Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5518 and Atu5519 are separated by 86 nucleotides Atu5519 and Atu5520 are separated by 48 nucleotides Atu5520 and Atu5521 are separated by 26 nucleotides Atu5521 and Atu5522 are separated by 72 nucleotides
Atu5518: Atu5518 - hypothetical protein, at 509,639 to 511,147
Atu5518
Atu5519: Atu5519 - hypothetical protein, at 511,234 to 511,539
Atu5519
Atu5520: Atu5520 - acyl-CoA dehydrogenase, at 511,588 to 512,835
Atu5520
Atu5521: Atu5521 - ABC transporter nucleotide binding/ATPase (branched chain amino acid), at 512,862 to 513,641
Atu5521
Atu5522: Atu5522 - hypothetical protein, at 513,714 to 515,120
Atu5522
Position (kb) 511
512
513 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 510.646 kb on - strand, within Atu5518 at 510.698 kb on + strand, within Atu5518 at 510.783 kb on + strand, within Atu5518 at 510.791 kb on - strand, within Atu5518 at 510.800 kb on + strand, within Atu5518 at 510.817 kb on - strand, within Atu5518 at 510.817 kb on - strand, within Atu5518 at 510.822 kb on - strand, within Atu5518 at 510.849 kb on + strand, within Atu5518 at 510.915 kb on + strand, within Atu5518 at 511.000 kb on - strand at 511.000 kb on - strand at 511.016 kb on - strand at 511.118 kb on + strand at 511.150 kb on + strand at 511.165 kb on - strand at 511.230 kb on + strand at 511.292 kb on + strand, within Atu5519 at 511.297 kb on + strand, within Atu5519 at 511.320 kb on - strand, within Atu5519 at 511.347 kb on + strand, within Atu5519 at 511.355 kb on - strand, within Atu5519 at 511.355 kb on - strand, within Atu5519 at 511.370 kb on - strand, within Atu5519 at 511.373 kb on - strand, within Atu5519 at 511.667 kb on - strand at 511.674 kb on + strand at 511.680 kb on + strand at 511.689 kb on - strand at 511.695 kb on - strand at 511.725 kb on + strand, within Atu5520 at 511.821 kb on - strand, within Atu5520 at 511.864 kb on - strand, within Atu5520 at 511.894 kb on - strand, within Atu5520 at 511.899 kb on - strand, within Atu5520 at 511.940 kb on + strand, within Atu5520 at 511.940 kb on + strand, within Atu5520 at 511.993 kb on - strand, within Atu5520 at 512.074 kb on - strand, within Atu5520 at 512.099 kb on + strand, within Atu5520 at 512.099 kb on + strand, within Atu5520 at 512.099 kb on + strand, within Atu5520 at 512.107 kb on - strand, within Atu5520 at 512.107 kb on - strand, within Atu5520 at 512.113 kb on - strand, within Atu5520 at 512.129 kb on + strand, within Atu5520 at 512.154 kb on + strand, within Atu5520 at 512.167 kb on + strand, within Atu5520 at 512.192 kb on - strand, within Atu5520 at 512.225 kb on - strand, within Atu5520 at 512.245 kb on - strand, within Atu5520 at 512.257 kb on + strand, within Atu5520 at 512.257 kb on + strand, within Atu5520 at 512.293 kb on + strand, within Atu5520 at 512.293 kb on + strand, within Atu5520 at 512.293 kb on + strand, within Atu5520 at 512.296 kb on - strand, within Atu5520 at 512.301 kb on - strand, within Atu5520 at 512.558 kb on + strand, within Atu5520 at 512.573 kb on + strand, within Atu5520 at 512.711 kb on + strand at 512.741 kb on + strand at 512.755 kb on - strand at 512.755 kb on - strand at 512.813 kb on + strand at 512.895 kb on - strand at 513.031 kb on + strand, within Atu5521 at 513.068 kb on - strand, within Atu5521 at 513.076 kb on - strand, within Atu5521 at 513.076 kb on - strand, within Atu5521 at 513.092 kb on + strand, within Atu5521 at 513.104 kb on + strand, within Atu5521 at 513.112 kb on - strand, within Atu5521 at 513.112 kb on - strand, within Atu5521 at 513.172 kb on + strand, within Atu5521 at 513.180 kb on - strand, within Atu5521 at 513.182 kb on + strand, within Atu5521 at 513.296 kb on + strand, within Atu5521 at 513.304 kb on - strand, within Atu5521 at 513.305 kb on - strand, within Atu5521 at 513.368 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.431 kb on + strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.439 kb on - strand, within Atu5521 at 513.596 kb on - strand at 513.621 kb on - strand at 513.650 kb on + strand at 513.745 kb on - strand at 513.745 kb on - strand at 513.829 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 510,646 - Atu5518 0.67 -0.0 510,698 + Atu5518 0.70 -0.8 510,783 + Atu5518 0.76 +2.6 510,791 - Atu5518 0.76 +1.5 510,800 + Atu5518 0.77 +0.1 510,817 - Atu5518 0.78 -0.2 510,817 - Atu5518 0.78 -0.4 510,822 - Atu5518 0.78 +0.4 510,849 + Atu5518 0.80 -0.7 510,915 + Atu5518 0.85 -1.9 511,000 - -1.8 511,000 - -0.8 511,016 - -1.6 511,118 + -0.3 511,150 + -0.6 511,165 - -0.5 511,230 + -1.1 511,292 + Atu5519 0.19 +0.9 511,297 + Atu5519 0.21 -0.2 511,320 - Atu5519 0.28 -0.2 511,347 + Atu5519 0.37 +0.4 511,355 - Atu5519 0.40 -0.2 511,355 - Atu5519 0.40 -1.5 511,370 - Atu5519 0.44 -0.7 511,373 - Atu5519 0.45 -0.0 511,667 - -3.7 511,674 + -0.4 511,680 + -0.2 511,689 - +0.2 511,695 - +0.6 511,725 + Atu5520 0.11 -3.9 511,821 - Atu5520 0.19 -2.9 511,864 - Atu5520 0.22 -0.4 511,894 - Atu5520 0.25 +0.1 511,899 - Atu5520 0.25 -0.2 511,940 + Atu5520 0.28 +1.0 511,940 + Atu5520 0.28 -1.0 511,993 - Atu5520 0.32 -1.0 512,074 - Atu5520 0.39 -1.1 512,099 + Atu5520 0.41 -0.3 512,099 + Atu5520 0.41 +0.4 512,099 + Atu5520 0.41 -0.8 512,107 - Atu5520 0.42 +0.2 512,107 - Atu5520 0.42 -0.3 512,113 - Atu5520 0.42 +0.5 512,129 + Atu5520 0.43 +1.1 512,154 + Atu5520 0.45 -0.0 512,167 + Atu5520 0.46 -0.3 512,192 - Atu5520 0.48 -1.5 512,225 - Atu5520 0.51 +0.3 512,245 - Atu5520 0.53 -0.7 512,257 + Atu5520 0.54 -0.8 512,257 + Atu5520 0.54 -0.9 512,293 + Atu5520 0.56 -1.4 512,293 + Atu5520 0.56 -0.0 512,293 + Atu5520 0.56 -0.5 512,296 - Atu5520 0.57 -0.5 512,301 - Atu5520 0.57 -0.5 512,558 + Atu5520 0.78 -1.3 512,573 + Atu5520 0.79 -0.1 512,711 + +0.7 512,741 + -0.3 512,755 - +1.6 512,755 - +0.0 512,813 + +0.7 512,895 - +0.1 513,031 + Atu5521 0.22 -0.6 513,068 - Atu5521 0.26 -1.2 513,076 - Atu5521 0.27 +0.0 513,076 - Atu5521 0.27 +0.8 513,092 + Atu5521 0.29 +0.7 513,104 + Atu5521 0.31 -1.3 513,112 - Atu5521 0.32 +0.2 513,112 - Atu5521 0.32 +0.5 513,172 + Atu5521 0.40 -1.7 513,180 - Atu5521 0.41 +0.1 513,182 + Atu5521 0.41 -0.2 513,296 + Atu5521 0.56 -0.7 513,304 - Atu5521 0.57 +0.2 513,305 - Atu5521 0.57 -1.1 513,368 + Atu5521 0.65 +0.1 513,431 + Atu5521 0.73 -0.7 513,431 + Atu5521 0.73 +0.7 513,431 + Atu5521 0.73 +1.7 513,439 - Atu5521 0.74 -0.2 513,439 - Atu5521 0.74 +0.2 513,439 - Atu5521 0.74 +0.1 513,439 - Atu5521 0.74 +0.4 513,596 - -1.2 513,621 - -0.1 513,650 + +0.3 513,745 - +0.3 513,745 - +0.1 513,829 + -0.7
Or see this region's nucleotide sequence