Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_2161 and PfGW456L13_2162 are separated by 12 nucleotides PfGW456L13_2162 and PfGW456L13_2163 are separated by 102 nucleotides PfGW456L13_2163 and PfGW456L13_2164 are separated by 3 nucleotides PfGW456L13_2164 and PfGW456L13_2165 overlap by 8 nucleotides
PfGW456L13_2161 - FIG00954136: hypothetical protein, at 2,365,351 to 2,365,875
_2161
PfGW456L13_2162 - Cell division protein BolA, at 2,365,888 to 2,366,187
_2162
PfGW456L13_2163 - Rhodanese domain protein UPF0176, at 2,366,290 to 2,367,231
_2163
PfGW456L13_2164 - Thioredoxin, at 2,367,235 to 2,367,870
_2164
PfGW456L13_2165 - Lipid A export ATP-binding/permease protein MsbA, at 2,367,863 to 2,369,680
_2165
Position (kb)
2366
2367
2368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2365.323 kb on + strand at 2365.323 kb on + strand at 2365.324 kb on - strand at 2365.324 kb on - strand at 2365.324 kb on - strand at 2365.324 kb on - strand at 2365.336 kb on - strand at 2365.432 kb on - strand, within PfGW456L13_2161 at 2365.492 kb on + strand, within PfGW456L13_2161 at 2365.492 kb on + strand, within PfGW456L13_2161 at 2365.492 kb on + strand, within PfGW456L13_2161 at 2365.492 kb on + strand, within PfGW456L13_2161 at 2365.492 kb on + strand, within PfGW456L13_2161 at 2365.493 kb on - strand, within PfGW456L13_2161 at 2365.493 kb on - strand, within PfGW456L13_2161 at 2365.493 kb on - strand, within PfGW456L13_2161 at 2365.493 kb on - strand, within PfGW456L13_2161 at 2365.493 kb on - strand, within PfGW456L13_2161 at 2365.502 kb on + strand, within PfGW456L13_2161 at 2365.502 kb on + strand, within PfGW456L13_2161 at 2365.502 kb on + strand, within PfGW456L13_2161 at 2365.502 kb on + strand, within PfGW456L13_2161 at 2365.503 kb on - strand, within PfGW456L13_2161 at 2365.503 kb on - strand, within PfGW456L13_2161 at 2365.574 kb on - strand, within PfGW456L13_2161 at 2365.574 kb on - strand, within PfGW456L13_2161 at 2365.648 kb on + strand, within PfGW456L13_2161 at 2365.648 kb on + strand, within PfGW456L13_2161 at 2365.648 kb on + strand, within PfGW456L13_2161 at 2365.648 kb on + strand, within PfGW456L13_2161 at 2365.649 kb on - strand, within PfGW456L13_2161 at 2365.649 kb on - strand, within PfGW456L13_2161 at 2365.649 kb on - strand, within PfGW456L13_2161 at 2365.696 kb on + strand, within PfGW456L13_2161 at 2365.696 kb on + strand, within PfGW456L13_2161 at 2365.696 kb on + strand, within PfGW456L13_2161 at 2365.697 kb on - strand, within PfGW456L13_2161 at 2365.873 kb on + strand at 2365.887 kb on + strand at 2365.887 kb on + strand at 2365.887 kb on + strand at 2365.887 kb on + strand at 2366.059 kb on + strand, within PfGW456L13_2162 at 2366.059 kb on + strand, within PfGW456L13_2162 at 2366.060 kb on - strand, within PfGW456L13_2162 at 2366.060 kb on - strand, within PfGW456L13_2162 at 2366.109 kb on + strand, within PfGW456L13_2162 at 2366.109 kb on + strand, within PfGW456L13_2162 at 2366.110 kb on - strand, within PfGW456L13_2162 at 2366.110 kb on - strand, within PfGW456L13_2162 at 2366.110 kb on - strand, within PfGW456L13_2162 at 2366.110 kb on - strand, within PfGW456L13_2162 at 2366.113 kb on + strand, within PfGW456L13_2162 at 2366.113 kb on + strand, within PfGW456L13_2162 at 2366.113 kb on + strand, within PfGW456L13_2162 at 2366.113 kb on + strand, within PfGW456L13_2162 at 2366.113 kb on + strand, within PfGW456L13_2162 at 2366.114 kb on - strand, within PfGW456L13_2162 at 2366.114 kb on - strand, within PfGW456L13_2162 at 2366.114 kb on - strand, within PfGW456L13_2162 at 2366.114 kb on - strand, within PfGW456L13_2162 at 2366.203 kb on + strand at 2366.204 kb on - strand at 2366.285 kb on + strand at 2366.285 kb on + strand at 2366.285 kb on + strand at 2366.286 kb on - strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.320 kb on + strand at 2366.321 kb on - strand at 2366.321 kb on - strand at 2366.321 kb on - strand at 2366.321 kb on - strand at 2366.321 kb on - strand at 2366.322 kb on + strand at 2366.322 kb on + strand at 2366.323 kb on - strand at 2366.323 kb on - strand at 2366.323 kb on - strand at 2366.323 kb on - strand at 2366.344 kb on + strand at 2366.344 kb on + strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.345 kb on - strand at 2366.525 kb on - strand, within PfGW456L13_2163 at 2366.546 kb on - strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.626 kb on + strand, within PfGW456L13_2163 at 2366.627 kb on - strand, within PfGW456L13_2163 at 2366.627 kb on - strand, within PfGW456L13_2163 at 2366.627 kb on - strand, within PfGW456L13_2163 at 2366.698 kb on + strand, within PfGW456L13_2163 at 2366.698 kb on + strand, within PfGW456L13_2163 at 2366.699 kb on - strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.857 kb on + strand, within PfGW456L13_2163 at 2366.858 kb on - strand, within PfGW456L13_2163 at 2366.858 kb on - strand, within PfGW456L13_2163 at 2366.858 kb on - strand, within PfGW456L13_2163 at 2366.915 kb on - strand, within PfGW456L13_2163 at 2367.014 kb on - strand, within PfGW456L13_2163 at 2367.073 kb on + strand, within PfGW456L13_2163 at 2367.073 kb on + strand, within PfGW456L13_2163 at 2367.073 kb on + strand, within PfGW456L13_2163 at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.253 kb on + strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.254 kb on - strand at 2367.409 kb on + strand, within PfGW456L13_2164 at 2367.409 kb on + strand at 2367.589 kb on + strand, within PfGW456L13_2164 at 2367.590 kb on + strand, within PfGW456L13_2164 at 2367.590 kb on - strand, within PfGW456L13_2164 at 2367.590 kb on - strand, within PfGW456L13_2164 at 2367.775 kb on - strand, within PfGW456L13_2164 at 2367.979 kb on - strand at 2368.121 kb on + strand, within PfGW456L13_2165 at 2368.121 kb on + strand, within PfGW456L13_2165 at 2368.226 kb on + strand, within PfGW456L13_2165
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,365,323 + -0.8 2,365,323 + +0.0 2,365,324 - -1.0 2,365,324 - -1.1 2,365,324 - -0.5 2,365,324 - -0.7 2,365,336 - -1.1 2,365,432 - PfGW456L13_2161 0.15 -0.2 2,365,492 + PfGW456L13_2161 0.27 +1.6 2,365,492 + PfGW456L13_2161 0.27 -0.6 2,365,492 + PfGW456L13_2161 0.27 +0.0 2,365,492 + PfGW456L13_2161 0.27 +0.0 2,365,492 + PfGW456L13_2161 0.27 +0.4 2,365,493 - PfGW456L13_2161 0.27 +0.3 2,365,493 - PfGW456L13_2161 0.27 -3.0 2,365,493 - PfGW456L13_2161 0.27 -0.6 2,365,493 - PfGW456L13_2161 0.27 -2.2 2,365,493 - PfGW456L13_2161 0.27 -1.6 2,365,502 + PfGW456L13_2161 0.29 +0.3 2,365,502 + PfGW456L13_2161 0.29 -0.3 2,365,502 + PfGW456L13_2161 0.29 +0.0 2,365,502 + PfGW456L13_2161 0.29 -0.1 2,365,503 - PfGW456L13_2161 0.29 -2.1 2,365,503 - PfGW456L13_2161 0.29 -0.8 2,365,574 - PfGW456L13_2161 0.42 -0.4 2,365,574 - PfGW456L13_2161 0.42 -1.7 2,365,648 + PfGW456L13_2161 0.57 -0.3 2,365,648 + PfGW456L13_2161 0.57 +0.0 2,365,648 + PfGW456L13_2161 0.57 +0.1 2,365,648 + PfGW456L13_2161 0.57 +0.3 2,365,649 - PfGW456L13_2161 0.57 -1.2 2,365,649 - PfGW456L13_2161 0.57 -0.3 2,365,649 - PfGW456L13_2161 0.57 +0.7 2,365,696 + PfGW456L13_2161 0.66 +0.6 2,365,696 + PfGW456L13_2161 0.66 -2.4 2,365,696 + PfGW456L13_2161 0.66 -0.3 2,365,697 - PfGW456L13_2161 0.66 -2.0 2,365,873 + +0.3 2,365,887 + +0.6 2,365,887 + -0.3 2,365,887 + +0.3 2,365,887 + -0.3 2,366,059 + PfGW456L13_2162 0.57 -1.2 2,366,059 + PfGW456L13_2162 0.57 -1.3 2,366,060 - PfGW456L13_2162 0.57 -2.1 2,366,060 - PfGW456L13_2162 0.57 +0.9 2,366,109 + PfGW456L13_2162 0.74 +0.5 2,366,109 + PfGW456L13_2162 0.74 -1.0 2,366,110 - PfGW456L13_2162 0.74 +0.9 2,366,110 - PfGW456L13_2162 0.74 +1.2 2,366,110 - PfGW456L13_2162 0.74 -0.8 2,366,110 - PfGW456L13_2162 0.74 -0.5 2,366,113 + PfGW456L13_2162 0.75 -0.3 2,366,113 + PfGW456L13_2162 0.75 -1.0 2,366,113 + PfGW456L13_2162 0.75 +0.2 2,366,113 + PfGW456L13_2162 0.75 +0.2 2,366,113 + PfGW456L13_2162 0.75 +0.0 2,366,114 - PfGW456L13_2162 0.75 -0.8 2,366,114 - PfGW456L13_2162 0.75 -0.2 2,366,114 - PfGW456L13_2162 0.75 -0.5 2,366,114 - PfGW456L13_2162 0.75 +1.1 2,366,203 + +0.7 2,366,204 - +0.6 2,366,285 + +1.2 2,366,285 + -0.4 2,366,285 + -0.3 2,366,286 - -0.6 2,366,320 + -2.2 2,366,320 + -0.6 2,366,320 + -1.3 2,366,320 + -2.0 2,366,320 + -1.8 2,366,320 + -0.5 2,366,320 + +0.6 2,366,321 - +0.0 2,366,321 - -0.7 2,366,321 - +0.7 2,366,321 - +0.0 2,366,321 - -2.1 2,366,322 + -0.6 2,366,322 + -1.0 2,366,323 - -0.2 2,366,323 - -1.6 2,366,323 - -1.6 2,366,323 - +0.0 2,366,344 + +0.0 2,366,344 + +0.3 2,366,345 - -0.8 2,366,345 - -0.7 2,366,345 - +1.5 2,366,345 - -0.4 2,366,345 - -2.3 2,366,345 - -3.0 2,366,345 - -1.8 2,366,525 - PfGW456L13_2163 0.25 -1.2 2,366,546 - PfGW456L13_2163 0.27 -2.3 2,366,626 + PfGW456L13_2163 0.36 +0.6 2,366,626 + PfGW456L13_2163 0.36 -1.0 2,366,626 + PfGW456L13_2163 0.36 -2.6 2,366,626 + PfGW456L13_2163 0.36 +1.0 2,366,626 + PfGW456L13_2163 0.36 -1.6 2,366,626 + PfGW456L13_2163 0.36 +0.4 2,366,627 - PfGW456L13_2163 0.36 -1.0 2,366,627 - PfGW456L13_2163 0.36 +0.0 2,366,627 - PfGW456L13_2163 0.36 +0.0 2,366,698 + PfGW456L13_2163 0.43 +0.0 2,366,698 + PfGW456L13_2163 0.43 -1.0 2,366,699 - PfGW456L13_2163 0.43 +0.4 2,366,857 + PfGW456L13_2163 0.60 -0.6 2,366,857 + PfGW456L13_2163 0.60 -0.4 2,366,857 + PfGW456L13_2163 0.60 +0.3 2,366,857 + PfGW456L13_2163 0.60 -1.6 2,366,857 + PfGW456L13_2163 0.60 +1.0 2,366,857 + PfGW456L13_2163 0.60 +1.0 2,366,858 - PfGW456L13_2163 0.60 +0.3 2,366,858 - PfGW456L13_2163 0.60 -2.3 2,366,858 - PfGW456L13_2163 0.60 -1.4 2,366,915 - PfGW456L13_2163 0.66 -2.8 2,367,014 - PfGW456L13_2163 0.77 +0.0 2,367,073 + PfGW456L13_2163 0.83 -0.4 2,367,073 + PfGW456L13_2163 0.83 -1.0 2,367,073 + PfGW456L13_2163 0.83 +0.0 2,367,253 + +0.5 2,367,253 + +1.6 2,367,253 + +0.8 2,367,253 + +0.3 2,367,253 + -1.4 2,367,253 + +0.8 2,367,253 + +0.5 2,367,253 + -0.7 2,367,253 + -0.8 2,367,253 + -0.1 2,367,253 + -0.4 2,367,253 + -0.4 2,367,254 - -0.3 2,367,254 - -0.6 2,367,254 - -1.3 2,367,254 - -0.4 2,367,254 - -0.3 2,367,254 - -1.9 2,367,254 - -0.1 2,367,254 - -0.2 2,367,409 + PfGW456L13_2164 0.27 -2.3 2,367,409 + +0.0 2,367,589 + PfGW456L13_2164 0.56 -1.8 2,367,590 + PfGW456L13_2164 0.56 -1.0 2,367,590 - PfGW456L13_2164 0.56 +0.6 2,367,590 - PfGW456L13_2164 0.56 +0.0 2,367,775 - PfGW456L13_2164 0.85 +1.8 2,367,979 - +0.2 2,368,121 + PfGW456L13_2165 0.14 +1.0 2,368,121 + PfGW456L13_2165 0.14 +0.1 2,368,226 + PfGW456L13_2165 0.20 -0.3
Or see this region's nucleotide sequence