Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1988 and PfGW456L13_1989 are separated by 95 nucleotides PfGW456L13_1989 and PfGW456L13_1990 are separated by 561 nucleotides PfGW456L13_1990 and PfGW456L13_1991 overlap by 15 nucleotides PfGW456L13_1991 and PfGW456L13_1992 overlap by 4 nucleotides PfGW456L13_1992 and PfGW456L13_1993 are separated by 99 nucleotides
PfGW456L13_1988 - Putative two-component system response regulator, at 2,192,565 to 2,193,179
_1988
PfGW456L13_1989 - hypothetical protein, at 2,193,275 to 2,193,463
_1989
PfGW456L13_1990 - hypothetical protein, at 2,194,025 to 2,194,168
_1990
PfGW456L13_1991 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 2,194,154 to 2,195,758
_1991
PfGW456L13_1992 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,195,755 to 2,196,447
_1992
PfGW456L13_1993 - glutamine synthetase family protein, at 2,196,547 to 2,197,893
_1993
Position (kb)
2194
2195
2196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2193.222 kb on + strand at 2193.223 kb on - strand at 2193.483 kb on - strand at 2193.514 kb on + strand at 2193.514 kb on + strand at 2193.515 kb on - strand at 2193.515 kb on - strand at 2193.520 kb on + strand at 2193.520 kb on + strand at 2193.520 kb on + strand at 2193.520 kb on + strand at 2193.520 kb on + strand at 2193.521 kb on - strand at 2193.521 kb on - strand at 2193.521 kb on - strand at 2193.524 kb on + strand at 2193.524 kb on + strand at 2193.524 kb on + strand at 2193.524 kb on + strand at 2193.524 kb on + strand at 2193.536 kb on - strand at 2193.536 kb on - strand at 2193.576 kb on + strand at 2193.577 kb on - strand at 2193.577 kb on - strand at 2193.577 kb on - strand at 2193.577 kb on - strand at 2193.624 kb on - strand at 2193.656 kb on - strand at 2193.656 kb on - strand at 2193.671 kb on + strand at 2193.672 kb on - strand at 2193.672 kb on - strand at 2193.691 kb on - strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.693 kb on + strand at 2193.694 kb on - strand at 2193.694 kb on - strand at 2193.694 kb on - strand at 2193.771 kb on + strand at 2193.781 kb on - strand at 2193.850 kb on - strand at 2193.850 kb on - strand at 2193.850 kb on - strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.866 kb on + strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.867 kb on - strand at 2193.910 kb on + strand at 2193.910 kb on + strand at 2193.911 kb on - strand at 2193.911 kb on - strand at 2193.916 kb on + strand at 2193.917 kb on - strand at 2193.917 kb on - strand at 2193.917 kb on - strand at 2193.969 kb on + strand at 2194.026 kb on + strand at 2194.027 kb on - strand at 2194.049 kb on - strand at 2194.109 kb on + strand, within PfGW456L13_1990 at 2194.110 kb on - strand, within PfGW456L13_1990 at 2194.110 kb on - strand, within PfGW456L13_1990 at 2194.133 kb on + strand, within PfGW456L13_1990 at 2194.133 kb on + strand, within PfGW456L13_1990 at 2194.134 kb on - strand, within PfGW456L13_1990 at 2194.163 kb on + strand at 2194.164 kb on - strand at 2194.164 kb on - strand at 2194.164 kb on - strand at 2194.307 kb on + strand at 2194.307 kb on + strand at 2194.307 kb on + strand at 2194.308 kb on - strand at 2194.308 kb on - strand at 2194.308 kb on - strand at 2194.411 kb on - strand, within PfGW456L13_1991 at 2194.482 kb on - strand, within PfGW456L13_1991 at 2194.633 kb on + strand, within PfGW456L13_1991 at 2194.633 kb on + strand, within PfGW456L13_1991 at 2194.634 kb on - strand, within PfGW456L13_1991 at 2194.715 kb on + strand, within PfGW456L13_1991 at 2194.716 kb on - strand, within PfGW456L13_1991 at 2194.716 kb on - strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.810 kb on + strand, within PfGW456L13_1991 at 2194.811 kb on - strand, within PfGW456L13_1991 at 2194.811 kb on - strand, within PfGW456L13_1991 at 2194.811 kb on - strand, within PfGW456L13_1991 at 2194.811 kb on - strand, within PfGW456L13_1991 at 2194.814 kb on + strand, within PfGW456L13_1991 at 2194.814 kb on + strand, within PfGW456L13_1991 at 2194.814 kb on + strand, within PfGW456L13_1991 at 2194.815 kb on - strand, within PfGW456L13_1991 at 2194.856 kb on + strand, within PfGW456L13_1991 at 2194.857 kb on - strand, within PfGW456L13_1991 at 2194.875 kb on + strand, within PfGW456L13_1991 at 2194.875 kb on + strand, within PfGW456L13_1991 at 2194.875 kb on + strand, within PfGW456L13_1991 at 2194.875 kb on + strand, within PfGW456L13_1991 at 2194.875 kb on + strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2194.876 kb on - strand, within PfGW456L13_1991 at 2195.031 kb on + strand, within PfGW456L13_1991 at 2195.032 kb on - strand, within PfGW456L13_1991 at 2195.240 kb on - strand, within PfGW456L13_1991 at 2195.517 kb on - strand, within PfGW456L13_1991 at 2195.517 kb on - strand, within PfGW456L13_1991 at 2195.517 kb on - strand, within PfGW456L13_1991 at 2195.551 kb on - strand, within PfGW456L13_1991 at 2195.590 kb on + strand, within PfGW456L13_1991 at 2195.814 kb on + strand at 2195.814 kb on + strand at 2195.814 kb on + strand at 2195.815 kb on - strand at 2195.815 kb on - strand at 2195.962 kb on + strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2195.963 kb on - strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.082 kb on + strand, within PfGW456L13_1992 at 2196.083 kb on - strand, within PfGW456L13_1992 at 2196.083 kb on - strand, within PfGW456L13_1992 at 2196.083 kb on - strand, within PfGW456L13_1992 at 2196.083 kb on - strand, within PfGW456L13_1992 at 2196.083 kb on - strand, within PfGW456L13_1992 at 2196.286 kb on + strand, within PfGW456L13_1992 at 2196.287 kb on - strand, within PfGW456L13_1992 at 2196.334 kb on + strand, within PfGW456L13_1992 at 2196.334 kb on + strand, within PfGW456L13_1992 at 2196.335 kb on - strand, within PfGW456L13_1992 at 2196.335 kb on - strand, within PfGW456L13_1992 at 2196.436 kb on + strand at 2196.437 kb on - strand at 2196.437 kb on - strand at 2196.497 kb on + strand at 2196.497 kb on + strand at 2196.497 kb on + strand at 2196.498 kb on - strand at 2196.531 kb on + strand at 2196.531 kb on + strand at 2196.532 kb on - strand at 2196.532 kb on - strand at 2196.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 2,193,222 + -0.7 2,193,223 - -0.3 2,193,483 - -0.4 2,193,514 + +0.1 2,193,514 + +1.4 2,193,515 - -0.3 2,193,515 - +0.8 2,193,520 + -0.4 2,193,520 + -0.5 2,193,520 + -0.0 2,193,520 + -1.1 2,193,520 + +0.3 2,193,521 - -0.1 2,193,521 - +0.7 2,193,521 - +0.7 2,193,524 + -2.7 2,193,524 + +0.8 2,193,524 + +0.5 2,193,524 + +0.8 2,193,524 + -0.2 2,193,536 - -1.4 2,193,536 - +0.7 2,193,576 + -0.9 2,193,577 - -0.2 2,193,577 - -0.5 2,193,577 - +2.3 2,193,577 - +0.2 2,193,624 - +0.1 2,193,656 - +1.0 2,193,656 - -0.8 2,193,671 + -0.4 2,193,672 - -0.0 2,193,672 - +0.3 2,193,691 - +0.3 2,193,693 + -0.2 2,193,693 + -2.0 2,193,693 + -0.6 2,193,693 + -0.9 2,193,693 + -0.4 2,193,693 + -0.1 2,193,693 + -0.2 2,193,693 + +0.8 2,193,693 + -0.7 2,193,693 + +1.4 2,193,694 - +0.3 2,193,694 - -1.9 2,193,694 - +2.2 2,193,771 + +1.3 2,193,781 - +0.8 2,193,850 - -1.0 2,193,850 - -0.3 2,193,850 - +0.5 2,193,866 + +1.6 2,193,866 + -0.8 2,193,866 + -0.1 2,193,866 + -0.4 2,193,866 + +0.2 2,193,866 + -1.2 2,193,866 + -0.3 2,193,867 - +0.3 2,193,867 - -0.0 2,193,867 - -2.8 2,193,867 - -1.9 2,193,867 - -1.7 2,193,867 - -2.2 2,193,867 - -0.6 2,193,867 - -0.0 2,193,910 + +1.0 2,193,910 + -0.0 2,193,911 - +0.5 2,193,911 - -0.8 2,193,916 + -0.8 2,193,917 - -0.0 2,193,917 - -2.4 2,193,917 - -0.0 2,193,969 + +2.8 2,194,026 + -0.1 2,194,027 - +0.1 2,194,049 - -0.0 2,194,109 + PfGW456L13_1990 0.58 -0.3 2,194,110 - PfGW456L13_1990 0.59 +1.2 2,194,110 - PfGW456L13_1990 0.59 -0.4 2,194,133 + PfGW456L13_1990 0.75 -1.3 2,194,133 + PfGW456L13_1990 0.75 -0.7 2,194,134 - PfGW456L13_1990 0.76 +0.3 2,194,163 + +2.0 2,194,164 - -0.8 2,194,164 - +0.2 2,194,164 - -1.0 2,194,307 + +0.3 2,194,307 + -1.3 2,194,307 + +0.7 2,194,308 - -0.9 2,194,308 - +1.0 2,194,308 - -0.5 2,194,411 - PfGW456L13_1991 0.16 -0.1 2,194,482 - PfGW456L13_1991 0.20 -1.5 2,194,633 + PfGW456L13_1991 0.30 +0.7 2,194,633 + PfGW456L13_1991 0.30 +0.0 2,194,634 - PfGW456L13_1991 0.30 -0.1 2,194,715 + PfGW456L13_1991 0.35 +1.8 2,194,716 - PfGW456L13_1991 0.35 -2.0 2,194,716 - PfGW456L13_1991 0.35 -0.2 2,194,810 + PfGW456L13_1991 0.41 -0.0 2,194,810 + PfGW456L13_1991 0.41 -2.4 2,194,810 + PfGW456L13_1991 0.41 +1.2 2,194,810 + PfGW456L13_1991 0.41 -0.8 2,194,810 + PfGW456L13_1991 0.41 +0.5 2,194,810 + PfGW456L13_1991 0.41 +0.4 2,194,811 - PfGW456L13_1991 0.41 -0.4 2,194,811 - PfGW456L13_1991 0.41 +2.1 2,194,811 - PfGW456L13_1991 0.41 +0.5 2,194,811 - PfGW456L13_1991 0.41 +0.1 2,194,814 + PfGW456L13_1991 0.41 +0.3 2,194,814 + PfGW456L13_1991 0.41 -2.6 2,194,814 + PfGW456L13_1991 0.41 +1.1 2,194,815 - PfGW456L13_1991 0.41 -0.4 2,194,856 + PfGW456L13_1991 0.44 +0.6 2,194,857 - PfGW456L13_1991 0.44 +1.6 2,194,875 + PfGW456L13_1991 0.45 -1.4 2,194,875 + PfGW456L13_1991 0.45 -1.5 2,194,875 + PfGW456L13_1991 0.45 +1.0 2,194,875 + PfGW456L13_1991 0.45 +0.4 2,194,875 + PfGW456L13_1991 0.45 +1.1 2,194,876 - PfGW456L13_1991 0.45 +0.2 2,194,876 - PfGW456L13_1991 0.45 +0.3 2,194,876 - PfGW456L13_1991 0.45 -0.3 2,194,876 - PfGW456L13_1991 0.45 -0.1 2,194,876 - PfGW456L13_1991 0.45 -1.4 2,194,876 - PfGW456L13_1991 0.45 -0.3 2,194,876 - PfGW456L13_1991 0.45 +0.2 2,194,876 - PfGW456L13_1991 0.45 -1.6 2,194,876 - PfGW456L13_1991 0.45 +1.0 2,195,031 + PfGW456L13_1991 0.55 +0.0 2,195,032 - PfGW456L13_1991 0.55 +1.4 2,195,240 - PfGW456L13_1991 0.68 +0.0 2,195,517 - PfGW456L13_1991 0.85 -0.5 2,195,517 - PfGW456L13_1991 0.85 -0.6 2,195,517 - PfGW456L13_1991 0.85 -0.1 2,195,551 - PfGW456L13_1991 0.87 +0.8 2,195,590 + PfGW456L13_1991 0.89 +1.3 2,195,814 + +0.5 2,195,814 + -0.4 2,195,814 + -0.2 2,195,815 - +0.9 2,195,815 - +0.2 2,195,962 + PfGW456L13_1992 0.30 -0.2 2,195,963 - PfGW456L13_1992 0.30 -0.9 2,195,963 - PfGW456L13_1992 0.30 -0.0 2,195,963 - -1.6 2,195,963 - PfGW456L13_1992 0.30 -0.9 2,195,963 - PfGW456L13_1992 0.30 +1.2 2,195,963 - PfGW456L13_1992 0.30 +1.4 2,195,963 - PfGW456L13_1992 0.30 -0.4 2,195,963 - PfGW456L13_1992 0.30 -1.2 2,195,963 - PfGW456L13_1992 0.30 -0.1 2,196,082 + PfGW456L13_1992 0.47 -0.0 2,196,082 + PfGW456L13_1992 0.47 -1.5 2,196,082 + PfGW456L13_1992 0.47 +1.0 2,196,082 + PfGW456L13_1992 0.47 +1.6 2,196,082 + PfGW456L13_1992 0.47 +0.6 2,196,082 + PfGW456L13_1992 0.47 +1.2 2,196,082 + PfGW456L13_1992 0.47 -1.4 2,196,083 - PfGW456L13_1992 0.47 +0.9 2,196,083 - PfGW456L13_1992 0.47 -1.0 2,196,083 - PfGW456L13_1992 0.47 -0.1 2,196,083 - PfGW456L13_1992 0.47 +0.8 2,196,083 - PfGW456L13_1992 0.47 +1.0 2,196,286 + PfGW456L13_1992 0.77 +0.6 2,196,287 - PfGW456L13_1992 0.77 -1.0 2,196,334 + PfGW456L13_1992 0.84 +0.5 2,196,334 + PfGW456L13_1992 0.84 -0.5 2,196,335 - PfGW456L13_1992 0.84 -0.5 2,196,335 - PfGW456L13_1992 0.84 -1.8 2,196,436 + -2.4 2,196,437 - -0.0 2,196,437 - +1.8 2,196,497 + +0.0 2,196,497 + -0.9 2,196,497 + -0.1 2,196,498 - -0.6 2,196,531 + -0.5 2,196,531 + +0.1 2,196,532 - +1.6 2,196,532 - -0.5 2,196,632 + -0.0
Or see this region's nucleotide sequence