Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2662 and PfGW456L13_2663 are separated by 170 nucleotides PfGW456L13_2663 and PfGW456L13_2664 are separated by 25 nucleotides PfGW456L13_2664 and PfGW456L13_2665 are separated by 19 nucleotides PfGW456L13_2665 and PfGW456L13_2666 are separated by 148 nucleotides PfGW456L13_2666 and PfGW456L13_2667 are separated by 131 nucleotides
PfGW456L13_2662 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 2,963,038 to 2,963,460
_2662
PfGW456L13_2663 - Membrane protein, putative, at 2,963,631 to 2,964,335
_2663
PfGW456L13_2664 - FIG00957702: hypothetical protein, at 2,964,361 to 2,964,690
_2664
PfGW456L13_2665 - hypothetical protein, at 2,964,710 to 2,964,958
_2665
PfGW456L13_2666 - hypothetical protein, at 2,965,107 to 2,965,457
_2666
PfGW456L13_2667 - probable acetyltransferase, at 2,965,589 to 2,966,014
_2667
Position (kb)
2964
2965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2963.463 kb on + strand at 2963.463 kb on + strand at 2963.642 kb on + strand at 2963.642 kb on + strand at 2963.642 kb on + strand at 2963.642 kb on + strand at 2963.643 kb on - strand at 2963.643 kb on - strand at 2963.643 kb on - strand at 2963.644 kb on + strand at 2963.645 kb on - strand at 2963.645 kb on - strand at 2963.878 kb on + strand, within PfGW456L13_2663 at 2963.878 kb on + strand, within PfGW456L13_2663 at 2963.878 kb on + strand, within PfGW456L13_2663 at 2963.878 kb on + strand, within PfGW456L13_2663 at 2963.878 kb on + strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.879 kb on - strand, within PfGW456L13_2663 at 2963.904 kb on + strand, within PfGW456L13_2663 at 2963.904 kb on + strand, within PfGW456L13_2663 at 2963.905 kb on - strand, within PfGW456L13_2663 at 2963.977 kb on + strand, within PfGW456L13_2663 at 2964.021 kb on + strand, within PfGW456L13_2663 at 2964.021 kb on + strand, within PfGW456L13_2663 at 2964.022 kb on - strand, within PfGW456L13_2663 at 2964.022 kb on - strand, within PfGW456L13_2663 at 2964.138 kb on - strand, within PfGW456L13_2663 at 2964.164 kb on + strand, within PfGW456L13_2663 at 2964.164 kb on + strand, within PfGW456L13_2663 at 2964.164 kb on + strand, within PfGW456L13_2663 at 2964.165 kb on - strand, within PfGW456L13_2663 at 2964.264 kb on + strand, within PfGW456L13_2663 at 2964.265 kb on - strand at 2964.265 kb on - strand at 2964.265 kb on - strand at 2964.371 kb on - strand at 2964.390 kb on - strand at 2964.455 kb on + strand, within PfGW456L13_2664 at 2964.456 kb on - strand, within PfGW456L13_2664 at 2964.478 kb on + strand, within PfGW456L13_2664 at 2964.479 kb on - strand, within PfGW456L13_2664 at 2964.479 kb on - strand, within PfGW456L13_2664 at 2964.479 kb on - strand, within PfGW456L13_2664 at 2964.711 kb on + strand at 2964.777 kb on + strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.778 kb on - strand, within PfGW456L13_2665 at 2964.834 kb on + strand, within PfGW456L13_2665 at 2964.919 kb on - strand, within PfGW456L13_2665 at 2964.919 kb on - strand, within PfGW456L13_2665 at 2964.937 kb on - strand at 2964.949 kb on + strand at 2964.949 kb on + strand at 2964.949 kb on + strand at 2964.949 kb on + strand at 2964.949 kb on + strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2964.950 kb on - strand at 2965.033 kb on + strand at 2965.033 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.035 kb on + strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.036 kb on - strand at 2965.044 kb on + strand at 2965.044 kb on + strand at 2965.044 kb on + strand at 2965.045 kb on - strand at 2965.075 kb on + strand at 2965.075 kb on + strand at 2965.195 kb on - strand, within PfGW456L13_2666 at 2965.293 kb on + strand, within PfGW456L13_2666 at 2965.293 kb on + strand, within PfGW456L13_2666 at 2965.293 kb on + strand, within PfGW456L13_2666 at 2965.329 kb on + strand, within PfGW456L13_2666 at 2965.330 kb on - strand, within PfGW456L13_2666 at 2965.330 kb on - strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.392 kb on + strand, within PfGW456L13_2666 at 2965.393 kb on - strand, within PfGW456L13_2666 at 2965.393 kb on - strand, within PfGW456L13_2666 at 2965.395 kb on + strand, within PfGW456L13_2666 at 2965.395 kb on + strand, within PfGW456L13_2666 at 2965.395 kb on + strand, within PfGW456L13_2666 at 2965.396 kb on - strand, within PfGW456L13_2666 at 2965.396 kb on - strand, within PfGW456L13_2666 at 2965.396 kb on - strand, within PfGW456L13_2666 at 2965.453 kb on + strand at 2965.572 kb on - strand at 2965.593 kb on + strand at 2965.594 kb on - strand at 2965.639 kb on - strand, within PfGW456L13_2667 at 2965.666 kb on - strand, within PfGW456L13_2667 at 2965.666 kb on - strand, within PfGW456L13_2667 at 2965.678 kb on + strand, within PfGW456L13_2667 at 2965.680 kb on + strand, within PfGW456L13_2667 at 2965.681 kb on - strand, within PfGW456L13_2667 at 2965.681 kb on - strand, within PfGW456L13_2667 at 2965.681 kb on - strand, within PfGW456L13_2667
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,963,463 + -0.5 2,963,463 + +1.0 2,963,642 + -0.5 2,963,642 + +1.0 2,963,642 + -0.0 2,963,642 + -1.3 2,963,643 - +0.2 2,963,643 - +1.1 2,963,643 - -0.7 2,963,644 + -0.4 2,963,645 - +1.3 2,963,645 - +0.2 2,963,878 + PfGW456L13_2663 0.35 +0.4 2,963,878 + PfGW456L13_2663 0.35 -1.5 2,963,878 + PfGW456L13_2663 0.35 -0.4 2,963,878 + PfGW456L13_2663 0.35 +0.6 2,963,878 + PfGW456L13_2663 0.35 +0.7 2,963,879 - PfGW456L13_2663 0.35 -0.0 2,963,879 - PfGW456L13_2663 0.35 +0.3 2,963,879 - PfGW456L13_2663 0.35 -3.4 2,963,879 - PfGW456L13_2663 0.35 +0.8 2,963,879 - PfGW456L13_2663 0.35 -0.3 2,963,879 - PfGW456L13_2663 0.35 +1.0 2,963,879 - PfGW456L13_2663 0.35 +0.4 2,963,904 + PfGW456L13_2663 0.39 -0.2 2,963,904 + PfGW456L13_2663 0.39 -1.0 2,963,905 - PfGW456L13_2663 0.39 -0.3 2,963,977 + PfGW456L13_2663 0.49 +0.9 2,964,021 + PfGW456L13_2663 0.55 +0.4 2,964,021 + PfGW456L13_2663 0.55 -0.6 2,964,022 - PfGW456L13_2663 0.55 -0.3 2,964,022 - PfGW456L13_2663 0.55 -0.5 2,964,138 - PfGW456L13_2663 0.72 -0.0 2,964,164 + PfGW456L13_2663 0.76 -0.6 2,964,164 + PfGW456L13_2663 0.76 -0.1 2,964,164 + PfGW456L13_2663 0.76 -0.3 2,964,165 - PfGW456L13_2663 0.76 -2.0 2,964,264 + PfGW456L13_2663 0.90 -0.3 2,964,265 - +0.7 2,964,265 - +1.2 2,964,265 - -0.4 2,964,371 - +1.0 2,964,390 - -1.0 2,964,455 + PfGW456L13_2664 0.28 -1.5 2,964,456 - PfGW456L13_2664 0.29 -0.4 2,964,478 + PfGW456L13_2664 0.35 -1.4 2,964,479 - PfGW456L13_2664 0.36 -0.4 2,964,479 - PfGW456L13_2664 0.36 -2.0 2,964,479 - PfGW456L13_2664 0.36 -0.5 2,964,711 + +0.2 2,964,777 + PfGW456L13_2665 0.27 +1.0 2,964,778 - PfGW456L13_2665 0.27 -1.2 2,964,778 - PfGW456L13_2665 0.27 +0.1 2,964,778 - PfGW456L13_2665 0.27 -2.5 2,964,778 - PfGW456L13_2665 0.27 +0.7 2,964,778 - PfGW456L13_2665 0.27 +0.5 2,964,778 - PfGW456L13_2665 0.27 -0.8 2,964,778 - PfGW456L13_2665 0.27 -1.8 2,964,834 + PfGW456L13_2665 0.50 -0.2 2,964,919 - PfGW456L13_2665 0.84 -0.4 2,964,919 - PfGW456L13_2665 0.84 -0.5 2,964,937 - +0.4 2,964,949 + +0.3 2,964,949 + -0.3 2,964,949 + -1.6 2,964,949 + -0.9 2,964,949 + -0.5 2,964,950 - +1.3 2,964,950 - -2.0 2,964,950 - +0.5 2,964,950 - -0.8 2,964,950 - -1.5 2,964,950 - -0.8 2,964,950 - +0.8 2,965,033 + -0.1 2,965,033 + -0.8 2,965,035 + +0.0 2,965,035 + -0.1 2,965,035 + +0.3 2,965,035 + -1.9 2,965,035 + -2.0 2,965,035 + -0.2 2,965,035 + -1.3 2,965,035 + +0.5 2,965,035 + -0.5 2,965,036 - -0.0 2,965,036 - +0.5 2,965,036 - -0.0 2,965,036 - -0.0 2,965,036 - -0.6 2,965,036 - -1.0 2,965,036 - +0.1 2,965,036 - -0.4 2,965,036 - +0.1 2,965,036 - -0.0 2,965,036 - +0.2 2,965,036 - +1.4 2,965,036 - +0.2 2,965,036 - -0.4 2,965,044 + -0.0 2,965,044 + -0.7 2,965,044 + +2.0 2,965,045 - +0.1 2,965,075 + +2.8 2,965,075 + -1.5 2,965,195 - PfGW456L13_2666 0.25 -1.0 2,965,293 + PfGW456L13_2666 0.53 -1.5 2,965,293 + PfGW456L13_2666 0.53 -0.3 2,965,293 + PfGW456L13_2666 0.53 -0.0 2,965,329 + PfGW456L13_2666 0.63 +1.8 2,965,330 - PfGW456L13_2666 0.64 +1.4 2,965,330 - PfGW456L13_2666 0.64 -0.9 2,965,392 + PfGW456L13_2666 0.81 +2.5 2,965,392 + PfGW456L13_2666 0.81 +0.4 2,965,392 + PfGW456L13_2666 0.81 +2.2 2,965,392 + PfGW456L13_2666 0.81 +0.5 2,965,392 + PfGW456L13_2666 0.81 +1.6 2,965,392 + PfGW456L13_2666 0.81 -0.5 2,965,392 + PfGW456L13_2666 0.81 -1.3 2,965,392 + PfGW456L13_2666 0.81 +0.6 2,965,392 + PfGW456L13_2666 0.81 -0.7 2,965,393 - PfGW456L13_2666 0.81 -2.1 2,965,393 - PfGW456L13_2666 0.81 -0.0 2,965,395 + PfGW456L13_2666 0.82 -1.0 2,965,395 + PfGW456L13_2666 0.82 -0.4 2,965,395 + PfGW456L13_2666 0.82 -0.8 2,965,396 - PfGW456L13_2666 0.82 +0.4 2,965,396 - PfGW456L13_2666 0.82 +1.0 2,965,396 - PfGW456L13_2666 0.82 +0.8 2,965,453 + -0.0 2,965,572 - +0.4 2,965,593 + -1.5 2,965,594 - +0.4 2,965,639 - PfGW456L13_2667 0.12 +0.5 2,965,666 - PfGW456L13_2667 0.18 +1.0 2,965,666 - PfGW456L13_2667 0.18 +1.5 2,965,678 + PfGW456L13_2667 0.21 +0.4 2,965,680 + PfGW456L13_2667 0.21 -1.8 2,965,681 - PfGW456L13_2667 0.22 -1.0 2,965,681 - PfGW456L13_2667 0.22 +0.5 2,965,681 - PfGW456L13_2667 0.22 -1.8
Or see this region's nucleotide sequence