Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_4646 and PfGW456L13_4647 are separated by 19 nucleotides PfGW456L13_4647 and PfGW456L13_4648 are separated by 98 nucleotides PfGW456L13_4648 and PfGW456L13_4649 overlap by 15 nucleotides
PfGW456L13_4646 - Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type, at 5,183,261 to 5,184,976
_4646
PfGW456L13_4647 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 5,184,996 to 5,185,949
_4647
PfGW456L13_4648 - DNA polymerase IV (EC 2.7.7.7), at 5,186,048 to 5,186,935
_4648
PfGW456L13_4649 - hypothetical protein, at 5,186,921 to 5,187,187
_4649
Position (kb) 5184
5185
5186 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5184.977 kb on + strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.985 kb on - strand at 5184.986 kb on + strand at 5184.986 kb on + strand at 5184.986 kb on + strand at 5184.986 kb on + strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5184.987 kb on - strand at 5185.015 kb on + strand at 5185.015 kb on + strand at 5185.016 kb on - strand at 5185.016 kb on - strand at 5185.239 kb on + strand, within PfGW456L13_4647 at 5185.250 kb on + strand, within PfGW456L13_4647 at 5185.250 kb on + strand, within PfGW456L13_4647 at 5185.250 kb on + strand, within PfGW456L13_4647 at 5185.250 kb on + strand, within PfGW456L13_4647 at 5185.251 kb on - strand, within PfGW456L13_4647 at 5185.251 kb on - strand, within PfGW456L13_4647 at 5185.251 kb on - strand, within PfGW456L13_4647 at 5185.389 kb on + strand, within PfGW456L13_4647 at 5185.389 kb on + strand, within PfGW456L13_4647 at 5185.389 kb on + strand, within PfGW456L13_4647 at 5185.390 kb on - strand, within PfGW456L13_4647 at 5185.495 kb on - strand, within PfGW456L13_4647 at 5185.495 kb on - strand, within PfGW456L13_4647 at 5185.675 kb on + strand, within PfGW456L13_4647 at 5185.675 kb on + strand, within PfGW456L13_4647 at 5185.675 kb on + strand, within PfGW456L13_4647 at 5185.675 kb on + strand, within PfGW456L13_4647 at 5185.676 kb on - strand, within PfGW456L13_4647 at 5185.676 kb on - strand, within PfGW456L13_4647 at 5185.676 kb on - strand, within PfGW456L13_4647 at 5185.693 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.725 kb on + strand, within PfGW456L13_4647 at 5185.726 kb on - strand, within PfGW456L13_4647 at 5185.726 kb on - strand, within PfGW456L13_4647 at 5185.746 kb on + strand at 5185.746 kb on + strand, within PfGW456L13_4647 at 5185.746 kb on + strand, within PfGW456L13_4647 at 5185.746 kb on + strand, within PfGW456L13_4647 at 5185.747 kb on - strand, within PfGW456L13_4647 at 5185.947 kb on + strand at 5185.947 kb on + strand at 5185.948 kb on - strand at 5185.973 kb on + strand at 5185.973 kb on + strand at 5185.973 kb on + strand at 5185.973 kb on + strand at 5185.973 kb on + strand at 5185.973 kb on + strand at 5185.974 kb on - strand at 5185.974 kb on - strand at 5185.974 kb on - strand at 5186.049 kb on + strand at 5186.169 kb on + strand, within PfGW456L13_4648 at 5186.169 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.209 kb on + strand, within PfGW456L13_4648 at 5186.210 kb on - strand, within PfGW456L13_4648 at 5186.210 kb on - strand, within PfGW456L13_4648 at 5186.262 kb on + strand, within PfGW456L13_4648 at 5186.262 kb on + strand, within PfGW456L13_4648 at 5186.263 kb on - strand, within PfGW456L13_4648 at 5186.263 kb on - strand, within PfGW456L13_4648 at 5186.359 kb on - strand, within PfGW456L13_4648 at 5186.359 kb on - strand, within PfGW456L13_4648 at 5186.362 kb on + strand at 5186.363 kb on - strand, within PfGW456L13_4648 at 5186.516 kb on - strand, within PfGW456L13_4648 at 5186.788 kb on - strand, within PfGW456L13_4648 at 5186.788 kb on - strand, within PfGW456L13_4648 at 5186.827 kb on - strand, within PfGW456L13_4648 at 5186.866 kb on + strand at 5186.867 kb on - strand at 5186.867 kb on - strand at 5186.867 kb on - strand at 5186.867 kb on - strand at 5186.867 kb on - strand at 5186.868 kb on + strand at 5186.868 kb on + strand at 5186.868 kb on + strand at 5186.869 kb on - strand at 5186.869 kb on - strand at 5186.869 kb on - strand at 5186.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 5,184,977 + -0.7 5,184,985 - +1.1 5,184,985 - +0.8 5,184,985 - +0.4 5,184,985 - -1.2 5,184,985 - +0.2 5,184,985 - -1.8 5,184,985 - -2.2 5,184,986 + -3.1 5,184,986 + -0.0 5,184,986 + +0.0 5,184,986 + +0.2 5,184,987 - -0.2 5,184,987 - -1.1 5,184,987 - +1.2 5,184,987 - -0.8 5,184,987 - -0.3 5,184,987 - -1.3 5,184,987 - +0.3 5,184,987 - -0.8 5,184,987 - -0.7 5,185,015 + -1.3 5,185,015 + +0.7 5,185,016 - -0.7 5,185,016 - +0.7 5,185,239 + PfGW456L13_4647 0.25 -1.1 5,185,250 + PfGW456L13_4647 0.27 -0.9 5,185,250 + PfGW456L13_4647 0.27 -1.2 5,185,250 + PfGW456L13_4647 0.27 -0.7 5,185,250 + PfGW456L13_4647 0.27 +0.0 5,185,251 - PfGW456L13_4647 0.27 +0.7 5,185,251 - PfGW456L13_4647 0.27 -1.7 5,185,251 - PfGW456L13_4647 0.27 +0.7 5,185,389 + PfGW456L13_4647 0.41 -2.2 5,185,389 + PfGW456L13_4647 0.41 -1.3 5,185,389 + PfGW456L13_4647 0.41 -0.3 5,185,390 - PfGW456L13_4647 0.41 -1.4 5,185,495 - PfGW456L13_4647 0.52 +0.3 5,185,495 - PfGW456L13_4647 0.52 +1.7 5,185,675 + PfGW456L13_4647 0.71 -0.1 5,185,675 + PfGW456L13_4647 0.71 -1.1 5,185,675 + PfGW456L13_4647 0.71 -4.4 5,185,675 + PfGW456L13_4647 0.71 -1.6 5,185,676 - PfGW456L13_4647 0.71 +0.8 5,185,676 - PfGW456L13_4647 0.71 -0.4 5,185,676 - PfGW456L13_4647 0.71 -0.7 5,185,693 + PfGW456L13_4647 0.73 -0.8 5,185,725 + PfGW456L13_4647 0.76 -1.6 5,185,725 + PfGW456L13_4647 0.76 -0.7 5,185,725 + PfGW456L13_4647 0.76 -1.1 5,185,725 + PfGW456L13_4647 0.76 -0.9 5,185,725 + PfGW456L13_4647 0.76 -2.5 5,185,725 + PfGW456L13_4647 0.76 -1.2 5,185,726 - PfGW456L13_4647 0.77 -2.7 5,185,726 - PfGW456L13_4647 0.77 +0.9 5,185,746 + -1.1 5,185,746 + PfGW456L13_4647 0.79 -0.2 5,185,746 + PfGW456L13_4647 0.79 -1.9 5,185,746 + PfGW456L13_4647 0.79 -1.1 5,185,747 - PfGW456L13_4647 0.79 -1.1 5,185,947 + +0.4 5,185,947 + +0.0 5,185,948 - +0.8 5,185,973 + -0.1 5,185,973 + -0.1 5,185,973 + -0.7 5,185,973 + -1.4 5,185,973 + +0.5 5,185,973 + +0.3 5,185,974 - -0.5 5,185,974 - -0.1 5,185,974 - -0.8 5,186,049 + +1.9 5,186,169 + PfGW456L13_4648 0.14 +0.5 5,186,169 + PfGW456L13_4648 0.14 -0.4 5,186,209 + PfGW456L13_4648 0.18 -0.1 5,186,209 + PfGW456L13_4648 0.18 +0.4 5,186,209 + PfGW456L13_4648 0.18 +1.4 5,186,209 + PfGW456L13_4648 0.18 -1.1 5,186,209 + PfGW456L13_4648 0.18 -1.5 5,186,209 + PfGW456L13_4648 0.18 +0.3 5,186,209 + PfGW456L13_4648 0.18 -0.8 5,186,209 + PfGW456L13_4648 0.18 +0.1 5,186,209 + PfGW456L13_4648 0.18 -1.8 5,186,209 + PfGW456L13_4648 0.18 -0.7 5,186,209 + PfGW456L13_4648 0.18 -0.7 5,186,209 + PfGW456L13_4648 0.18 -0.2 5,186,210 - PfGW456L13_4648 0.18 -1.7 5,186,210 - PfGW456L13_4648 0.18 +0.0 5,186,262 + PfGW456L13_4648 0.24 +0.4 5,186,262 + PfGW456L13_4648 0.24 -3.8 5,186,263 - PfGW456L13_4648 0.24 +0.2 5,186,263 - PfGW456L13_4648 0.24 -1.2 5,186,359 - PfGW456L13_4648 0.35 +0.6 5,186,359 - PfGW456L13_4648 0.35 -1.9 5,186,362 + -0.1 5,186,363 - PfGW456L13_4648 0.35 -0.1 5,186,516 - PfGW456L13_4648 0.53 -0.0 5,186,788 - PfGW456L13_4648 0.83 -0.1 5,186,788 - PfGW456L13_4648 0.83 -0.1 5,186,827 - PfGW456L13_4648 0.88 -0.6 5,186,866 + +0.0 5,186,867 - +1.3 5,186,867 - -1.9 5,186,867 - +0.3 5,186,867 - +1.1 5,186,867 - +0.6 5,186,868 + -1.0 5,186,868 + -0.7 5,186,868 + +0.9 5,186,869 - -1.0 5,186,869 - -0.8 5,186,869 - -0.5 5,186,869 - -0.8
Or see this region's nucleotide sequence