Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2953 and PfGW456L13_2954 are separated by 182 nucleotides PfGW456L13_2954 and PfGW456L13_2955 are separated by 14 nucleotides
PfGW456L13_2953 - Probable transmembrane protein, at 3,294,258 to 3,294,899
_2953
PfGW456L13_2954 - RND efflux system, inner membrane transporter CmeB, at 3,295,082 to 3,298,180
_2954
PfGW456L13_2955 - RND efflux system, membrane fusion protein CmeA, at 3,298,195 to 3,299,352
_2955
Position (kb)
3295
3296
3297
3298
3299 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3294.214 kb on + strand at 3294.214 kb on + strand at 3294.239 kb on + strand at 3294.239 kb on + strand at 3294.240 kb on - strand at 3294.240 kb on - strand at 3294.281 kb on + strand at 3294.282 kb on - strand at 3294.418 kb on + strand, within PfGW456L13_2953 at 3294.419 kb on - strand, within PfGW456L13_2953 at 3294.433 kb on + strand, within PfGW456L13_2953 at 3294.433 kb on + strand, within PfGW456L13_2953 at 3294.434 kb on - strand, within PfGW456L13_2953 at 3294.434 kb on - strand, within PfGW456L13_2953 at 3294.494 kb on - strand, within PfGW456L13_2953 at 3294.494 kb on - strand, within PfGW456L13_2953 at 3294.766 kb on + strand, within PfGW456L13_2953 at 3294.766 kb on + strand, within PfGW456L13_2953 at 3294.766 kb on + strand, within PfGW456L13_2953 at 3294.766 kb on + strand, within PfGW456L13_2953 at 3294.767 kb on - strand, within PfGW456L13_2953 at 3294.920 kb on + strand at 3294.920 kb on + strand at 3294.921 kb on - strand at 3295.031 kb on + strand at 3295.032 kb on - strand at 3295.032 kb on - strand at 3295.032 kb on - strand at 3295.032 kb on - strand at 3295.066 kb on + strand at 3295.083 kb on + strand at 3295.083 kb on + strand at 3295.083 kb on + strand at 3295.083 kb on + strand at 3295.083 kb on + strand at 3295.083 kb on + strand at 3295.084 kb on - strand at 3295.084 kb on - strand at 3295.084 kb on - strand at 3295.084 kb on - strand at 3295.341 kb on + strand at 3295.342 kb on - strand at 3295.784 kb on + strand, within PfGW456L13_2954 at 3295.784 kb on + strand, within PfGW456L13_2954 at 3295.926 kb on + strand, within PfGW456L13_2954 at 3295.926 kb on + strand, within PfGW456L13_2954 at 3295.926 kb on + strand, within PfGW456L13_2954 at 3295.926 kb on + strand, within PfGW456L13_2954 at 3295.927 kb on - strand, within PfGW456L13_2954 at 3295.927 kb on - strand, within PfGW456L13_2954 at 3296.091 kb on - strand, within PfGW456L13_2954 at 3296.170 kb on + strand, within PfGW456L13_2954 at 3296.171 kb on - strand, within PfGW456L13_2954 at 3296.171 kb on - strand, within PfGW456L13_2954 at 3296.171 kb on - strand, within PfGW456L13_2954 at 3296.475 kb on + strand, within PfGW456L13_2954 at 3296.475 kb on + strand, within PfGW456L13_2954 at 3296.476 kb on - strand, within PfGW456L13_2954 at 3296.590 kb on - strand, within PfGW456L13_2954 at 3296.784 kb on + strand, within PfGW456L13_2954 at 3297.093 kb on + strand, within PfGW456L13_2954 at 3297.159 kb on + strand, within PfGW456L13_2954 at 3297.159 kb on + strand, within PfGW456L13_2954 at 3297.159 kb on + strand, within PfGW456L13_2954 at 3297.160 kb on - strand, within PfGW456L13_2954 at 3297.160 kb on - strand, within PfGW456L13_2954 at 3297.160 kb on - strand, within PfGW456L13_2954 at 3297.204 kb on + strand, within PfGW456L13_2954 at 3297.204 kb on + strand, within PfGW456L13_2954 at 3297.205 kb on - strand, within PfGW456L13_2954 at 3297.205 kb on - strand, within PfGW456L13_2954 at 3297.205 kb on - strand, within PfGW456L13_2954 at 3297.216 kb on + strand, within PfGW456L13_2954 at 3297.238 kb on - strand, within PfGW456L13_2954 at 3297.361 kb on - strand, within PfGW456L13_2954 at 3297.652 kb on - strand, within PfGW456L13_2954 at 3297.652 kb on - strand, within PfGW456L13_2954 at 3297.951 kb on + strand at 3297.952 kb on - strand at 3298.035 kb on + strand at 3298.181 kb on - strand at 3298.196 kb on + strand at 3298.477 kb on + strand, within PfGW456L13_2955 at 3298.477 kb on + strand, within PfGW456L13_2955 at 3298.478 kb on - strand, within PfGW456L13_2955 at 3298.848 kb on - strand, within PfGW456L13_2955 at 3298.938 kb on - strand, within PfGW456L13_2955
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 3,294,214 + -0.1 3,294,214 + +0.3 3,294,239 + -0.3 3,294,239 + +0.5 3,294,240 - -0.1 3,294,240 - -0.1 3,294,281 + -0.1 3,294,282 - +0.8 3,294,418 + PfGW456L13_2953 0.25 -2.9 3,294,419 - PfGW456L13_2953 0.25 -0.3 3,294,433 + PfGW456L13_2953 0.27 -0.1 3,294,433 + PfGW456L13_2953 0.27 -0.5 3,294,434 - PfGW456L13_2953 0.27 -1.7 3,294,434 - PfGW456L13_2953 0.27 -0.1 3,294,494 - PfGW456L13_2953 0.37 -0.4 3,294,494 - PfGW456L13_2953 0.37 -1.4 3,294,766 + PfGW456L13_2953 0.79 +0.2 3,294,766 + PfGW456L13_2953 0.79 +1.6 3,294,766 + PfGW456L13_2953 0.79 +0.0 3,294,766 + PfGW456L13_2953 0.79 -1.1 3,294,767 - PfGW456L13_2953 0.79 -0.7 3,294,920 + -0.1 3,294,920 + +1.6 3,294,921 - +1.3 3,295,031 + -1.7 3,295,032 - -1.6 3,295,032 - +0.1 3,295,032 - +1.9 3,295,032 - +0.3 3,295,066 + +0.9 3,295,083 + +1.1 3,295,083 + -1.1 3,295,083 + -1.3 3,295,083 + -0.3 3,295,083 + -0.4 3,295,083 + -1.4 3,295,084 - -2.1 3,295,084 - +0.8 3,295,084 - -0.6 3,295,084 - +0.2 3,295,341 + -1.1 3,295,342 - -2.4 3,295,784 + PfGW456L13_2954 0.23 -1.1 3,295,784 + PfGW456L13_2954 0.23 -2.4 3,295,926 + PfGW456L13_2954 0.27 +1.5 3,295,926 + PfGW456L13_2954 0.27 -3.3 3,295,926 + PfGW456L13_2954 0.27 -1.1 3,295,926 + PfGW456L13_2954 0.27 -1.1 3,295,927 - PfGW456L13_2954 0.27 -1.3 3,295,927 - PfGW456L13_2954 0.27 -2.4 3,296,091 - PfGW456L13_2954 0.33 -2.3 3,296,170 + PfGW456L13_2954 0.35 -2.4 3,296,171 - PfGW456L13_2954 0.35 -2.5 3,296,171 - PfGW456L13_2954 0.35 -1.7 3,296,171 - PfGW456L13_2954 0.35 -2.0 3,296,475 + PfGW456L13_2954 0.45 -2.4 3,296,475 + PfGW456L13_2954 0.45 -1.1 3,296,476 - PfGW456L13_2954 0.45 -1.1 3,296,590 - PfGW456L13_2954 0.49 -1.1 3,296,784 + PfGW456L13_2954 0.55 -2.1 3,297,093 + PfGW456L13_2954 0.65 -1.7 3,297,159 + PfGW456L13_2954 0.67 -1.1 3,297,159 + PfGW456L13_2954 0.67 -1.1 3,297,159 + PfGW456L13_2954 0.67 -3.3 3,297,160 - PfGW456L13_2954 0.67 -3.7 3,297,160 - PfGW456L13_2954 0.67 -0.7 3,297,160 - PfGW456L13_2954 0.67 -0.4 3,297,204 + PfGW456L13_2954 0.68 -1.2 3,297,204 + PfGW456L13_2954 0.68 +1.5 3,297,205 - PfGW456L13_2954 0.69 -2.4 3,297,205 - PfGW456L13_2954 0.69 -2.4 3,297,205 - PfGW456L13_2954 0.69 -1.7 3,297,216 + PfGW456L13_2954 0.69 -2.7 3,297,238 - PfGW456L13_2954 0.70 -1.7 3,297,361 - PfGW456L13_2954 0.74 -1.4 3,297,652 - PfGW456L13_2954 0.83 -0.1 3,297,652 - PfGW456L13_2954 0.83 -3.4 3,297,951 + -0.1 3,297,952 - -0.6 3,298,035 + -1.9 3,298,181 - -0.9 3,298,196 + +0.5 3,298,477 + PfGW456L13_2955 0.24 -0.1 3,298,477 + PfGW456L13_2955 0.24 -2.1 3,298,478 - PfGW456L13_2955 0.24 -1.4 3,298,848 - PfGW456L13_2955 0.56 -1.9 3,298,938 - PfGW456L13_2955 0.64 -2.4
Or see this region's nucleotide sequence