Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_1028

Experiment: KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_1026 and PfGW456L13_1027 overlap by 8 nucleotidesPfGW456L13_1027 and PfGW456L13_1028 are separated by 232 nucleotidesPfGW456L13_1028 and PfGW456L13_1029 overlap by 1 nucleotidesPfGW456L13_1029 and PfGW456L13_1030 overlap by 1 nucleotides PfGW456L13_1026 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 1,136,052 to 1,142,072 _1026 PfGW456L13_1027 - Chemotaxis signal transduction protein, at 1,142,065 to 1,142,526 _1027 PfGW456L13_1028 - Malonate decarboxylase alpha subunit, at 1,142,759 to 1,144,429 _1028 PfGW456L13_1029 - Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25), at 1,144,429 to 1,145,304 _1029 PfGW456L13_1030 - Malonate decarboxylase delta subunit, at 1,145,304 to 1,145,603 _1030 Position (kb) 1142 1143 1144 1145Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1142.001 kb on + strandat 1142.016 kb on + strandat 1142.165 kb on - strand, within PfGW456L13_1027at 1142.207 kb on - strand, within PfGW456L13_1027at 1142.404 kb on + strand, within PfGW456L13_1027at 1142.404 kb on + strand, within PfGW456L13_1027at 1142.404 kb on + strand, within PfGW456L13_1027at 1142.404 kb on + strand, within PfGW456L13_1027at 1142.405 kb on - strand, within PfGW456L13_1027at 1142.721 kb on + strandat 1142.721 kb on + strandat 1142.722 kb on - strandat 1142.722 kb on - strandat 1142.722 kb on - strandat 1143.158 kb on + strand, within PfGW456L13_1028at 1143.158 kb on + strand, within PfGW456L13_1028at 1143.158 kb on + strand, within PfGW456L13_1028at 1143.158 kb on + strand, within PfGW456L13_1028at 1143.158 kb on + strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.159 kb on - strand, within PfGW456L13_1028at 1143.170 kb on + strand, within PfGW456L13_1028at 1143.170 kb on + strand, within PfGW456L13_1028at 1143.171 kb on - strand, within PfGW456L13_1028at 1143.248 kb on + strand, within PfGW456L13_1028at 1143.248 kb on + strand, within PfGW456L13_1028at 1143.248 kb on + strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.249 kb on - strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.419 kb on + strand, within PfGW456L13_1028at 1143.420 kb on - strand, within PfGW456L13_1028at 1143.420 kb on - strand, within PfGW456L13_1028at 1143.420 kb on - strand, within PfGW456L13_1028at 1143.420 kb on - strand, within PfGW456L13_1028at 1143.420 kb on - strand, within PfGW456L13_1028at 1143.422 kb on - strand, within PfGW456L13_1028at 1143.504 kb on - strand, within PfGW456L13_1028at 1143.581 kb on + strand, within PfGW456L13_1028at 1143.581 kb on + strand, within PfGW456L13_1028at 1143.582 kb on - strand, within PfGW456L13_1028at 1143.620 kb on - strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.719 kb on + strand, within PfGW456L13_1028at 1143.720 kb on - strand, within PfGW456L13_1028at 1143.720 kb on - strand, within PfGW456L13_1028at 1143.807 kb on - strand, within PfGW456L13_1028at 1144.128 kb on - strand, within PfGW456L13_1028at 1144.178 kb on + strand, within PfGW456L13_1028at 1144.178 kb on + strand, within PfGW456L13_1028at 1144.178 kb on + strand, within PfGW456L13_1028at 1144.178 kb on + strand, within PfGW456L13_1028at 1144.179 kb on - strand, within PfGW456L13_1028at 1144.179 kb on - strand, within PfGW456L13_1028at 1144.179 kb on - strand, within PfGW456L13_1028at 1144.397 kb on + strandat 1144.397 kb on + strandat 1144.397 kb on + strandat 1144.398 kb on - strandat 1144.398 kb on - strandat 1144.398 kb on - strandat 1144.427 kb on + strandat 1144.440 kb on + strandat 1144.440 kb on + strandat 1144.440 kb on + strandat 1144.441 kb on - strandat 1144.821 kb on + strand, within PfGW456L13_1029at 1144.912 kb on + strand, within PfGW456L13_1029at 1145.315 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with PTLB purified from Pseudomonas sp. FW300-N2F2 0.008 x
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1,142,001 + -1.4
1,142,016 + +0.5
1,142,165 - PfGW456L13_1027 0.22 +1.5
1,142,207 - PfGW456L13_1027 0.31 -0.8
1,142,404 + PfGW456L13_1027 0.73 +0.4
1,142,404 + PfGW456L13_1027 0.73 +0.5
1,142,404 + PfGW456L13_1027 0.73 +0.2
1,142,404 + PfGW456L13_1027 0.73 +0.1
1,142,405 - PfGW456L13_1027 0.74 +0.6
1,142,721 + +0.7
1,142,721 + +0.2
1,142,722 - +1.8
1,142,722 - +0.8
1,142,722 - +0.2
1,143,158 + PfGW456L13_1028 0.24 +0.0
1,143,158 + PfGW456L13_1028 0.24 +0.4
1,143,158 + PfGW456L13_1028 0.24 +1.0
1,143,158 + PfGW456L13_1028 0.24 +0.1
1,143,158 + PfGW456L13_1028 0.24 +0.0
1,143,159 - PfGW456L13_1028 0.24 +0.8
1,143,159 - PfGW456L13_1028 0.24 -1.2
1,143,159 - PfGW456L13_1028 0.24 -1.3
1,143,159 - PfGW456L13_1028 0.24 -0.9
1,143,159 - PfGW456L13_1028 0.24 +0.2
1,143,159 - PfGW456L13_1028 0.24 -0.4
1,143,159 - PfGW456L13_1028 0.24 +0.5
1,143,159 - PfGW456L13_1028 0.24 +0.7
1,143,159 - PfGW456L13_1028 0.24 -1.3
1,143,170 + PfGW456L13_1028 0.25 +0.5
1,143,170 + PfGW456L13_1028 0.25 -0.6
1,143,171 - PfGW456L13_1028 0.25 +0.6
1,143,248 + PfGW456L13_1028 0.29 +1.4
1,143,248 + PfGW456L13_1028 0.29 +0.7
1,143,248 + PfGW456L13_1028 0.29 -0.1
1,143,249 - PfGW456L13_1028 0.29 +0.1
1,143,249 - PfGW456L13_1028 0.29 +0.6
1,143,249 - PfGW456L13_1028 0.29 +0.6
1,143,249 - PfGW456L13_1028 0.29 -1.0
1,143,249 - PfGW456L13_1028 0.29 -3.2
1,143,249 - PfGW456L13_1028 0.29 -0.1
1,143,419 + PfGW456L13_1028 0.39 +0.7
1,143,419 + PfGW456L13_1028 0.39 +0.0
1,143,419 + PfGW456L13_1028 0.39 -0.1
1,143,419 + PfGW456L13_1028 0.39 +1.4
1,143,419 + PfGW456L13_1028 0.39 +0.3
1,143,419 + PfGW456L13_1028 0.39 -0.6
1,143,419 + PfGW456L13_1028 0.39 -0.5
1,143,419 + PfGW456L13_1028 0.39 +1.8
1,143,420 - PfGW456L13_1028 0.40 -1.8
1,143,420 - PfGW456L13_1028 0.40 +1.6
1,143,420 - PfGW456L13_1028 0.40 +0.4
1,143,420 - PfGW456L13_1028 0.40 -0.9
1,143,420 - PfGW456L13_1028 0.40 -0.3
1,143,422 - PfGW456L13_1028 0.40 +0.5
1,143,504 - PfGW456L13_1028 0.45 +0.8
1,143,581 + PfGW456L13_1028 0.49 -1.0
1,143,581 + PfGW456L13_1028 0.49 -0.7
1,143,582 - PfGW456L13_1028 0.49 -0.5
1,143,620 - PfGW456L13_1028 0.52 -0.1
1,143,719 + PfGW456L13_1028 0.57 -0.3
1,143,719 + PfGW456L13_1028 0.57 +0.9
1,143,719 + PfGW456L13_1028 0.57 -3.4
1,143,719 + PfGW456L13_1028 0.57 -1.1
1,143,719 + PfGW456L13_1028 0.57 +1.0
1,143,719 + PfGW456L13_1028 0.57 +0.8
1,143,720 - PfGW456L13_1028 0.58 +0.1
1,143,720 - PfGW456L13_1028 0.58 -0.5
1,143,807 - PfGW456L13_1028 0.63 +0.3
1,144,128 - PfGW456L13_1028 0.82 +0.9
1,144,178 + PfGW456L13_1028 0.85 +1.5
1,144,178 + PfGW456L13_1028 0.85 -0.2
1,144,178 + PfGW456L13_1028 0.85 +0.3
1,144,178 + PfGW456L13_1028 0.85 +1.5
1,144,179 - PfGW456L13_1028 0.85 +1.0
1,144,179 - PfGW456L13_1028 0.85 +0.2
1,144,179 - PfGW456L13_1028 0.85 +1.1
1,144,397 + +0.2
1,144,397 + -0.6
1,144,397 + +1.5
1,144,398 - -0.2
1,144,398 - -0.5
1,144,398 - -2.3
1,144,427 + -0.5
1,144,440 + -0.2
1,144,440 + +0.8
1,144,440 + -0.2
1,144,441 - +0.6
1,144,821 + PfGW456L13_1029 0.45 -2.0
1,144,912 + PfGW456L13_1029 0.55 +1.5
1,145,315 - -0.2

Or see this region's nucleotide sequence