Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_365 and PfGW456L13_366 overlap by 4 nucleotides PfGW456L13_366 and PfGW456L13_367 overlap by 4 nucleotides PfGW456L13_367 and PfGW456L13_5160 are separated by 103 nucleotides PfGW456L13_5160 and PfGW456L13_368 are separated by 321 nucleotides
PfGW456L13_365 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 424,316 to 426,547
_365
PfGW456L13_366 - A/G-specific adenine glycosylase (EC 3.2.2.-), at 426,544 to 427,611
_366
PfGW456L13_367 - FIG001341: Probable Fe(2+)-trafficking protein YggX, at 427,608 to 427,880
_367
PfGW456L13_5160 - tRNA-Phe-GAA, at 427,984 to 428,056
_5160
PfGW456L13_368 - hypothetical protein, at 428,378 to 429,457
_368
Position (kb) 426
427
428 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 425.743 kb on + strand, within PfGW456L13_365 at 425.810 kb on + strand, within PfGW456L13_365 at 425.810 kb on + strand, within PfGW456L13_365 at 425.810 kb on + strand, within PfGW456L13_365 at 425.811 kb on - strand, within PfGW456L13_365 at 426.179 kb on - strand, within PfGW456L13_365 at 426.694 kb on + strand, within PfGW456L13_366 at 426.695 kb on - strand, within PfGW456L13_366 at 426.788 kb on - strand, within PfGW456L13_366 at 426.790 kb on + strand, within PfGW456L13_366 at 426.790 kb on + strand, within PfGW456L13_366 at 426.790 kb on + strand, within PfGW456L13_366 at 426.790 kb on + strand, within PfGW456L13_366 at 426.790 kb on + strand, within PfGW456L13_366 at 426.791 kb on - strand, within PfGW456L13_366 at 427.082 kb on - strand, within PfGW456L13_366 at 427.082 kb on - strand, within PfGW456L13_366 at 427.286 kb on - strand, within PfGW456L13_366 at 427.514 kb on - strand at 427.515 kb on + strand at 427.515 kb on + strand at 427.515 kb on + strand at 427.515 kb on + strand at 427.800 kb on + strand, within PfGW456L13_367 at 427.800 kb on + strand, within PfGW456L13_367 at 427.800 kb on + strand, within PfGW456L13_367 at 427.801 kb on - strand, within PfGW456L13_367 at 427.824 kb on + strand, within PfGW456L13_367 at 427.824 kb on + strand, within PfGW456L13_367 at 427.824 kb on + strand, within PfGW456L13_367 at 427.825 kb on - strand, within PfGW456L13_367 at 427.825 kb on - strand, within PfGW456L13_367 at 427.825 kb on - strand, within PfGW456L13_367 at 427.825 kb on - strand, within PfGW456L13_367 at 427.860 kb on + strand at 427.861 kb on - strand at 427.861 kb on - strand at 427.861 kb on - strand at 428.200 kb on + strand at 428.201 kb on - strand at 428.272 kb on + strand at 428.297 kb on + strand at 428.297 kb on + strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.298 kb on - strand at 428.357 kb on + strand at 428.358 kb on - strand at 428.358 kb on - strand at 428.358 kb on - strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.372 kb on + strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.373 kb on - strand at 428.562 kb on + strand, within PfGW456L13_368 at 428.562 kb on + strand, within PfGW456L13_368 at 428.562 kb on + strand, within PfGW456L13_368 at 428.563 kb on - strand at 428.563 kb on - strand, within PfGW456L13_368 at 428.563 kb on - strand, within PfGW456L13_368 at 428.563 kb on - strand, within PfGW456L13_368 at 428.606 kb on + strand, within PfGW456L13_368 at 428.606 kb on + strand, within PfGW456L13_368 at 428.607 kb on - strand, within PfGW456L13_368 at 428.607 kb on - strand, within PfGW456L13_368
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 425,743 + PfGW456L13_365 0.64 -2.0 425,810 + PfGW456L13_365 0.67 -2.3 425,810 + PfGW456L13_365 0.67 +1.4 425,810 + PfGW456L13_365 0.67 -1.2 425,811 - PfGW456L13_365 0.67 +0.0 426,179 - PfGW456L13_365 0.83 -1.0 426,694 + PfGW456L13_366 0.14 -0.2 426,695 - PfGW456L13_366 0.14 -0.3 426,788 - PfGW456L13_366 0.23 -0.6 426,790 + PfGW456L13_366 0.23 +1.6 426,790 + PfGW456L13_366 0.23 +0.7 426,790 + PfGW456L13_366 0.23 -0.3 426,790 + PfGW456L13_366 0.23 +0.7 426,790 + PfGW456L13_366 0.23 +0.2 426,791 - PfGW456L13_366 0.23 +0.3 427,082 - PfGW456L13_366 0.50 -0.4 427,082 - PfGW456L13_366 0.50 +0.6 427,286 - PfGW456L13_366 0.69 -0.3 427,514 - +2.3 427,515 + -0.1 427,515 + +1.4 427,515 + +0.2 427,515 + +0.2 427,800 + PfGW456L13_367 0.70 +0.0 427,800 + PfGW456L13_367 0.70 -3.2 427,800 + PfGW456L13_367 0.70 -0.6 427,801 - PfGW456L13_367 0.71 +0.5 427,824 + PfGW456L13_367 0.79 -2.8 427,824 + PfGW456L13_367 0.79 -0.8 427,824 + PfGW456L13_367 0.79 -3.2 427,825 - PfGW456L13_367 0.79 +0.6 427,825 - PfGW456L13_367 0.79 -1.0 427,825 - PfGW456L13_367 0.79 +0.0 427,825 - PfGW456L13_367 0.79 -0.5 427,860 + +0.8 427,861 - -1.4 427,861 - -2.0 427,861 - -2.6 428,200 + +0.7 428,201 - +0.0 428,272 + +0.2 428,297 + -0.4 428,297 + +0.0 428,298 - +0.3 428,298 - -0.3 428,298 - +0.2 428,298 - -0.8 428,298 - -0.2 428,298 - -3.2 428,298 - -0.4 428,298 - -1.8 428,357 + -0.3 428,358 - -1.6 428,358 - +1.7 428,358 - -0.4 428,372 + -1.1 428,372 + -0.3 428,372 + +0.7 428,372 + +1.9 428,372 + +0.2 428,372 + +0.7 428,372 + -1.3 428,372 + +1.0 428,372 + -0.2 428,372 + -1.4 428,372 + +0.4 428,372 + -0.7 428,372 + -1.1 428,373 - +2.0 428,373 - -0.7 428,373 - -0.6 428,373 - +0.6 428,373 - -1.6 428,373 - +0.4 428,373 - -2.2 428,373 - -0.3 428,373 - -3.3 428,373 - +1.0 428,373 - -1.5 428,373 - +0.7 428,373 - +0.0 428,373 - -1.3 428,373 - -0.1 428,373 - +0.5 428,373 - +0.1 428,373 - +0.1 428,562 + PfGW456L13_368 0.17 -2.3 428,562 + PfGW456L13_368 0.17 -0.8 428,562 + PfGW456L13_368 0.17 +0.6 428,563 - -1.0 428,563 - PfGW456L13_368 0.17 +0.4 428,563 - PfGW456L13_368 0.17 -0.4 428,563 - PfGW456L13_368 0.17 -0.2 428,606 + PfGW456L13_368 0.21 +0.4 428,606 + PfGW456L13_368 0.21 +1.5 428,607 - PfGW456L13_368 0.21 -0.1 428,607 - PfGW456L13_368 0.21 +0.2
Or see this region's nucleotide sequence