_70 at 81.966 kb on - strand, within PfGW456L13_70 at 82.070 kb on + strand, within PfGW456L13_70 at 82.070 kb on + strand, within PfGW456L13_70 at 82.071 kb on - strand, within PfGW456L13_70 at 82.071 kb on - strand, within PfGW456L13_70 at 82.143 kb on + strand, within PfGW456L13_70 at 82.143 kb on + strand, within PfGW456L13_70 at 82.144 kb on - strand, within PfGW456L13_70 at 82.144 kb on - strand, within PfGW456L13_70 at 82.144 kb on - strand, within PfGW456L13_70 at 82.144 kb on - strand, within PfGW456L13_70 at 82.153 kb on + strand, within PfGW456L13_70 at 82.153 kb on + strand, within PfGW456L13_70 at 82.153 kb on + strand, within PfGW456L13_70 at 82.257 kb on + strand, within PfGW456L13_70 at 82.557 kb on + strand at 82.557 kb on + strand at 82.557 kb on + strand at 82.557 kb on + strand at 82.557 kb on + strand at 82.557 kb on + strand at 82.557 kb on + strand at 82.558 kb on - strand at 82.558 kb on - strand at 82.558 kb on - strand at 82.558 kb on - strand at 82.558 kb on - strand at 82.558 kb on - strand at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.561 kb on + strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.562 kb on - strand, within PfGW456L13_71 at 82.653 kb on + strand, within PfGW456L13_71 at 82.699 kb on + strand, within PfGW456L13_71 at 83.180 kb on + strand at 83.180 kb on + strand at 83.180 kb on + strand at 83.180 kb on + strand at 83.180 kb on + strand at 83.181 kb on - strand at 83.181 kb on - strand at 83.209 kb on + strand at 83.286 kb on + strand at 83.286 kb on + strand at 83.287 kb on - strand at 83.287 kb on - strand at 83.287 kb on - strand at 83.290 kb on + strand at 83.290 kb on + strand at 83.290 kb on + strand at 83.290 kb on + strand at 83.291 kb on - strand at 83.291 kb on - strand at 83.291 kb on - strand at 83.291 kb on - strand at 83.359 kb on + strand at 83.359 kb on + strand at 83.359 kb on + strand at 83.360 kb on - strand at 83.360 kb on - strand at 83.386 kb on + strand at 83.387 kb on - strand at 83.393 kb on + strand at 83.393 kb on + strand at 83.393 kb on + strand at 83.394 kb on - strand at 83.394 kb on - strand at 83.437 kb on + strand at 83.438 kb on - strand at 83.455 kb on - strand at 83.456 kb on + strand at 83.456 kb on + strand at 83.456 kb on + strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.457 kb on - strand at 83.463 kb on + strand at 83.464 kb on - strand at 83.464 kb on - strand at 83.551 kb on + strand at 83.552 kb on - strand at 83.602 kb on - strand at 83.698 kb on - strand, within PfGW456L13_72 at 83.868 kb on - strand, within PfGW456L13_72 at 83.897 kb on + strand, within PfGW456L13_72 at 83.897 kb on + strand, within PfGW456L13_72 at 83.897 kb on + strand, within PfGW456L13_72 at 83.897 kb on + strand, within PfGW456L13_72 at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.903 kb on + strand at 83.904 kb on - strand at 83.904 kb on - strand at 83.904 kb on - strand at 83.904 kb on - strand at 83.904 kb on - strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.909 kb on + strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.910 kb on - strand at 83.976 kb on + strand at 83.976 kb on + strand at 83.980 kb on + strand at 83.981 kb on - strand at 83.998 kb on + strand at 83.998 kb on + strand at 83.999 kb on - strand at 83.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 81,651 - PfGW456L13_70 0.24 +0.2 81,651 - PfGW456L13_70 0.24 -0.1 81,821 + PfGW456L13_70 0.39 +0.6 81,821 + -1.0 81,821 + PfGW456L13_70 0.39 +1.1 81,821 + PfGW456L13_70 0.39 +0.3 81,821 + PfGW456L13_70 0.39 -0.3 81,822 - PfGW456L13_70 0.39 -0.3 81,822 - PfGW456L13_70 0.39 -0.6 81,965 + PfGW456L13_70 0.52 +0.4 81,966 - PfGW456L13_70 0.52 +0.1 82,070 + PfGW456L13_70 0.62 -0.1 82,070 + PfGW456L13_70 0.62 -1.2 82,071 - PfGW456L13_70 0.62 -1.9 82,071 - PfGW456L13_70 0.62 +1.6 82,143 + PfGW456L13_70 0.68 -0.8 82,143 + PfGW456L13_70 0.68 -0.7 82,144 - PfGW456L13_70 0.68 -1.1 82,144 - PfGW456L13_70 0.68 -0.8 82,144 - PfGW456L13_70 0.68 -0.7 82,144 - PfGW456L13_70 0.68 +0.9 82,153 + PfGW456L13_70 0.69 -0.3 82,153 + PfGW456L13_70 0.69 -0.3 82,153 + PfGW456L13_70 0.69 -1.3 82,257 + PfGW456L13_70 0.79 +0.2 82,557 + +0.2 82,557 + +0.4 82,557 + +0.3 82,557 + +0.1 82,557 + +0.9 82,557 + -1.0 82,557 + +0.2 82,558 - +0.5 82,558 - -0.6 82,558 - -0.9 82,558 - +1.2 82,558 - -1.4 82,558 - -0.4 82,561 + PfGW456L13_71 0.10 -0.1 82,561 + PfGW456L13_71 0.10 +0.7 82,561 + PfGW456L13_71 0.10 +0.3 82,561 + PfGW456L13_71 0.10 +0.7 82,561 + PfGW456L13_71 0.10 -0.7 82,561 + PfGW456L13_71 0.10 -0.5 82,561 + PfGW456L13_71 0.10 -0.9 82,561 + PfGW456L13_71 0.10 +1.0 82,561 + PfGW456L13_71 0.10 +0.6 82,561 + PfGW456L13_71 0.10 +0.4 82,561 + PfGW456L13_71 0.10 +2.5 82,562 - PfGW456L13_71 0.10 -0.3 82,562 - PfGW456L13_71 0.10 -1.0 82,562 - PfGW456L13_71 0.10 +0.7 82,562 - PfGW456L13_71 0.10 +0.6 82,562 - PfGW456L13_71 0.10 -2.6 82,562 - PfGW456L13_71 0.10 -1.2 82,653 + PfGW456L13_71 0.23 -0.5 82,699 + PfGW456L13_71 0.29 +0.5 83,180 + +0.5 83,180 + +2.2 83,180 + -0.3 83,180 + +1.7 83,180 + -1.1 83,181 - +0.3 83,181 - -0.8 83,209 + +0.7 83,286 + +0.0 83,286 + +0.6 83,287 - +0.3 83,287 - -0.2 83,287 - -0.1 83,290 + -0.8 83,290 + -1.7 83,290 + +0.3 83,290 + +0.9 83,291 - +0.7 83,291 - -0.5 83,291 - +0.2 83,291 - -1.0 83,359 + +0.8 83,359 + -2.0 83,359 + -2.5 83,360 - +1.5 83,360 - -0.4 83,386 + -0.6 83,387 - +1.2 83,393 + -0.1 83,393 + +0.9 83,393 + -0.3 83,394 - +0.0 83,394 - -1.0 83,437 + +0.9 83,438 - +1.0 83,455 - -1.3 83,456 + +0.5 83,456 + +1.9 83,456 + +0.0 83,457 - +0.7 83,457 - -1.4 83,457 - -1.6 83,457 - +0.0 83,457 - -0.1 83,457 - +0.0 83,457 - +0.8 83,463 + +0.3 83,464 - +1.8 83,464 - -0.2 83,551 + +0.7 83,552 - +0.0 83,602 - +0.0 83,698 - PfGW456L13_72 0.28 +0.0 83,868 - PfGW456L13_72 0.80 +0.0 83,897 + PfGW456L13_72 0.89 +0.4 83,897 + PfGW456L13_72 0.89 -0.3 83,897 + PfGW456L13_72 0.89 +0.7 83,897 + PfGW456L13_72 0.89 +0.9 83,903 + +0.2 83,903 + -0.3 83,903 + +0.7 83,903 + +0.8 83,903 + +0.5 83,903 + -0.7 83,903 + -0.2 83,903 + -0.3 83,904 - +0.3 83,904 - -0.7 83,904 - +0.0 83,904 - +0.6 83,904 - -0.5 83,909 + -0.8 83,909 + +0.4 83,909 + +0.2 83,909 + -0.6 83,909 + -0.1 83,909 + +0.1 83,909 + +0.0 83,909 + +0.3 83,910 - +0.3 83,910 - +0.0 83,910 - +0.0 83,910 - -1.4 83,910 - -0.6 83,910 - +0.4 83,910 - -1.3 83,910 - +0.3 83,910 - +0.0 83,910 - +0.1 83,910 - -1.3 83,910 - +0.7 83,976 + -0.7 83,976 + -2.3 83,980 + +0.4 83,981 - +0.5 83,998 + +0.2 83,998 + +0.4 83,999 - +2.0 83,999 - +0.6
Or see this region's nucleotide sequence