Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4208 and Atu4209 are separated by 67 nucleotides Atu4209 and Atu4210 overlap by 1 nucleotides Atu4210 and Atu4211 are separated by 5 nucleotides
Atu4208: Atu4208 - sugar ABC transporter substrate-binding protein, at 1,330,887 to 1,332,260
Atu4208
Atu4209: Atu4209 - sugar ABC transporter permease, at 1,332,328 to 1,333,239
Atu4209
Atu4210: Atu4210 - sugars ABC transporter permease, at 1,333,239 to 1,334,297
Atu4210
Atu4211: Atu4211 - sugar ABC transporter ATPase, at 1,334,303 to 1,335,415
Atu4211
Position (kb)
1333
1334
1335 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1332.284 kb on + strand at 1332.284 kb on + strand at 1332.298 kb on - strand at 1332.342 kb on + strand at 1332.350 kb on - strand at 1332.372 kb on + strand at 1332.380 kb on - strand at 1332.555 kb on + strand, within Atu4209 at 1332.558 kb on - strand, within Atu4209 at 1332.571 kb on + strand, within Atu4209 at 1332.617 kb on + strand, within Atu4209 at 1332.734 kb on + strand, within Atu4209 at 1332.735 kb on - strand, within Atu4209 at 1332.742 kb on - strand, within Atu4209 at 1332.770 kb on + strand, within Atu4209 at 1332.778 kb on - strand, within Atu4209 at 1332.815 kb on - strand, within Atu4209 at 1332.859 kb on + strand, within Atu4209 at 1332.886 kb on - strand, within Atu4209 at 1333.011 kb on + strand, within Atu4209 at 1333.027 kb on - strand, within Atu4209 at 1333.085 kb on + strand, within Atu4209 at 1333.127 kb on + strand, within Atu4209 at 1333.135 kb on - strand, within Atu4209 at 1333.145 kb on + strand, within Atu4209 at 1333.261 kb on + strand at 1333.269 kb on + strand at 1333.449 kb on - strand, within Atu4210 at 1333.510 kb on + strand, within Atu4210 at 1333.515 kb on + strand, within Atu4210 at 1333.545 kb on - strand, within Atu4210 at 1333.545 kb on - strand, within Atu4210 at 1333.638 kb on + strand, within Atu4210 at 1333.669 kb on - strand, within Atu4210 at 1333.671 kb on + strand, within Atu4210 at 1333.863 kb on + strand, within Atu4210 at 1333.866 kb on - strand, within Atu4210 at 1333.942 kb on - strand, within Atu4210 at 1333.978 kb on + strand, within Atu4210 at 1333.993 kb on - strand, within Atu4210 at 1334.044 kb on - strand, within Atu4210 at 1334.072 kb on - strand, within Atu4210 at 1334.082 kb on + strand, within Atu4210 at 1334.091 kb on + strand, within Atu4210 at 1334.151 kb on - strand, within Atu4210 at 1334.156 kb on - strand, within Atu4210 at 1334.168 kb on + strand, within Atu4210 at 1334.168 kb on + strand, within Atu4210 at 1334.245 kb on - strand at 1334.245 kb on - strand at 1334.246 kb on - strand at 1334.285 kb on + strand at 1334.342 kb on + strand at 1334.676 kb on - strand, within Atu4211 at 1334.676 kb on - strand, within Atu4211 at 1334.714 kb on + strand, within Atu4211 at 1334.729 kb on + strand, within Atu4211 at 1334.806 kb on + strand, within Atu4211 at 1334.806 kb on + strand, within Atu4211 at 1334.814 kb on - strand, within Atu4211 at 1334.814 kb on - strand, within Atu4211 at 1334.825 kb on + strand, within Atu4211 at 1334.825 kb on + strand, within Atu4211 at 1334.827 kb on + strand, within Atu4211 at 1334.827 kb on + strand, within Atu4211 at 1334.827 kb on + strand, within Atu4211 at 1334.835 kb on - strand, within Atu4211 at 1334.882 kb on - strand, within Atu4211 at 1334.906 kb on - strand, within Atu4211 at 1335.037 kb on + strand, within Atu4211 at 1335.072 kb on + strand, within Atu4211 at 1335.108 kb on + strand, within Atu4211 at 1335.111 kb on - strand, within Atu4211 at 1335.111 kb on - strand, within Atu4211 at 1335.139 kb on + strand, within Atu4211 at 1335.143 kb on - strand, within Atu4211 at 1335.205 kb on + strand, within Atu4211 at 1335.268 kb on - strand, within Atu4211 at 1335.281 kb on + strand, within Atu4211
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 1,332,284 + +1.2 1,332,284 + -0.6 1,332,298 - +0.5 1,332,342 + +0.8 1,332,350 - -0.7 1,332,372 + +1.2 1,332,380 - -2.2 1,332,555 + Atu4209 0.25 +0.1 1,332,558 - Atu4209 0.25 -1.6 1,332,571 + Atu4209 0.27 -2.2 1,332,617 + Atu4209 0.32 +0.6 1,332,734 + Atu4209 0.45 -0.4 1,332,735 - Atu4209 0.45 -0.1 1,332,742 - Atu4209 0.45 -0.5 1,332,770 + Atu4209 0.48 -0.1 1,332,778 - Atu4209 0.49 -0.6 1,332,815 - Atu4209 0.53 +0.3 1,332,859 + Atu4209 0.58 +0.7 1,332,886 - Atu4209 0.61 -1.1 1,333,011 + Atu4209 0.75 +0.2 1,333,027 - Atu4209 0.77 -0.4 1,333,085 + Atu4209 0.83 -0.0 1,333,127 + Atu4209 0.88 +1.3 1,333,135 - Atu4209 0.88 +0.1 1,333,145 + Atu4209 0.90 -1.4 1,333,261 + -0.1 1,333,269 + -0.5 1,333,449 - Atu4210 0.20 +1.0 1,333,510 + Atu4210 0.26 +1.5 1,333,515 + Atu4210 0.26 -0.0 1,333,545 - Atu4210 0.29 +2.0 1,333,545 - Atu4210 0.29 -1.8 1,333,638 + Atu4210 0.38 -0.7 1,333,669 - Atu4210 0.41 -0.9 1,333,671 + Atu4210 0.41 +2.0 1,333,863 + Atu4210 0.59 +2.1 1,333,866 - Atu4210 0.59 +0.4 1,333,942 - Atu4210 0.66 -0.5 1,333,978 + Atu4210 0.70 +0.0 1,333,993 - Atu4210 0.71 +1.4 1,334,044 - Atu4210 0.76 +0.3 1,334,072 - Atu4210 0.79 -1.1 1,334,082 + Atu4210 0.80 -1.1 1,334,091 + Atu4210 0.80 -1.7 1,334,151 - Atu4210 0.86 -0.3 1,334,156 - Atu4210 0.87 -0.1 1,334,168 + Atu4210 0.88 -1.9 1,334,168 + Atu4210 0.88 +1.4 1,334,245 - +0.2 1,334,245 - +0.1 1,334,246 - -0.0 1,334,285 + -0.1 1,334,342 + +1.0 1,334,676 - Atu4211 0.34 +0.3 1,334,676 - Atu4211 0.34 +0.4 1,334,714 + Atu4211 0.37 +1.0 1,334,729 + Atu4211 0.38 +0.9 1,334,806 + Atu4211 0.45 +0.9 1,334,806 + Atu4211 0.45 +0.2 1,334,814 - Atu4211 0.46 -0.2 1,334,814 - Atu4211 0.46 -0.3 1,334,825 + Atu4211 0.47 -0.1 1,334,825 + Atu4211 0.47 +0.2 1,334,827 + Atu4211 0.47 -0.1 1,334,827 + Atu4211 0.47 +2.5 1,334,827 + Atu4211 0.47 +0.2 1,334,835 - Atu4211 0.48 -1.3 1,334,882 - Atu4211 0.52 +1.3 1,334,906 - Atu4211 0.54 -0.2 1,335,037 + Atu4211 0.66 +0.5 1,335,072 + Atu4211 0.69 +0.3 1,335,108 + Atu4211 0.72 +0.4 1,335,111 - Atu4211 0.73 -0.4 1,335,111 - Atu4211 0.73 -1.0 1,335,139 + Atu4211 0.75 +0.9 1,335,143 - Atu4211 0.75 -1.4 1,335,205 + Atu4211 0.81 -1.8 1,335,268 - Atu4211 0.87 -0.8 1,335,281 + Atu4211 0.88 -0.1
Or see this region's nucleotide sequence