Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2337 and PfGW456L13_2338 are separated by 3 nucleotides PfGW456L13_2338 and PfGW456L13_2339 overlap by 4 nucleotides
PfGW456L13_2337 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 2,568,424 to 2,569,866
_2337
PfGW456L13_2338 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 2,569,870 to 2,570,841
_2338
PfGW456L13_2339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,570,838 to 2,571,773
_2339
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2569.091 kb on + strand, within PfGW456L13_2337 at 2569.301 kb on + strand, within PfGW456L13_2337 at 2569.363 kb on - strand, within PfGW456L13_2337 at 2569.391 kb on + strand, within PfGW456L13_2337 at 2569.679 kb on + strand, within PfGW456L13_2337 at 2569.679 kb on + strand, within PfGW456L13_2337 at 2569.679 kb on + strand, within PfGW456L13_2337 at 2569.679 kb on + strand, within PfGW456L13_2337 at 2569.679 kb on + strand, within PfGW456L13_2337 at 2569.680 kb on - strand, within PfGW456L13_2337 at 2569.680 kb on - strand, within PfGW456L13_2337 at 2569.680 kb on - strand, within PfGW456L13_2337 at 2569.680 kb on - strand, within PfGW456L13_2337 at 2569.680 kb on - strand, within PfGW456L13_2337 at 2569.682 kb on + strand, within PfGW456L13_2337 at 2569.682 kb on + strand, within PfGW456L13_2337 at 2569.683 kb on - strand, within PfGW456L13_2337 at 2569.683 kb on - strand, within PfGW456L13_2337 at 2569.871 kb on + strand at 2569.871 kb on + strand at 2569.924 kb on - strand at 2570.043 kb on - strand, within PfGW456L13_2338 at 2570.165 kb on + strand, within PfGW456L13_2338 at 2570.165 kb on + strand, within PfGW456L13_2338 at 2570.166 kb on - strand, within PfGW456L13_2338 at 2570.166 kb on - strand, within PfGW456L13_2338 at 2570.166 kb on - strand, within PfGW456L13_2338 at 2570.351 kb on + strand, within PfGW456L13_2338 at 2570.351 kb on + strand, within PfGW456L13_2338 at 2570.408 kb on + strand, within PfGW456L13_2338 at 2570.477 kb on + strand, within PfGW456L13_2338 at 2570.606 kb on + strand, within PfGW456L13_2338 at 2570.606 kb on + strand, within PfGW456L13_2338 at 2570.606 kb on + strand, within PfGW456L13_2338 at 2570.606 kb on + strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.607 kb on - strand, within PfGW456L13_2338 at 2570.679 kb on - strand, within PfGW456L13_2338 at 2570.762 kb on + strand at 2570.793 kb on - strand at 2570.793 kb on - strand at 2570.814 kb on + strand at 2570.814 kb on + strand at 2570.815 kb on - strand at 2570.815 kb on - strand at 2570.835 kb on + strand at 2570.836 kb on - strand at 2571.079 kb on + strand, within PfGW456L13_2339 at 2571.079 kb on + strand, within PfGW456L13_2339 at 2571.080 kb on - strand, within PfGW456L13_2339 at 2571.080 kb on - strand, within PfGW456L13_2339 at 2571.095 kb on + strand, within PfGW456L13_2339 at 2571.096 kb on - strand, within PfGW456L13_2339 at 2571.362 kb on - strand, within PfGW456L13_2339 at 2571.362 kb on - strand, within PfGW456L13_2339 at 2571.427 kb on + strand, within PfGW456L13_2339 at 2571.428 kb on - strand, within PfGW456L13_2339 at 2571.449 kb on - strand, within PfGW456L13_2339 at 2571.519 kb on - strand, within PfGW456L13_2339 at 2571.711 kb on + strand at 2571.769 kb on + strand at 2571.769 kb on + strand at 2571.769 kb on + strand at 2571.769 kb on + strand at 2571.769 kb on + strand at 2571.769 kb on + strand at 2571.770 kb on - strand at 2571.788 kb on - strand at 2571.796 kb on - strand at 2571.831 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,569,091 + PfGW456L13_2337 0.46 +0.3 2,569,301 + PfGW456L13_2337 0.61 +1.0 2,569,363 - PfGW456L13_2337 0.65 +0.8 2,569,391 + PfGW456L13_2337 0.67 -2.0 2,569,679 + PfGW456L13_2337 0.87 -0.4 2,569,679 + PfGW456L13_2337 0.87 +0.1 2,569,679 + PfGW456L13_2337 0.87 +0.9 2,569,679 + PfGW456L13_2337 0.87 +0.2 2,569,679 + PfGW456L13_2337 0.87 -0.1 2,569,680 - PfGW456L13_2337 0.87 +0.2 2,569,680 - PfGW456L13_2337 0.87 +0.9 2,569,680 - PfGW456L13_2337 0.87 +0.2 2,569,680 - PfGW456L13_2337 0.87 +0.7 2,569,680 - PfGW456L13_2337 0.87 +0.6 2,569,682 + PfGW456L13_2337 0.87 +0.4 2,569,682 + PfGW456L13_2337 0.87 -0.3 2,569,683 - PfGW456L13_2337 0.87 +0.2 2,569,683 - PfGW456L13_2337 0.87 +1.0 2,569,871 + -0.2 2,569,871 + +1.0 2,569,924 - +1.7 2,570,043 - PfGW456L13_2338 0.18 +1.9 2,570,165 + PfGW456L13_2338 0.30 +0.3 2,570,165 + PfGW456L13_2338 0.30 -0.3 2,570,166 - PfGW456L13_2338 0.30 +1.6 2,570,166 - PfGW456L13_2338 0.30 -0.0 2,570,166 - PfGW456L13_2338 0.30 +1.4 2,570,351 + PfGW456L13_2338 0.49 +1.1 2,570,351 + PfGW456L13_2338 0.49 -0.7 2,570,408 + PfGW456L13_2338 0.55 +1.1 2,570,477 + PfGW456L13_2338 0.62 -0.0 2,570,606 + PfGW456L13_2338 0.76 +0.9 2,570,606 + PfGW456L13_2338 0.76 -0.3 2,570,606 + PfGW456L13_2338 0.76 +0.2 2,570,606 + PfGW456L13_2338 0.76 -0.6 2,570,607 - PfGW456L13_2338 0.76 +0.6 2,570,607 - PfGW456L13_2338 0.76 -1.0 2,570,607 - PfGW456L13_2338 0.76 +0.5 2,570,607 - PfGW456L13_2338 0.76 +0.2 2,570,607 - PfGW456L13_2338 0.76 -2.8 2,570,607 - PfGW456L13_2338 0.76 -0.7 2,570,679 - PfGW456L13_2338 0.83 +0.7 2,570,762 + +1.4 2,570,793 - -0.1 2,570,793 - +0.3 2,570,814 + -0.6 2,570,814 + -2.8 2,570,815 - +1.2 2,570,815 - -0.5 2,570,835 + -0.7 2,570,836 - +0.2 2,571,079 + PfGW456L13_2339 0.26 -2.3 2,571,079 + PfGW456L13_2339 0.26 +0.3 2,571,080 - PfGW456L13_2339 0.26 -0.1 2,571,080 - PfGW456L13_2339 0.26 -1.4 2,571,095 + PfGW456L13_2339 0.27 +0.3 2,571,096 - PfGW456L13_2339 0.28 -0.0 2,571,362 - PfGW456L13_2339 0.56 -0.3 2,571,362 - PfGW456L13_2339 0.56 -1.0 2,571,427 + PfGW456L13_2339 0.63 +1.6 2,571,428 - PfGW456L13_2339 0.63 -0.1 2,571,449 - PfGW456L13_2339 0.65 -0.1 2,571,519 - PfGW456L13_2339 0.73 +0.6 2,571,711 + -1.0 2,571,769 + -0.7 2,571,769 + -0.8 2,571,769 + -1.0 2,571,769 + +0.8 2,571,769 + +1.6 2,571,769 + -2.7 2,571,770 - -4.2 2,571,788 - -2.0 2,571,796 - -1.6 2,571,831 + -2.2
Or see this region's nucleotide sequence