Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1768 and PfGW456L13_1769 are separated by 151 nucleotides PfGW456L13_1769 and PfGW456L13_1770 are separated by 4 nucleotides PfGW456L13_1770 and PfGW456L13_1771 are separated by 340 nucleotides
PfGW456L13_1768 - two-component system sensor protein, at 1,943,997 to 1,945,271
_1768
PfGW456L13_1769 - SAM-dependent methyltransferases, at 1,945,423 to 1,946,094
_1769
PfGW456L13_1770 - InaA protein, at 1,946,099 to 1,946,812
_1770
PfGW456L13_1771 - RND multidrug efflux transporter; Acriflavin resistance protein, at 1,947,153 to 1,950,179
_1771
Position (kb) 1946
1947 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1945.221 kb on - strand at 1945.277 kb on + strand at 1945.277 kb on + strand at 1945.278 kb on - strand at 1945.278 kb on - strand at 1945.278 kb on - strand at 1945.278 kb on - strand at 1945.278 kb on - strand at 1945.324 kb on + strand at 1945.325 kb on - strand at 1945.325 kb on - strand at 1945.347 kb on + strand at 1945.348 kb on - strand at 1945.380 kb on + strand at 1945.380 kb on + strand at 1945.381 kb on - strand at 1945.389 kb on + strand at 1945.389 kb on + strand at 1945.390 kb on - strand at 1945.394 kb on + strand at 1945.394 kb on + strand at 1945.395 kb on - strand at 1945.395 kb on - strand at 1945.395 kb on - strand at 1945.395 kb on - strand at 1945.463 kb on - strand at 1945.486 kb on + strand at 1945.751 kb on + strand, within PfGW456L13_1769 at 1945.751 kb on + strand, within PfGW456L13_1769 at 1945.751 kb on + strand, within PfGW456L13_1769 at 1945.843 kb on - strand, within PfGW456L13_1769 at 1945.960 kb on + strand, within PfGW456L13_1769 at 1945.989 kb on + strand, within PfGW456L13_1769 at 1946.056 kb on + strand at 1946.056 kb on + strand at 1946.056 kb on + strand at 1946.057 kb on - strand at 1946.057 kb on - strand at 1946.057 kb on - strand at 1946.057 kb on - strand at 1946.074 kb on + strand at 1946.074 kb on + strand at 1946.075 kb on - strand at 1946.075 kb on - strand at 1946.075 kb on - strand at 1946.075 kb on - strand at 1946.078 kb on + strand at 1946.078 kb on + strand at 1946.078 kb on + strand at 1946.078 kb on + strand at 1946.078 kb on + strand at 1946.079 kb on - strand at 1946.079 kb on - strand at 1946.079 kb on - strand at 1946.079 kb on - strand at 1946.079 kb on - strand at 1946.081 kb on + strand at 1946.082 kb on - strand at 1946.082 kb on - strand at 1946.092 kb on + strand at 1946.093 kb on - strand at 1946.093 kb on - strand at 1946.154 kb on - strand at 1946.181 kb on + strand, within PfGW456L13_1770 at 1946.181 kb on + strand, within PfGW456L13_1770 at 1946.181 kb on + strand, within PfGW456L13_1770 at 1946.182 kb on - strand, within PfGW456L13_1770 at 1946.255 kb on + strand, within PfGW456L13_1770 at 1946.255 kb on + strand, within PfGW456L13_1770 at 1946.279 kb on - strand, within PfGW456L13_1770 at 1946.287 kb on + strand, within PfGW456L13_1770 at 1946.287 kb on + strand, within PfGW456L13_1770 at 1946.288 kb on - strand, within PfGW456L13_1770 at 1946.511 kb on + strand, within PfGW456L13_1770 at 1946.512 kb on - strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.584 kb on + strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.585 kb on - strand, within PfGW456L13_1770 at 1946.588 kb on + strand, within PfGW456L13_1770 at 1946.589 kb on - strand, within PfGW456L13_1770 at 1946.589 kb on - strand, within PfGW456L13_1770 at 1946.601 kb on + strand, within PfGW456L13_1770 at 1946.601 kb on + strand, within PfGW456L13_1770 at 1946.602 kb on - strand, within PfGW456L13_1770 at 1946.602 kb on - strand, within PfGW456L13_1770 at 1946.602 kb on - strand, within PfGW456L13_1770 at 1946.768 kb on + strand at 1946.768 kb on + strand at 1946.775 kb on - strand at 1946.808 kb on + strand at 1946.809 kb on - strand at 1946.809 kb on - strand at 1946.849 kb on + strand at 1946.922 kb on - strand at 1947.091 kb on - strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.143 kb on + strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.144 kb on - strand at 1947.184 kb on + strand at 1947.185 kb on - strand at 1947.218 kb on + strand at 1947.219 kb on - strand at 1947.230 kb on + strand at 1947.231 kb on - strand at 1947.231 kb on - strand at 1947.231 kb on - strand at 1947.405 kb on + strand at 1947.405 kb on + strand at 1947.406 kb on - strand at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.484 kb on + strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.485 kb on - strand, within PfGW456L13_1771 at 1947.536 kb on + strand, within PfGW456L13_1771 at 1947.664 kb on + strand, within PfGW456L13_1771 at 1947.665 kb on - strand, within PfGW456L13_1771 at 1947.665 kb on - strand, within PfGW456L13_1771 at 1947.665 kb on - strand, within PfGW456L13_1771 at 1947.685 kb on + strand, within PfGW456L13_1771 at 1947.685 kb on + strand, within PfGW456L13_1771 at 1947.686 kb on - strand, within PfGW456L13_1771 at 1947.686 kb on - strand, within PfGW456L13_1771
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 1,945,221 - +0.5 1,945,277 + +1.4 1,945,277 + -2.3 1,945,278 - +1.1 1,945,278 - +0.6 1,945,278 - -0.3 1,945,278 - -0.8 1,945,278 - -0.6 1,945,324 + -0.0 1,945,325 - -1.2 1,945,325 - +0.7 1,945,347 + +1.0 1,945,348 - -0.8 1,945,380 + -0.6 1,945,380 + +1.0 1,945,381 - -1.4 1,945,389 + +0.4 1,945,389 + +0.4 1,945,390 - -1.8 1,945,394 + -0.6 1,945,394 + -0.6 1,945,395 - -0.0 1,945,395 - +2.0 1,945,395 - +0.4 1,945,395 - +0.3 1,945,463 - -0.8 1,945,486 + -0.4 1,945,751 + PfGW456L13_1769 0.49 -0.2 1,945,751 + PfGW456L13_1769 0.49 -1.1 1,945,751 + PfGW456L13_1769 0.49 +0.5 1,945,843 - PfGW456L13_1769 0.62 +0.1 1,945,960 + PfGW456L13_1769 0.80 -1.4 1,945,989 + PfGW456L13_1769 0.84 +0.8 1,946,056 + -0.0 1,946,056 + +0.2 1,946,056 + -0.0 1,946,057 - -0.5 1,946,057 - +0.1 1,946,057 - -0.0 1,946,057 - +1.7 1,946,074 + +0.3 1,946,074 + +0.5 1,946,075 - +1.6 1,946,075 - +1.8 1,946,075 - +1.4 1,946,075 - +1.9 1,946,078 + -0.6 1,946,078 + +1.0 1,946,078 + +1.0 1,946,078 + +0.2 1,946,078 + -0.8 1,946,079 - -0.2 1,946,079 - +0.8 1,946,079 - +0.7 1,946,079 - +0.7 1,946,079 - +1.6 1,946,081 + +0.8 1,946,082 - +0.8 1,946,082 - +3.1 1,946,092 + +1.0 1,946,093 - +0.8 1,946,093 - +1.6 1,946,154 - +0.2 1,946,181 + PfGW456L13_1770 0.11 +0.9 1,946,181 + PfGW456L13_1770 0.11 +0.1 1,946,181 + PfGW456L13_1770 0.11 +1.7 1,946,182 - PfGW456L13_1770 0.12 +0.6 1,946,255 + PfGW456L13_1770 0.22 +0.8 1,946,255 + PfGW456L13_1770 0.22 +0.8 1,946,279 - PfGW456L13_1770 0.25 +0.3 1,946,287 + PfGW456L13_1770 0.26 +0.6 1,946,287 + PfGW456L13_1770 0.26 +1.3 1,946,288 - PfGW456L13_1770 0.26 +1.0 1,946,511 + PfGW456L13_1770 0.58 +0.7 1,946,512 - PfGW456L13_1770 0.58 +0.5 1,946,584 + PfGW456L13_1770 0.68 +0.6 1,946,584 + PfGW456L13_1770 0.68 +0.6 1,946,584 + PfGW456L13_1770 0.68 +0.8 1,946,584 + PfGW456L13_1770 0.68 +1.7 1,946,584 + PfGW456L13_1770 0.68 +0.6 1,946,584 + PfGW456L13_1770 0.68 +1.2 1,946,584 + PfGW456L13_1770 0.68 +0.2 1,946,585 - PfGW456L13_1770 0.68 -1.2 1,946,585 - PfGW456L13_1770 0.68 +1.7 1,946,585 - PfGW456L13_1770 0.68 +0.9 1,946,585 - PfGW456L13_1770 0.68 +0.8 1,946,585 - PfGW456L13_1770 0.68 +1.1 1,946,585 - PfGW456L13_1770 0.68 +1.4 1,946,585 - PfGW456L13_1770 0.68 +0.3 1,946,588 + PfGW456L13_1770 0.68 +2.6 1,946,589 - PfGW456L13_1770 0.69 +1.2 1,946,589 - PfGW456L13_1770 0.69 +2.0 1,946,601 + PfGW456L13_1770 0.70 +0.5 1,946,601 + PfGW456L13_1770 0.70 +0.1 1,946,602 - PfGW456L13_1770 0.70 +1.0 1,946,602 - PfGW456L13_1770 0.70 +1.7 1,946,602 - PfGW456L13_1770 0.70 +2.3 1,946,768 + +0.3 1,946,768 + +0.7 1,946,775 - +1.1 1,946,808 + +0.8 1,946,809 - +1.1 1,946,809 - +1.0 1,946,849 + -0.3 1,946,922 - -0.2 1,947,091 - -0.0 1,947,143 + -0.7 1,947,143 + -0.0 1,947,143 + +1.3 1,947,143 + -1.2 1,947,143 + +0.6 1,947,143 + +0.7 1,947,143 + -0.0 1,947,143 + -0.2 1,947,143 + -0.7 1,947,143 + +0.7 1,947,143 + -0.4 1,947,143 + +0.9 1,947,143 + -0.9 1,947,143 + +1.8 1,947,143 + -0.8 1,947,144 - +0.7 1,947,144 - +1.3 1,947,144 - -1.0 1,947,144 - +1.3 1,947,144 - -0.4 1,947,144 - -0.3 1,947,144 - +0.7 1,947,144 - +0.3 1,947,184 + -0.6 1,947,185 - +1.0 1,947,218 + -0.6 1,947,219 - +0.5 1,947,230 + +1.0 1,947,231 - -0.6 1,947,231 - -0.8 1,947,231 - +0.1 1,947,405 + +0.5 1,947,405 + +1.0 1,947,406 - -1.2 1,947,484 + PfGW456L13_1771 0.11 -0.0 1,947,484 + PfGW456L13_1771 0.11 -1.6 1,947,484 + PfGW456L13_1771 0.11 +1.0 1,947,484 + PfGW456L13_1771 0.11 -1.0 1,947,484 + PfGW456L13_1771 0.11 +0.3 1,947,484 + PfGW456L13_1771 0.11 +1.3 1,947,484 + PfGW456L13_1771 0.11 -1.6 1,947,484 + PfGW456L13_1771 0.11 +1.0 1,947,484 + PfGW456L13_1771 0.11 +1.0 1,947,484 + PfGW456L13_1771 0.11 -0.8 1,947,484 + PfGW456L13_1771 0.11 +0.6 1,947,484 + PfGW456L13_1771 0.11 -0.0 1,947,484 + PfGW456L13_1771 0.11 -1.2 1,947,484 + PfGW456L13_1771 0.11 -0.0 1,947,485 - PfGW456L13_1771 0.11 +0.3 1,947,485 - PfGW456L13_1771 0.11 -1.3 1,947,485 - PfGW456L13_1771 0.11 -0.1 1,947,485 - PfGW456L13_1771 0.11 -1.7 1,947,485 - PfGW456L13_1771 0.11 -0.7 1,947,485 - PfGW456L13_1771 0.11 -0.0 1,947,485 - PfGW456L13_1771 0.11 +1.1 1,947,485 - PfGW456L13_1771 0.11 +0.4 1,947,485 - PfGW456L13_1771 0.11 -0.6 1,947,485 - PfGW456L13_1771 0.11 +0.2 1,947,485 - PfGW456L13_1771 0.11 -1.5 1,947,485 - PfGW456L13_1771 0.11 +0.2 1,947,485 - PfGW456L13_1771 0.11 +0.8 1,947,485 - PfGW456L13_1771 0.11 +0.6 1,947,485 - PfGW456L13_1771 0.11 -0.9 1,947,485 - PfGW456L13_1771 0.11 -0.1 1,947,536 + PfGW456L13_1771 0.13 +1.0 1,947,664 + PfGW456L13_1771 0.17 -1.6 1,947,665 - PfGW456L13_1771 0.17 -0.0 1,947,665 - PfGW456L13_1771 0.17 +0.6 1,947,665 - PfGW456L13_1771 0.17 -0.5 1,947,685 + PfGW456L13_1771 0.18 -0.4 1,947,685 + PfGW456L13_1771 0.18 +0.2 1,947,686 - PfGW456L13_1771 0.18 -0.0 1,947,686 - PfGW456L13_1771 0.18 +0.6
Or see this region's nucleotide sequence