Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2181 and PfGW456L13_2182 are separated by 360 nucleotides PfGW456L13_2182 and PfGW456L13_2183 are separated by 426 nucleotides
PfGW456L13_2181 - Integration host factor beta subunit, at 2,391,409 to 2,391,705
_2181
PfGW456L13_2182 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,392,066 to 2,393,136
_2182
PfGW456L13_2183 - WzxE protein, at 2,393,563 to 2,394,822
_2183
Position (kb)
2392
2393
2394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2391.111 kb on - strand at 2391.238 kb on + strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.239 kb on - strand at 2391.318 kb on + strand at 2391.318 kb on + strand at 2391.319 kb on - strand at 2391.319 kb on - strand at 2391.325 kb on + strand at 2391.572 kb on - strand, within PfGW456L13_2181 at 2391.572 kb on - strand, within PfGW456L13_2181 at 2391.763 kb on + strand at 2391.764 kb on - strand at 2391.764 kb on - strand at 2391.766 kb on + strand at 2391.766 kb on + strand at 2391.767 kb on - strand at 2391.842 kb on + strand at 2391.861 kb on + strand at 2391.862 kb on - strand at 2391.862 kb on - strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.863 kb on + strand at 2391.864 kb on - strand at 2391.864 kb on - strand at 2392.042 kb on + strand at 2392.050 kb on - strand at 2392.106 kb on + strand at 2392.106 kb on + strand at 2392.194 kb on - strand, within PfGW456L13_2182 at 2392.219 kb on + strand, within PfGW456L13_2182 at 2392.219 kb on + strand, within PfGW456L13_2182 at 2392.272 kb on + strand, within PfGW456L13_2182 at 2392.335 kb on - strand, within PfGW456L13_2182 at 2392.467 kb on + strand, within PfGW456L13_2182 at 2392.467 kb on + strand, within PfGW456L13_2182 at 2392.468 kb on - strand, within PfGW456L13_2182 at 2392.468 kb on - strand, within PfGW456L13_2182 at 2392.469 kb on + strand, within PfGW456L13_2182 at 2392.469 kb on + strand, within PfGW456L13_2182 at 2392.470 kb on - strand, within PfGW456L13_2182 at 2392.470 kb on - strand, within PfGW456L13_2182 at 2392.558 kb on + strand, within PfGW456L13_2182 at 2392.558 kb on + strand, within PfGW456L13_2182 at 2392.559 kb on - strand, within PfGW456L13_2182 at 2392.559 kb on - strand, within PfGW456L13_2182 at 2392.707 kb on - strand, within PfGW456L13_2182 at 2392.707 kb on - strand, within PfGW456L13_2182 at 2392.728 kb on + strand, within PfGW456L13_2182 at 2392.790 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.813 kb on + strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.814 kb on - strand, within PfGW456L13_2182 at 2392.815 kb on + strand, within PfGW456L13_2182 at 2392.964 kb on - strand, within PfGW456L13_2182 at 2393.083 kb on + strand at 2393.130 kb on + strand at 2393.131 kb on - strand at 2393.244 kb on + strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.245 kb on - strand at 2393.259 kb on - strand at 2393.334 kb on + strand at 2393.334 kb on + strand at 2393.344 kb on + strand at 2393.359 kb on + strand at 2393.360 kb on - strand at 2393.363 kb on + strand at 2393.363 kb on + strand at 2393.375 kb on + strand at 2393.376 kb on - strand at 2393.376 kb on - strand at 2393.380 kb on - strand at 2393.380 kb on - strand at 2393.410 kb on + strand at 2393.417 kb on + strand at 2393.421 kb on - strand at 2393.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,391,111 - -0.2 2,391,238 + -3.0 2,391,239 - +2.6 2,391,239 - +2.0 2,391,239 - +0.4 2,391,239 - -0.6 2,391,318 + +0.0 2,391,318 + -1.0 2,391,319 - +0.9 2,391,319 - +1.6 2,391,325 + -3.5 2,391,572 - PfGW456L13_2181 0.55 -1.0 2,391,572 - PfGW456L13_2181 0.55 +0.7 2,391,763 + +0.0 2,391,764 - -2.8 2,391,764 - -1.0 2,391,766 + -3.2 2,391,766 + -2.6 2,391,767 - +0.0 2,391,842 + -3.8 2,391,861 + -4.6 2,391,862 - -2.0 2,391,862 - +0.3 2,391,863 + -2.3 2,391,863 + -3.2 2,391,863 + -1.6 2,391,863 + -1.6 2,391,863 + -3.0 2,391,864 - -0.8 2,391,864 - +0.0 2,392,042 + -2.7 2,392,050 - -2.0 2,392,106 + +0.8 2,392,106 + +0.7 2,392,194 - PfGW456L13_2182 0.12 -0.7 2,392,219 + PfGW456L13_2182 0.14 +1.7 2,392,219 + PfGW456L13_2182 0.14 +2.5 2,392,272 + PfGW456L13_2182 0.19 +2.6 2,392,335 - PfGW456L13_2182 0.25 -3.3 2,392,467 + PfGW456L13_2182 0.37 +1.5 2,392,467 + PfGW456L13_2182 0.37 +1.0 2,392,468 - PfGW456L13_2182 0.38 +1.2 2,392,468 - PfGW456L13_2182 0.38 -1.0 2,392,469 + PfGW456L13_2182 0.38 +0.5 2,392,469 + PfGW456L13_2182 0.38 +1.9 2,392,470 - PfGW456L13_2182 0.38 -1.6 2,392,470 - PfGW456L13_2182 0.38 -0.2 2,392,558 + PfGW456L13_2182 0.46 +1.1 2,392,558 + PfGW456L13_2182 0.46 +1.7 2,392,559 - PfGW456L13_2182 0.46 -0.2 2,392,559 - PfGW456L13_2182 0.46 -2.3 2,392,707 - PfGW456L13_2182 0.60 -2.0 2,392,707 - PfGW456L13_2182 0.60 +0.0 2,392,728 + PfGW456L13_2182 0.62 +3.5 2,392,790 + PfGW456L13_2182 0.68 +1.9 2,392,813 + PfGW456L13_2182 0.70 +1.0 2,392,813 + PfGW456L13_2182 0.70 +2.5 2,392,813 + PfGW456L13_2182 0.70 +0.7 2,392,813 + PfGW456L13_2182 0.70 +2.3 2,392,814 - PfGW456L13_2182 0.70 +0.0 2,392,814 - PfGW456L13_2182 0.70 +0.0 2,392,814 - PfGW456L13_2182 0.70 -0.8 2,392,815 + PfGW456L13_2182 0.70 +1.7 2,392,964 - PfGW456L13_2182 0.84 +0.0 2,393,083 + +2.9 2,393,130 + +0.3 2,393,131 - -1.4 2,393,244 + +0.6 2,393,245 - +0.6 2,393,245 - +0.0 2,393,245 - -1.0 2,393,245 - -1.0 2,393,259 - -1.6 2,393,334 + +2.0 2,393,334 + +1.8 2,393,344 + +0.0 2,393,359 + -1.6 2,393,360 - -2.4 2,393,363 + -0.6 2,393,363 + -1.0 2,393,375 + +0.3 2,393,376 - -1.0 2,393,376 - -0.2 2,393,380 - +1.0 2,393,380 - -2.1 2,393,410 + +0.3 2,393,417 + +1.7 2,393,421 - -3.0 2,393,471 - -1.6
Or see this region's nucleotide sequence