Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_4643 and PfGW456L13_4644 are separated by 214 nucleotides PfGW456L13_4644 and PfGW456L13_4645 are separated by 44 nucleotides
PfGW456L13_4643 - Peptide chain release factor RF-3, at 5,179,989 to 5,180,945
_4643
PfGW456L13_4644 - methylated-DNA--protein-cysteine methyltransferase-related protein, at 5,181,160 to 5,181,513
_4644
PfGW456L13_4645 - AmpG permease, at 5,181,558 to 5,183,117
_4645
Position (kb) 5181
5182 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5180.210 kb on - strand, within PfGW456L13_4643 at 5180.218 kb on + strand, within PfGW456L13_4643 at 5180.218 kb on + strand, within PfGW456L13_4643 at 5180.218 kb on + strand, within PfGW456L13_4643 at 5180.218 kb on + strand, within PfGW456L13_4643 at 5180.219 kb on - strand, within PfGW456L13_4643 at 5180.219 kb on - strand, within PfGW456L13_4643 at 5180.219 kb on - strand, within PfGW456L13_4643 at 5180.275 kb on + strand, within PfGW456L13_4643 at 5180.275 kb on + strand, within PfGW456L13_4643 at 5180.275 kb on + strand, within PfGW456L13_4643 at 5180.275 kb on + strand, within PfGW456L13_4643 at 5180.399 kb on - strand, within PfGW456L13_4643 at 5180.453 kb on - strand, within PfGW456L13_4643 at 5180.453 kb on - strand, within PfGW456L13_4643 at 5180.506 kb on + strand, within PfGW456L13_4643 at 5180.506 kb on + strand, within PfGW456L13_4643 at 5180.506 kb on + strand, within PfGW456L13_4643 at 5180.506 kb on + strand, within PfGW456L13_4643 at 5180.507 kb on - strand, within PfGW456L13_4643 at 5180.507 kb on - strand, within PfGW456L13_4643 at 5180.507 kb on - strand, within PfGW456L13_4643 at 5180.507 kb on - strand, within PfGW456L13_4643 at 5180.569 kb on + strand, within PfGW456L13_4643 at 5180.570 kb on - strand, within PfGW456L13_4643 at 5180.570 kb on - strand, within PfGW456L13_4643 at 5180.570 kb on - strand, within PfGW456L13_4643 at 5180.600 kb on - strand, within PfGW456L13_4643 at 5180.770 kb on + strand, within PfGW456L13_4643 at 5180.770 kb on + strand, within PfGW456L13_4643 at 5180.770 kb on + strand, within PfGW456L13_4643 at 5180.770 kb on + strand, within PfGW456L13_4643 at 5180.770 kb on + strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.771 kb on - strand, within PfGW456L13_4643 at 5180.851 kb on + strand at 5180.852 kb on - strand at 5180.996 kb on + strand at 5180.996 kb on + strand at 5180.996 kb on + strand at 5180.996 kb on + strand at 5180.997 kb on - strand at 5180.997 kb on - strand at 5180.997 kb on - strand at 5180.997 kb on - strand at 5180.997 kb on - strand at 5181.005 kb on + strand at 5181.005 kb on + strand at 5181.005 kb on + strand at 5181.005 kb on + strand at 5181.005 kb on + strand at 5181.006 kb on - strand at 5181.006 kb on - strand at 5181.044 kb on - strand at 5181.076 kb on + strand at 5181.117 kb on + strand at 5181.117 kb on + strand at 5181.118 kb on - strand at 5181.122 kb on + strand at 5181.122 kb on + strand at 5181.122 kb on + strand at 5181.122 kb on + strand at 5181.123 kb on - strand at 5181.123 kb on - strand at 5181.123 kb on - strand at 5181.131 kb on + strand at 5181.131 kb on + strand at 5181.131 kb on + strand at 5181.131 kb on + strand at 5181.132 kb on - strand at 5181.132 kb on - strand at 5181.132 kb on - strand at 5181.217 kb on + strand, within PfGW456L13_4644 at 5181.217 kb on + strand, within PfGW456L13_4644 at 5181.217 kb on + strand, within PfGW456L13_4644 at 5181.217 kb on + strand, within PfGW456L13_4644 at 5181.218 kb on - strand, within PfGW456L13_4644 at 5181.218 kb on - strand, within PfGW456L13_4644 at 5181.237 kb on - strand, within PfGW456L13_4644 at 5181.237 kb on - strand, within PfGW456L13_4644 at 5181.237 kb on - strand, within PfGW456L13_4644 at 5181.259 kb on + strand, within PfGW456L13_4644 at 5181.382 kb on - strand, within PfGW456L13_4644 at 5181.432 kb on - strand, within PfGW456L13_4644 at 5181.460 kb on - strand, within PfGW456L13_4644 at 5181.460 kb on - strand, within PfGW456L13_4644 at 5181.472 kb on - strand, within PfGW456L13_4644 at 5181.472 kb on - strand, within PfGW456L13_4644 at 5181.497 kb on + strand at 5181.511 kb on + strand at 5181.534 kb on - strand at 5181.566 kb on + strand at 5181.567 kb on - strand at 5181.651 kb on + strand at 5181.651 kb on + strand at 5181.652 kb on - strand at 5181.652 kb on - strand at 5181.652 kb on - strand at 5181.720 kb on + strand, within PfGW456L13_4645 at 5181.721 kb on - strand, within PfGW456L13_4645 at 5181.721 kb on - strand, within PfGW456L13_4645 at 5181.744 kb on + strand, within PfGW456L13_4645 at 5181.744 kb on + strand, within PfGW456L13_4645 at 5181.744 kb on + strand, within PfGW456L13_4645 at 5181.744 kb on + strand, within PfGW456L13_4645 at 5181.744 kb on + strand, within PfGW456L13_4645 at 5181.745 kb on - strand, within PfGW456L13_4645 at 5181.745 kb on - strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.020 kb on + strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.021 kb on - strand, within PfGW456L13_4645 at 5182.107 kb on + strand, within PfGW456L13_4645 at 5182.107 kb on + strand, within PfGW456L13_4645 at 5182.107 kb on + strand, within PfGW456L13_4645 at 5182.108 kb on - strand, within PfGW456L13_4645 at 5182.108 kb on - strand, within PfGW456L13_4645 at 5182.108 kb on - strand, within PfGW456L13_4645 at 5182.108 kb on - strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.134 kb on + strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.135 kb on - strand, within PfGW456L13_4645 at 5182.201 kb on + strand, within PfGW456L13_4645 at 5182.202 kb on - strand, within PfGW456L13_4645 at 5182.276 kb on + strand, within PfGW456L13_4645 at 5182.276 kb on + strand, within PfGW456L13_4645 at 5182.276 kb on + strand, within PfGW456L13_4645 at 5182.276 kb on + strand, within PfGW456L13_4645 at 5182.277 kb on - strand, within PfGW456L13_4645 at 5182.277 kb on - strand, within PfGW456L13_4645 at 5182.277 kb on - strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.314 kb on + strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.315 kb on - strand, within PfGW456L13_4645 at 5182.316 kb on + strand, within PfGW456L13_4645 at 5182.316 kb on + strand, within PfGW456L13_4645 at 5182.316 kb on + strand, within PfGW456L13_4645 at 5182.336 kb on + strand, within PfGW456L13_4645 at 5182.337 kb on - strand, within PfGW456L13_4645 at 5182.337 kb on - strand, within PfGW456L13_4645 at 5182.337 kb on - strand, within PfGW456L13_4645 at 5182.404 kb on + strand, within PfGW456L13_4645 at 5182.404 kb on + strand, within PfGW456L13_4645 at 5182.404 kb on + strand, within PfGW456L13_4645 at 5182.405 kb on - strand, within PfGW456L13_4645 at 5182.405 kb on - strand, within PfGW456L13_4645 at 5182.406 kb on + strand, within PfGW456L13_4645 at 5182.406 kb on + strand, within PfGW456L13_4645 at 5182.406 kb on + strand, within PfGW456L13_4645 at 5182.407 kb on - strand, within PfGW456L13_4645
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 5,180,210 - PfGW456L13_4643 0.23 -1.7 5,180,218 + PfGW456L13_4643 0.24 -1.7 5,180,218 + PfGW456L13_4643 0.24 +0.4 5,180,218 + PfGW456L13_4643 0.24 -1.1 5,180,218 + PfGW456L13_4643 0.24 -2.1 5,180,219 - PfGW456L13_4643 0.24 +0.4 5,180,219 - PfGW456L13_4643 0.24 -0.1 5,180,219 - PfGW456L13_4643 0.24 -1.1 5,180,275 + PfGW456L13_4643 0.30 -0.2 5,180,275 + PfGW456L13_4643 0.30 +0.0 5,180,275 + PfGW456L13_4643 0.30 -0.1 5,180,275 + PfGW456L13_4643 0.30 +0.4 5,180,399 - PfGW456L13_4643 0.43 +0.2 5,180,453 - PfGW456L13_4643 0.48 -0.2 5,180,453 - PfGW456L13_4643 0.48 +1.1 5,180,506 + PfGW456L13_4643 0.54 -0.4 5,180,506 + PfGW456L13_4643 0.54 +0.7 5,180,506 + PfGW456L13_4643 0.54 +1.2 5,180,506 + PfGW456L13_4643 0.54 -1.4 5,180,507 - PfGW456L13_4643 0.54 -0.1 5,180,507 - PfGW456L13_4643 0.54 +0.2 5,180,507 - PfGW456L13_4643 0.54 -1.7 5,180,507 - PfGW456L13_4643 0.54 +1.0 5,180,569 + PfGW456L13_4643 0.61 +1.6 5,180,570 - PfGW456L13_4643 0.61 +0.2 5,180,570 - PfGW456L13_4643 0.61 -0.4 5,180,570 - PfGW456L13_4643 0.61 -0.1 5,180,600 - PfGW456L13_4643 0.64 +0.1 5,180,770 + PfGW456L13_4643 0.82 -0.1 5,180,770 + PfGW456L13_4643 0.82 +0.6 5,180,770 + PfGW456L13_4643 0.82 -0.4 5,180,770 + PfGW456L13_4643 0.82 -1.0 5,180,770 + PfGW456L13_4643 0.82 -0.9 5,180,771 - PfGW456L13_4643 0.82 -0.2 5,180,771 - PfGW456L13_4643 0.82 -1.1 5,180,771 - PfGW456L13_4643 0.82 -1.3 5,180,771 - PfGW456L13_4643 0.82 +0.1 5,180,771 - PfGW456L13_4643 0.82 -0.6 5,180,771 - PfGW456L13_4643 0.82 -1.3 5,180,771 - PfGW456L13_4643 0.82 -0.1 5,180,771 - PfGW456L13_4643 0.82 -1.5 5,180,771 - PfGW456L13_4643 0.82 +0.8 5,180,771 - PfGW456L13_4643 0.82 +1.7 5,180,771 - PfGW456L13_4643 0.82 -0.1 5,180,851 + +0.1 5,180,852 - -0.6 5,180,996 + -1.4 5,180,996 + -0.1 5,180,996 + -0.3 5,180,996 + -1.2 5,180,997 - -0.7 5,180,997 - +1.0 5,180,997 - +1.8 5,180,997 - -1.4 5,180,997 - -0.5 5,181,005 + -0.7 5,181,005 + +0.4 5,181,005 + +0.6 5,181,005 + -0.2 5,181,005 + -0.2 5,181,006 - +1.2 5,181,006 - -0.4 5,181,044 - -0.1 5,181,076 + +0.5 5,181,117 + -0.4 5,181,117 + -0.1 5,181,118 - -0.1 5,181,122 + -0.1 5,181,122 + -1.8 5,181,122 + -0.9 5,181,122 + +0.9 5,181,123 - -0.2 5,181,123 - +0.3 5,181,123 - +0.4 5,181,131 + +0.1 5,181,131 + +0.4 5,181,131 + -0.6 5,181,131 + -0.5 5,181,132 - -0.1 5,181,132 - -0.4 5,181,132 - -1.1 5,181,217 + PfGW456L13_4644 0.16 -2.9 5,181,217 + PfGW456L13_4644 0.16 +1.2 5,181,217 + PfGW456L13_4644 0.16 +0.4 5,181,217 + PfGW456L13_4644 0.16 +1.5 5,181,218 - PfGW456L13_4644 0.16 +0.7 5,181,218 - PfGW456L13_4644 0.16 +0.2 5,181,237 - PfGW456L13_4644 0.22 -0.4 5,181,237 - PfGW456L13_4644 0.22 -0.4 5,181,237 - PfGW456L13_4644 0.22 +0.3 5,181,259 + PfGW456L13_4644 0.28 +0.1 5,181,382 - PfGW456L13_4644 0.63 +0.5 5,181,432 - PfGW456L13_4644 0.77 +0.2 5,181,460 - PfGW456L13_4644 0.85 +0.2 5,181,460 - PfGW456L13_4644 0.85 -1.7 5,181,472 - PfGW456L13_4644 0.88 +0.2 5,181,472 - PfGW456L13_4644 0.88 +0.6 5,181,497 + -0.3 5,181,511 + +0.4 5,181,534 - -4.1 5,181,566 + -3.4 5,181,567 - -1.1 5,181,651 + -1.7 5,181,651 + -2.4 5,181,652 - -1.9 5,181,652 - -2.1 5,181,652 - -2.9 5,181,720 + PfGW456L13_4645 0.10 -3.5 5,181,721 - PfGW456L13_4645 0.10 -2.1 5,181,721 - PfGW456L13_4645 0.10 -0.1 5,181,744 + PfGW456L13_4645 0.12 -1.7 5,181,744 + PfGW456L13_4645 0.12 -2.4 5,181,744 + PfGW456L13_4645 0.12 -1.7 5,181,744 + PfGW456L13_4645 0.12 -1.3 5,181,744 + PfGW456L13_4645 0.12 -1.1 5,181,745 - PfGW456L13_4645 0.12 -1.1 5,181,745 - PfGW456L13_4645 0.12 -1.7 5,182,020 + PfGW456L13_4645 0.30 -2.5 5,182,020 + PfGW456L13_4645 0.30 -1.9 5,182,020 + PfGW456L13_4645 0.30 -0.1 5,182,020 + PfGW456L13_4645 0.30 -2.4 5,182,020 + PfGW456L13_4645 0.30 +0.3 5,182,020 + PfGW456L13_4645 0.30 -1.7 5,182,020 + PfGW456L13_4645 0.30 -2.4 5,182,020 + PfGW456L13_4645 0.30 -1.7 5,182,021 - PfGW456L13_4645 0.30 -2.1 5,182,021 - PfGW456L13_4645 0.30 -1.1 5,182,021 - PfGW456L13_4645 0.30 -0.1 5,182,021 - PfGW456L13_4645 0.30 -2.9 5,182,021 - PfGW456L13_4645 0.30 -1.7 5,182,021 - PfGW456L13_4645 0.30 -3.7 5,182,107 + PfGW456L13_4645 0.35 -1.2 5,182,107 + PfGW456L13_4645 0.35 -1.7 5,182,107 + PfGW456L13_4645 0.35 -0.8 5,182,108 - PfGW456L13_4645 0.35 -1.1 5,182,108 - PfGW456L13_4645 0.35 -1.7 5,182,108 - PfGW456L13_4645 0.35 -1.7 5,182,108 - PfGW456L13_4645 0.35 -0.3 5,182,134 + PfGW456L13_4645 0.37 -3.7 5,182,134 + PfGW456L13_4645 0.37 -1.3 5,182,134 + PfGW456L13_4645 0.37 -2.7 5,182,134 + PfGW456L13_4645 0.37 -1.3 5,182,134 + PfGW456L13_4645 0.37 -1.3 5,182,134 + PfGW456L13_4645 0.37 -2.7 5,182,134 + PfGW456L13_4645 0.37 -1.9 5,182,134 + PfGW456L13_4645 0.37 -1.1 5,182,134 + PfGW456L13_4645 0.37 -2.9 5,182,134 + PfGW456L13_4645 0.37 -1.9 5,182,134 + PfGW456L13_4645 0.37 -2.9 5,182,134 + PfGW456L13_4645 0.37 -2.1 5,182,135 - PfGW456L13_4645 0.37 -1.7 5,182,135 - PfGW456L13_4645 0.37 -3.1 5,182,135 - PfGW456L13_4645 0.37 -1.1 5,182,135 - PfGW456L13_4645 0.37 -2.4 5,182,135 - PfGW456L13_4645 0.37 -3.4 5,182,135 - PfGW456L13_4645 0.37 -0.1 5,182,135 - PfGW456L13_4645 0.37 -1.4 5,182,135 - PfGW456L13_4645 0.37 -2.1 5,182,135 - PfGW456L13_4645 0.37 -3.1 5,182,135 - PfGW456L13_4645 0.37 -4.2 5,182,135 - PfGW456L13_4645 0.37 -2.7 5,182,201 + PfGW456L13_4645 0.41 -2.2 5,182,202 - PfGW456L13_4645 0.41 -2.0 5,182,276 + PfGW456L13_4645 0.46 -2.7 5,182,276 + PfGW456L13_4645 0.46 -1.7 5,182,276 + PfGW456L13_4645 0.46 -2.9 5,182,276 + PfGW456L13_4645 0.46 -1.4 5,182,277 - PfGW456L13_4645 0.46 -1.2 5,182,277 - PfGW456L13_4645 0.46 -1.5 5,182,277 - PfGW456L13_4645 0.46 -0.1 5,182,314 + PfGW456L13_4645 0.48 -2.5 5,182,314 + PfGW456L13_4645 0.48 -0.1 5,182,314 + PfGW456L13_4645 0.48 +1.5 5,182,314 + PfGW456L13_4645 0.48 -0.6 5,182,314 + PfGW456L13_4645 0.48 -2.1 5,182,314 + PfGW456L13_4645 0.48 -1.1 5,182,314 + PfGW456L13_4645 0.48 -1.1 5,182,314 + PfGW456L13_4645 0.48 -2.1 5,182,315 - PfGW456L13_4645 0.49 -2.9 5,182,315 - PfGW456L13_4645 0.49 -2.7 5,182,315 - PfGW456L13_4645 0.49 -0.6 5,182,315 - PfGW456L13_4645 0.49 -3.7 5,182,315 - PfGW456L13_4645 0.49 -1.9 5,182,315 - PfGW456L13_4645 0.49 +0.1 5,182,315 - PfGW456L13_4645 0.49 -2.4 5,182,316 + PfGW456L13_4645 0.49 -1.7 5,182,316 + PfGW456L13_4645 0.49 -0.1 5,182,316 + PfGW456L13_4645 0.49 -1.3 5,182,336 + PfGW456L13_4645 0.50 -1.5 5,182,337 - PfGW456L13_4645 0.50 -3.1 5,182,337 - PfGW456L13_4645 0.50 -1.4 5,182,337 - PfGW456L13_4645 0.50 -4.0 5,182,404 + PfGW456L13_4645 0.54 -1.1 5,182,404 + PfGW456L13_4645 0.54 -1.1 5,182,404 + PfGW456L13_4645 0.54 -3.8 5,182,405 - PfGW456L13_4645 0.54 -1.1 5,182,405 - PfGW456L13_4645 0.54 -2.1 5,182,406 + PfGW456L13_4645 0.54 +0.7 5,182,406 + PfGW456L13_4645 0.54 -1.7 5,182,406 + PfGW456L13_4645 0.54 -0.6 5,182,407 - PfGW456L13_4645 0.54 -2.9
Or see this region's nucleotide sequence