Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_2338

Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_2337 and PfGW456L13_2338 are separated by 3 nucleotidesPfGW456L13_2338 and PfGW456L13_2339 overlap by 4 nucleotides PfGW456L13_2337 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 2,568,424 to 2,569,866 _2337 PfGW456L13_2338 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 2,569,870 to 2,570,841 _2338 PfGW456L13_2339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,570,838 to 2,571,773 _2339 Position (kb) 2569 2570 2571Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2569.091 kb on + strand, within PfGW456L13_2337at 2569.301 kb on + strand, within PfGW456L13_2337at 2569.363 kb on - strand, within PfGW456L13_2337at 2569.391 kb on + strand, within PfGW456L13_2337at 2569.679 kb on + strand, within PfGW456L13_2337at 2569.679 kb on + strand, within PfGW456L13_2337at 2569.679 kb on + strand, within PfGW456L13_2337at 2569.679 kb on + strand, within PfGW456L13_2337at 2569.679 kb on + strand, within PfGW456L13_2337at 2569.680 kb on - strand, within PfGW456L13_2337at 2569.680 kb on - strand, within PfGW456L13_2337at 2569.680 kb on - strand, within PfGW456L13_2337at 2569.680 kb on - strand, within PfGW456L13_2337at 2569.680 kb on - strand, within PfGW456L13_2337at 2569.682 kb on + strand, within PfGW456L13_2337at 2569.682 kb on + strand, within PfGW456L13_2337at 2569.683 kb on - strand, within PfGW456L13_2337at 2569.683 kb on - strand, within PfGW456L13_2337at 2569.871 kb on + strandat 2569.871 kb on + strandat 2569.924 kb on - strandat 2570.043 kb on - strand, within PfGW456L13_2338at 2570.165 kb on + strand, within PfGW456L13_2338at 2570.165 kb on + strand, within PfGW456L13_2338at 2570.166 kb on - strand, within PfGW456L13_2338at 2570.166 kb on - strand, within PfGW456L13_2338at 2570.166 kb on - strand, within PfGW456L13_2338at 2570.351 kb on + strand, within PfGW456L13_2338at 2570.351 kb on + strand, within PfGW456L13_2338at 2570.408 kb on + strand, within PfGW456L13_2338at 2570.477 kb on + strand, within PfGW456L13_2338at 2570.606 kb on + strand, within PfGW456L13_2338at 2570.606 kb on + strand, within PfGW456L13_2338at 2570.606 kb on + strand, within PfGW456L13_2338at 2570.606 kb on + strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.607 kb on - strand, within PfGW456L13_2338at 2570.679 kb on - strand, within PfGW456L13_2338at 2570.762 kb on + strandat 2570.793 kb on - strandat 2570.793 kb on - strandat 2570.814 kb on + strandat 2570.814 kb on + strandat 2570.815 kb on - strandat 2570.815 kb on - strandat 2570.835 kb on + strandat 2570.836 kb on - strandat 2571.079 kb on + strand, within PfGW456L13_2339at 2571.079 kb on + strand, within PfGW456L13_2339at 2571.080 kb on - strand, within PfGW456L13_2339at 2571.080 kb on - strand, within PfGW456L13_2339at 2571.095 kb on + strand, within PfGW456L13_2339at 2571.096 kb on - strand, within PfGW456L13_2339at 2571.362 kb on - strand, within PfGW456L13_2339at 2571.362 kb on - strand, within PfGW456L13_2339at 2571.427 kb on + strand, within PfGW456L13_2339at 2571.428 kb on - strand, within PfGW456L13_2339at 2571.449 kb on - strand, within PfGW456L13_2339at 2571.519 kb on - strand, within PfGW456L13_2339at 2571.711 kb on + strandat 2571.769 kb on + strandat 2571.769 kb on + strandat 2571.769 kb on + strandat 2571.769 kb on + strandat 2571.769 kb on + strandat 2571.769 kb on + strandat 2571.770 kb on - strandat 2571.788 kb on - strandat 2571.796 kb on - strandat 2571.831 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
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2,569,091 + PfGW456L13_2337 0.46 +0.3
2,569,301 + PfGW456L13_2337 0.61 +1.0
2,569,363 - PfGW456L13_2337 0.65 +0.8
2,569,391 + PfGW456L13_2337 0.67 -2.0
2,569,679 + PfGW456L13_2337 0.87 -0.4
2,569,679 + PfGW456L13_2337 0.87 +0.1
2,569,679 + PfGW456L13_2337 0.87 +0.9
2,569,679 + PfGW456L13_2337 0.87 +0.2
2,569,679 + PfGW456L13_2337 0.87 -0.1
2,569,680 - PfGW456L13_2337 0.87 +0.2
2,569,680 - PfGW456L13_2337 0.87 +0.9
2,569,680 - PfGW456L13_2337 0.87 +0.2
2,569,680 - PfGW456L13_2337 0.87 +0.7
2,569,680 - PfGW456L13_2337 0.87 +0.6
2,569,682 + PfGW456L13_2337 0.87 +0.4
2,569,682 + PfGW456L13_2337 0.87 -0.3
2,569,683 - PfGW456L13_2337 0.87 +0.2
2,569,683 - PfGW456L13_2337 0.87 +1.0
2,569,871 + -0.2
2,569,871 + +1.0
2,569,924 - +1.7
2,570,043 - PfGW456L13_2338 0.18 +1.9
2,570,165 + PfGW456L13_2338 0.30 +0.3
2,570,165 + PfGW456L13_2338 0.30 -0.3
2,570,166 - PfGW456L13_2338 0.30 +1.6
2,570,166 - PfGW456L13_2338 0.30 -0.0
2,570,166 - PfGW456L13_2338 0.30 +1.4
2,570,351 + PfGW456L13_2338 0.49 +1.1
2,570,351 + PfGW456L13_2338 0.49 -0.7
2,570,408 + PfGW456L13_2338 0.55 +1.1
2,570,477 + PfGW456L13_2338 0.62 -0.0
2,570,606 + PfGW456L13_2338 0.76 +0.9
2,570,606 + PfGW456L13_2338 0.76 -0.3
2,570,606 + PfGW456L13_2338 0.76 +0.2
2,570,606 + PfGW456L13_2338 0.76 -0.6
2,570,607 - PfGW456L13_2338 0.76 +0.6
2,570,607 - PfGW456L13_2338 0.76 -1.0
2,570,607 - PfGW456L13_2338 0.76 +0.5
2,570,607 - PfGW456L13_2338 0.76 +0.2
2,570,607 - PfGW456L13_2338 0.76 -2.8
2,570,607 - PfGW456L13_2338 0.76 -0.7
2,570,679 - PfGW456L13_2338 0.83 +0.7
2,570,762 + +1.4
2,570,793 - -0.1
2,570,793 - +0.3
2,570,814 + -0.6
2,570,814 + -2.8
2,570,815 - +1.2
2,570,815 - -0.5
2,570,835 + -0.7
2,570,836 - +0.2
2,571,079 + PfGW456L13_2339 0.26 -2.3
2,571,079 + PfGW456L13_2339 0.26 +0.3
2,571,080 - PfGW456L13_2339 0.26 -0.1
2,571,080 - PfGW456L13_2339 0.26 -1.4
2,571,095 + PfGW456L13_2339 0.27 +0.3
2,571,096 - PfGW456L13_2339 0.28 -0.0
2,571,362 - PfGW456L13_2339 0.56 -0.3
2,571,362 - PfGW456L13_2339 0.56 -1.0
2,571,427 + PfGW456L13_2339 0.63 +1.6
2,571,428 - PfGW456L13_2339 0.63 -0.1
2,571,449 - PfGW456L13_2339 0.65 -0.1
2,571,519 - PfGW456L13_2339 0.73 +0.6
2,571,711 + -1.0
2,571,769 + -0.7
2,571,769 + -0.8
2,571,769 + -1.0
2,571,769 + +0.8
2,571,769 + +1.6
2,571,769 + -2.7
2,571,770 - -4.2
2,571,788 - -2.0
2,571,796 - -1.6
2,571,831 + -2.2

Or see this region's nucleotide sequence