Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_1284 and PfGW456L13_1285 are separated by 9 nucleotides PfGW456L13_1285 and PfGW456L13_1286 are separated by 162 nucleotides
PfGW456L13_1284 - Putative transport protein, at 1,423,838 to 1,425,235
_1284
PfGW456L13_1285 - Single-stranded DNA-binding protein, at 1,425,245 to 1,425,763
_1285
PfGW456L13_1286 - Transcriptional Regulator, AraC family, at 1,425,926 to 1,426,798
_1286
Position (kb)
1425
1426 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1425.007 kb on + strand, within PfGW456L13_1284 at 1425.026 kb on + strand, within PfGW456L13_1284 at 1425.026 kb on + strand, within PfGW456L13_1284 at 1425.228 kb on + strand at 1425.233 kb on + strand at 1425.780 kb on + strand at 1425.780 kb on + strand at 1425.781 kb on - strand at 1425.781 kb on - strand at 1425.806 kb on + strand at 1425.832 kb on + strand at 1425.888 kb on - strand at 1425.940 kb on + strand at 1425.940 kb on + strand at 1425.940 kb on + strand at 1425.940 kb on + strand at 1425.941 kb on - strand at 1425.941 kb on - strand at 1425.981 kb on - strand at 1425.981 kb on - strand at 1426.010 kb on + strand at 1426.010 kb on + strand at 1426.036 kb on + strand, within PfGW456L13_1286 at 1426.036 kb on + strand, within PfGW456L13_1286 at 1426.036 kb on + strand, within PfGW456L13_1286 at 1426.036 kb on + strand, within PfGW456L13_1286 at 1426.037 kb on - strand, within PfGW456L13_1286 at 1426.037 kb on - strand, within PfGW456L13_1286 at 1426.037 kb on - strand, within PfGW456L13_1286 at 1426.037 kb on - strand, within PfGW456L13_1286 at 1426.037 kb on - strand, within PfGW456L13_1286 at 1426.179 kb on + strand, within PfGW456L13_1286 at 1426.179 kb on + strand, within PfGW456L13_1286 at 1426.179 kb on + strand, within PfGW456L13_1286 at 1426.179 kb on + strand, within PfGW456L13_1286 at 1426.179 kb on + strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.180 kb on - strand, within PfGW456L13_1286 at 1426.213 kb on + strand, within PfGW456L13_1286 at 1426.221 kb on + strand, within PfGW456L13_1286 at 1426.221 kb on + strand, within PfGW456L13_1286 at 1426.458 kb on + strand, within PfGW456L13_1286 at 1426.458 kb on + strand, within PfGW456L13_1286 at 1426.459 kb on - strand, within PfGW456L13_1286 at 1426.536 kb on - strand, within PfGW456L13_1286 at 1426.536 kb on - strand, within PfGW456L13_1286 at 1426.536 kb on - strand, within PfGW456L13_1286 at 1426.580 kb on + strand, within PfGW456L13_1286 at 1426.581 kb on - strand, within PfGW456L13_1286 at 1426.581 kb on - strand, within PfGW456L13_1286 at 1426.581 kb on - strand, within PfGW456L13_1286 at 1426.665 kb on - strand, within PfGW456L13_1286
Per-strain Table
Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x remove 1,425,007 + PfGW456L13_1284 0.84 -1.0 1,425,026 + PfGW456L13_1284 0.85 -1.4 1,425,026 + PfGW456L13_1284 0.85 -1.0 1,425,228 + +1.2 1,425,233 + +0.3 1,425,780 + +0.7 1,425,780 + -0.6 1,425,781 - +1.8 1,425,781 - +0.5 1,425,806 + +0.6 1,425,832 + -1.0 1,425,888 - +0.8 1,425,940 + +0.9 1,425,940 + -0.1 1,425,940 + +0.8 1,425,940 + +1.1 1,425,941 - +0.4 1,425,941 - -0.8 1,425,981 - +0.4 1,425,981 - +0.9 1,426,010 + +0.6 1,426,010 + -1.2 1,426,036 + PfGW456L13_1286 0.13 -0.5 1,426,036 + PfGW456L13_1286 0.13 -0.9 1,426,036 + PfGW456L13_1286 0.13 -0.6 1,426,036 + PfGW456L13_1286 0.13 +0.5 1,426,037 - PfGW456L13_1286 0.13 +1.1 1,426,037 - PfGW456L13_1286 0.13 -0.6 1,426,037 - PfGW456L13_1286 0.13 -0.4 1,426,037 - PfGW456L13_1286 0.13 -3.5 1,426,037 - PfGW456L13_1286 0.13 -0.4 1,426,179 + PfGW456L13_1286 0.29 -0.8 1,426,179 + PfGW456L13_1286 0.29 -0.8 1,426,179 + PfGW456L13_1286 0.29 -1.2 1,426,179 + PfGW456L13_1286 0.29 -0.1 1,426,179 + PfGW456L13_1286 0.29 +1.0 1,426,180 - PfGW456L13_1286 0.29 +0.1 1,426,180 - PfGW456L13_1286 0.29 +0.3 1,426,180 - PfGW456L13_1286 0.29 +1.4 1,426,180 - PfGW456L13_1286 0.29 +1.5 1,426,180 - PfGW456L13_1286 0.29 -0.6 1,426,180 - PfGW456L13_1286 0.29 -0.4 1,426,213 + PfGW456L13_1286 0.33 -1.5 1,426,221 + PfGW456L13_1286 0.34 +0.4 1,426,221 + PfGW456L13_1286 0.34 -0.5 1,426,458 + PfGW456L13_1286 0.61 -0.5 1,426,458 + PfGW456L13_1286 0.61 +0.0 1,426,459 - PfGW456L13_1286 0.61 +0.6 1,426,536 - PfGW456L13_1286 0.70 +0.1 1,426,536 - PfGW456L13_1286 0.70 +0.9 1,426,536 - PfGW456L13_1286 0.70 +0.0 1,426,580 + PfGW456L13_1286 0.75 -0.3 1,426,581 - PfGW456L13_1286 0.75 +0.2 1,426,581 - PfGW456L13_1286 0.75 -0.1 1,426,581 - PfGW456L13_1286 0.75 +0.2 1,426,665 - PfGW456L13_1286 0.85 +0.6
Or see this region's nucleotide sequence