Experiment: Lactitol 10mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0223 and Echvi_0224 are separated by 261 nucleotides Echvi_0224 and Echvi_0225 are separated by 144 nucleotides Echvi_0225 and Echvi_0226 are separated by 25 nucleotides
Echvi_0223: Echvi_0223 - Septum formation initiator., at 241,095 to 241,382
_0223
Echvi_0224: Echvi_0224 - hypothetical protein, at 241,644 to 242,017
_0224
Echvi_0225: Echvi_0225 - phosphopyruvate hydratase, at 242,162 to 243,439
_0225
Echvi_0226: Echvi_0226 - carbamoyl-phosphate synthase, small subunit, at 243,465 to 244,559
_0226
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-1
0
1
2 at 241.398 kb on - strand at 241.402 kb on - strand at 241.411 kb on - strand at 241.419 kb on + strand at 241.448 kb on + strand at 241.448 kb on + strand at 241.449 kb on - strand at 241.454 kb on - strand at 241.460 kb on + strand at 241.487 kb on + strand at 241.496 kb on + strand at 241.496 kb on + strand at 241.501 kb on - strand at 241.503 kb on + strand at 241.560 kb on + strand at 241.561 kb on - strand at 241.650 kb on + strand at 241.651 kb on - strand at 241.651 kb on - strand at 241.691 kb on + strand, within Echvi_0224 at 241.692 kb on - strand, within Echvi_0224 at 241.764 kb on - strand, within Echvi_0224 at 241.768 kb on - strand, within Echvi_0224 at 241.782 kb on - strand, within Echvi_0224 at 241.890 kb on + strand, within Echvi_0224 at 241.891 kb on - strand, within Echvi_0224 at 241.962 kb on + strand, within Echvi_0224 at 241.963 kb on - strand, within Echvi_0224 at 241.996 kb on - strand at 242.142 kb on + strand at 242.145 kb on + strand at 242.163 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.467 kb on - strand at 243.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactitol 10mM (C) remove 241,398 - -1.4 241,402 - -1.7 241,411 - -0.3 241,419 + -0.6 241,448 + +0.2 241,448 + -0.9 241,449 - -1.3 241,454 - +0.4 241,460 + +2.1 241,487 + +1.8 241,496 + +1.4 241,496 + +0.8 241,501 - +0.1 241,503 + +0.1 241,560 + +0.3 241,561 - -0.9 241,650 + +0.4 241,651 - -1.7 241,651 - +1.4 241,691 + Echvi_0224 0.13 +0.8 241,692 - Echvi_0224 0.13 +0.6 241,764 - Echvi_0224 0.32 +2.7 241,768 - Echvi_0224 0.33 -0.0 241,782 - Echvi_0224 0.37 +0.4 241,890 + Echvi_0224 0.66 -0.6 241,891 - Echvi_0224 0.66 +1.3 241,962 + Echvi_0224 0.85 -0.4 241,963 - Echvi_0224 0.85 +0.7 241,996 - +0.2 242,142 + -1.0 242,145 + +1.0 242,163 + +0.2 243,457 - +0.8 243,457 - -0.2 243,457 - +0.0 243,457 - +0.1 243,467 - -1.2 243,471 - -1.0
Or see this region's nucleotide sequence