Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2307 and PfGW456L13_2308 are separated by 20 nucleotides PfGW456L13_2308 and PfGW456L13_2309 overlap by 4 nucleotides PfGW456L13_2309 and PfGW456L13_2310 overlap by 4 nucleotides PfGW456L13_2310 and PfGW456L13_2311 are separated by 289 nucleotides PfGW456L13_2311 and PfGW456L13_2312 are separated by 126 nucleotides
PfGW456L13_2307 - FIG049111: Hypothetical protein in pyoverdin gene cluster, at 2,534,711 to 2,535,262
_2307
PfGW456L13_2308 - FIG137877: Hypothetical protein in pyoverdin gene cluster, at 2,535,283 to 2,535,612
_2308
PfGW456L13_2309 - FIG139991: Putative thiamine pyrophosphate-requiring enzyme, at 2,535,609 to 2,536,151
_2309
PfGW456L13_2310 - FIG137594: Putative iron-regulated membrane protein, at 2,536,148 to 2,537,350
_2310
PfGW456L13_2311 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 2,537,640 to 2,538,098
_2311
PfGW456L13_2312 - Redox-sensitive transcriptional activator SoxR, at 2,538,225 to 2,538,680
_2312
Position (kb)
2536
2537
2538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2535.228 kb on + strand at 2535.228 kb on + strand at 2535.228 kb on + strand at 2535.228 kb on + strand at 2535.228 kb on + strand at 2535.228 kb on + strand at 2535.229 kb on - strand at 2535.229 kb on - strand at 2535.229 kb on - strand at 2535.350 kb on + strand, within PfGW456L13_2308 at 2535.350 kb on + strand, within PfGW456L13_2308 at 2535.350 kb on + strand, within PfGW456L13_2308 at 2535.350 kb on + strand, within PfGW456L13_2308 at 2535.350 kb on + strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.351 kb on - strand at 2535.351 kb on - strand, within PfGW456L13_2308 at 2535.838 kb on + strand, within PfGW456L13_2309 at 2535.838 kb on + strand, within PfGW456L13_2309 at 2535.838 kb on + strand, within PfGW456L13_2309 at 2535.838 kb on + strand, within PfGW456L13_2309 at 2535.839 kb on - strand, within PfGW456L13_2309 at 2535.839 kb on - strand, within PfGW456L13_2309 at 2535.839 kb on - strand, within PfGW456L13_2309 at 2535.861 kb on - strand, within PfGW456L13_2309 at 2536.036 kb on + strand, within PfGW456L13_2309 at 2536.036 kb on + strand, within PfGW456L13_2309 at 2536.037 kb on - strand, within PfGW456L13_2309 at 2536.037 kb on - strand, within PfGW456L13_2309 at 2536.438 kb on - strand, within PfGW456L13_2310 at 2536.438 kb on - strand, within PfGW456L13_2310 at 2536.506 kb on + strand, within PfGW456L13_2310 at 2536.506 kb on + strand, within PfGW456L13_2310 at 2536.507 kb on - strand, within PfGW456L13_2310 at 2536.507 kb on - strand, within PfGW456L13_2310 at 2536.808 kb on - strand, within PfGW456L13_2310 at 2536.808 kb on - strand, within PfGW456L13_2310 at 2536.848 kb on + strand, within PfGW456L13_2310 at 2536.849 kb on - strand, within PfGW456L13_2310 at 2536.849 kb on - strand, within PfGW456L13_2310 at 2536.908 kb on + strand, within PfGW456L13_2310 at 2536.909 kb on - strand, within PfGW456L13_2310 at 2536.909 kb on - strand, within PfGW456L13_2310 at 2537.031 kb on + strand, within PfGW456L13_2310 at 2537.031 kb on + strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.032 kb on - strand, within PfGW456L13_2310 at 2537.059 kb on - strand, within PfGW456L13_2310 at 2537.163 kb on + strand, within PfGW456L13_2310 at 2537.163 kb on + strand, within PfGW456L13_2310 at 2537.171 kb on + strand, within PfGW456L13_2310 at 2537.172 kb on - strand, within PfGW456L13_2310 at 2537.308 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.456 kb on + strand at 2537.457 kb on - strand at 2537.457 kb on - strand at 2537.457 kb on - strand at 2537.457 kb on - strand at 2537.460 kb on + strand at 2537.461 kb on - strand at 2537.691 kb on - strand, within PfGW456L13_2311 at 2537.765 kb on - strand, within PfGW456L13_2311 at 2537.778 kb on - strand, within PfGW456L13_2311 at 2537.788 kb on - strand, within PfGW456L13_2311 at 2537.788 kb on - strand, within PfGW456L13_2311 at 2537.821 kb on + strand, within PfGW456L13_2311 at 2537.845 kb on + strand, within PfGW456L13_2311 at 2537.845 kb on + strand, within PfGW456L13_2311 at 2537.846 kb on - strand, within PfGW456L13_2311 at 2537.846 kb on - strand, within PfGW456L13_2311 at 2538.071 kb on - strand at 2538.135 kb on + strand at 2538.135 kb on + strand at 2538.150 kb on - strand at 2538.150 kb on - strand at 2538.150 kb on - strand at 2538.150 kb on - strand at 2538.163 kb on + strand at 2538.163 kb on + strand at 2538.163 kb on + strand at 2538.163 kb on + strand at 2538.163 kb on + strand at 2538.164 kb on - strand at 2538.164 kb on - strand at 2538.164 kb on - strand at 2538.164 kb on - strand at 2538.164 kb on - strand at 2538.164 kb on - strand at 2538.165 kb on + strand at 2538.165 kb on + strand at 2538.165 kb on + strand at 2538.165 kb on + strand at 2538.165 kb on + strand at 2538.165 kb on + strand at 2538.166 kb on - strand at 2538.166 kb on - strand at 2538.166 kb on - strand at 2538.224 kb on - strand at 2538.224 kb on - strand at 2538.224 kb on - strand at 2538.327 kb on - strand, within PfGW456L13_2312
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,535,228 + +0.6 2,535,228 + -0.5 2,535,228 + +0.2 2,535,228 + -1.8 2,535,228 + -0.8 2,535,228 + +0.4 2,535,229 - +1.0 2,535,229 - -0.0 2,535,229 - -0.7 2,535,350 + PfGW456L13_2308 0.20 -0.4 2,535,350 + PfGW456L13_2308 0.20 -0.3 2,535,350 + PfGW456L13_2308 0.20 -0.6 2,535,350 + PfGW456L13_2308 0.20 -0.0 2,535,350 + PfGW456L13_2308 0.20 +0.6 2,535,351 - PfGW456L13_2308 0.21 -0.6 2,535,351 - PfGW456L13_2308 0.21 -0.7 2,535,351 - PfGW456L13_2308 0.21 -0.4 2,535,351 - PfGW456L13_2308 0.21 -2.0 2,535,351 - PfGW456L13_2308 0.21 +0.6 2,535,351 - PfGW456L13_2308 0.21 -1.4 2,535,351 - PfGW456L13_2308 0.21 +0.7 2,535,351 - PfGW456L13_2308 0.21 -0.7 2,535,351 - PfGW456L13_2308 0.21 -0.0 2,535,351 - PfGW456L13_2308 0.21 -0.9 2,535,351 - PfGW456L13_2308 0.21 +1.0 2,535,351 - +2.0 2,535,351 - PfGW456L13_2308 0.21 -2.6 2,535,838 + PfGW456L13_2309 0.42 +0.3 2,535,838 + PfGW456L13_2309 0.42 +0.1 2,535,838 + PfGW456L13_2309 0.42 -0.7 2,535,838 + PfGW456L13_2309 0.42 -0.5 2,535,839 - PfGW456L13_2309 0.42 -0.0 2,535,839 - PfGW456L13_2309 0.42 -0.8 2,535,839 - PfGW456L13_2309 0.42 +0.4 2,535,861 - PfGW456L13_2309 0.46 +0.1 2,536,036 + PfGW456L13_2309 0.79 -0.5 2,536,036 + PfGW456L13_2309 0.79 -0.7 2,536,037 - PfGW456L13_2309 0.79 -1.5 2,536,037 - PfGW456L13_2309 0.79 +0.4 2,536,438 - PfGW456L13_2310 0.24 +0.5 2,536,438 - PfGW456L13_2310 0.24 +0.1 2,536,506 + PfGW456L13_2310 0.30 -0.3 2,536,506 + PfGW456L13_2310 0.30 +0.5 2,536,507 - PfGW456L13_2310 0.30 +1.3 2,536,507 - PfGW456L13_2310 0.30 +0.4 2,536,808 - PfGW456L13_2310 0.55 +0.8 2,536,808 - PfGW456L13_2310 0.55 +1.1 2,536,848 + PfGW456L13_2310 0.58 -1.4 2,536,849 - PfGW456L13_2310 0.58 -0.5 2,536,849 - PfGW456L13_2310 0.58 +1.7 2,536,908 + PfGW456L13_2310 0.63 +0.1 2,536,909 - PfGW456L13_2310 0.63 -0.3 2,536,909 - PfGW456L13_2310 0.63 +0.4 2,537,031 + PfGW456L13_2310 0.73 +0.2 2,537,031 + PfGW456L13_2310 0.73 -2.5 2,537,032 - PfGW456L13_2310 0.73 +0.7 2,537,032 - PfGW456L13_2310 0.73 -0.3 2,537,032 - PfGW456L13_2310 0.73 -0.0 2,537,032 - PfGW456L13_2310 0.73 +0.3 2,537,032 - PfGW456L13_2310 0.73 -1.3 2,537,032 - PfGW456L13_2310 0.73 +0.3 2,537,059 - PfGW456L13_2310 0.76 +1.2 2,537,163 + PfGW456L13_2310 0.84 +0.3 2,537,163 + PfGW456L13_2310 0.84 -1.4 2,537,171 + PfGW456L13_2310 0.85 -0.2 2,537,172 - PfGW456L13_2310 0.85 -2.3 2,537,308 + -0.5 2,537,456 + -0.0 2,537,456 + -0.7 2,537,456 + +0.1 2,537,456 + -0.0 2,537,456 + +0.5 2,537,456 + +1.3 2,537,456 + +0.2 2,537,456 + -1.5 2,537,456 + -0.8 2,537,457 - -1.2 2,537,457 - -0.0 2,537,457 - -3.0 2,537,457 - -0.3 2,537,460 + +0.5 2,537,461 - -3.5 2,537,691 - PfGW456L13_2311 0.11 +0.8 2,537,765 - PfGW456L13_2311 0.27 +0.6 2,537,778 - PfGW456L13_2311 0.30 -0.5 2,537,788 - PfGW456L13_2311 0.32 +0.2 2,537,788 - PfGW456L13_2311 0.32 -0.0 2,537,821 + PfGW456L13_2311 0.39 +0.5 2,537,845 + PfGW456L13_2311 0.45 +0.1 2,537,845 + PfGW456L13_2311 0.45 +0.4 2,537,846 - PfGW456L13_2311 0.45 -0.0 2,537,846 - PfGW456L13_2311 0.45 -0.7 2,538,071 - +0.6 2,538,135 + -0.5 2,538,135 + -1.0 2,538,150 - -1.3 2,538,150 - -2.3 2,538,150 - -0.0 2,538,150 - +0.7 2,538,163 + -1.0 2,538,163 + +1.0 2,538,163 + +0.1 2,538,163 + -0.2 2,538,163 + -1.0 2,538,164 - -0.2 2,538,164 - +0.9 2,538,164 - +0.0 2,538,164 - -0.8 2,538,164 - -0.4 2,538,164 - +0.0 2,538,165 + -0.7 2,538,165 + -0.6 2,538,165 + -0.7 2,538,165 + -1.0 2,538,165 + +0.5 2,538,165 + +0.8 2,538,166 - +0.7 2,538,166 - +0.0 2,538,166 - -1.5 2,538,224 - +0.6 2,538,224 - -1.3 2,538,224 - -0.3 2,538,327 - PfGW456L13_2312 0.22 -0.2
Or see this region's nucleotide sequence