Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3827 and Atu3828 are separated by 50 nucleotides Atu3828 and Atu3829 are separated by 66 nucleotides Atu3829 and Atu3830 are separated by 90 nucleotides
Atu3827: Atu3827 - LysR family transcriptional regulator, at 922,844 to 923,731
Atu3827
Atu3828: Atu3828 - acetyltransferase, at 923,782 to 924,360
Atu3828
Atu3829: Atu3829 - LysR family transcriptional regulator, at 924,427 to 925,278
Atu3829
Atu3830: Atu3830 - MFS permease, at 925,369 to 926,625
Atu3830
Position (kb) 924
925
926 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 923.427 kb on + strand, within Atu3827 at 923.624 kb on + strand, within Atu3827 at 923.632 kb on - strand, within Atu3827 at 923.666 kb on - strand at 923.758 kb on + strand at 923.788 kb on + strand at 923.833 kb on - strand at 923.842 kb on + strand, within Atu3828 at 923.848 kb on - strand, within Atu3828 at 923.917 kb on + strand, within Atu3828 at 923.917 kb on + strand, within Atu3828 at 923.925 kb on - strand, within Atu3828 at 924.095 kb on + strand, within Atu3828 at 924.107 kb on - strand, within Atu3828 at 924.109 kb on + strand, within Atu3828 at 924.138 kb on + strand, within Atu3828 at 924.145 kb on + strand, within Atu3828 at 924.172 kb on + strand, within Atu3828 at 924.172 kb on - strand, within Atu3828 at 924.195 kb on + strand, within Atu3828 at 924.199 kb on - strand, within Atu3828 at 924.203 kb on - strand, within Atu3828 at 924.206 kb on + strand, within Atu3828 at 924.235 kb on + strand, within Atu3828 at 924.250 kb on - strand, within Atu3828 at 924.257 kb on - strand, within Atu3828 at 924.268 kb on - strand, within Atu3828 at 924.348 kb on - strand at 924.418 kb on - strand at 924.507 kb on - strand at 924.538 kb on + strand, within Atu3829 at 924.546 kb on - strand, within Atu3829 at 924.559 kb on + strand, within Atu3829 at 924.656 kb on - strand, within Atu3829 at 924.735 kb on + strand, within Atu3829 at 924.767 kb on + strand, within Atu3829 at 924.777 kb on + strand, within Atu3829 at 924.947 kb on - strand, within Atu3829 at 924.962 kb on + strand, within Atu3829 at 924.972 kb on + strand, within Atu3829 at 924.972 kb on + strand, within Atu3829 at 925.020 kb on + strand, within Atu3829 at 925.054 kb on - strand, within Atu3829 at 925.072 kb on + strand, within Atu3829 at 925.115 kb on - strand, within Atu3829 at 925.140 kb on + strand, within Atu3829 at 925.181 kb on + strand, within Atu3829 at 925.193 kb on + strand at 925.211 kb on - strand at 925.400 kb on + strand at 925.408 kb on - strand at 925.425 kb on + strand at 925.425 kb on + strand at 925.433 kb on - strand at 925.433 kb on - strand at 925.463 kb on + strand at 925.573 kb on + strand, within Atu3830 at 925.573 kb on + strand, within Atu3830 at 925.653 kb on - strand, within Atu3830 at 925.666 kb on + strand, within Atu3830 at 925.674 kb on - strand, within Atu3830 at 925.737 kb on + strand, within Atu3830 at 925.792 kb on - strand, within Atu3830 at 925.800 kb on + strand, within Atu3830 at 925.839 kb on + strand, within Atu3830 at 925.880 kb on - strand, within Atu3830 at 925.904 kb on - strand, within Atu3830 at 926.069 kb on + strand, within Atu3830 at 926.132 kb on + strand, within Atu3830 at 926.132 kb on + strand, within Atu3830 at 926.140 kb on - strand, within Atu3830 at 926.140 kb on - strand, within Atu3830 at 926.206 kb on + strand, within Atu3830 at 926.214 kb on - strand, within Atu3830 at 926.214 kb on - strand, within Atu3830 at 926.214 kb on - strand, within Atu3830 at 926.237 kb on - strand, within Atu3830 at 926.255 kb on + strand, within Atu3830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 923,427 + Atu3827 0.66 -2.7 923,624 + Atu3827 0.88 +0.4 923,632 - Atu3827 0.89 -0.0 923,666 - -0.2 923,758 + +0.0 923,788 + -1.6 923,833 - -0.4 923,842 + Atu3828 0.10 +0.9 923,848 - Atu3828 0.11 -1.1 923,917 + Atu3828 0.23 -0.0 923,917 + Atu3828 0.23 -0.8 923,925 - Atu3828 0.25 +0.0 924,095 + Atu3828 0.54 -2.2 924,107 - Atu3828 0.56 -0.4 924,109 + Atu3828 0.56 -0.0 924,138 + Atu3828 0.61 -0.3 924,145 + Atu3828 0.63 -0.7 924,172 + Atu3828 0.67 +1.8 924,172 - Atu3828 0.67 +1.7 924,195 + Atu3828 0.71 -0.1 924,199 - Atu3828 0.72 +3.0 924,203 - Atu3828 0.73 -1.1 924,206 + Atu3828 0.73 -0.1 924,235 + Atu3828 0.78 +1.8 924,250 - Atu3828 0.81 -0.6 924,257 - Atu3828 0.82 +1.2 924,268 - Atu3828 0.84 -3.0 924,348 - -0.6 924,418 - -1.0 924,507 - -0.2 924,538 + Atu3829 0.13 -1.6 924,546 - Atu3829 0.14 +1.2 924,559 + Atu3829 0.15 -0.6 924,656 - Atu3829 0.27 -1.7 924,735 + Atu3829 0.36 -3.2 924,767 + Atu3829 0.40 -0.2 924,777 + Atu3829 0.41 -3.4 924,947 - Atu3829 0.61 -1.9 924,962 + Atu3829 0.63 +0.1 924,972 + Atu3829 0.64 -1.4 924,972 + Atu3829 0.64 -2.6 925,020 + Atu3829 0.70 -0.9 925,054 - Atu3829 0.74 -2.3 925,072 + Atu3829 0.76 +0.8 925,115 - Atu3829 0.81 +0.5 925,140 + Atu3829 0.84 -3.1 925,181 + Atu3829 0.88 -0.5 925,193 + -1.9 925,211 - -0.4 925,400 + -0.0 925,408 - +1.0 925,425 + -1.3 925,425 + +0.2 925,433 - -0.2 925,433 - +1.7 925,463 + -2.4 925,573 + Atu3830 0.16 +0.1 925,573 + Atu3830 0.16 +0.2 925,653 - Atu3830 0.23 -2.6 925,666 + Atu3830 0.24 -0.4 925,674 - Atu3830 0.24 -0.6 925,737 + Atu3830 0.29 -0.1 925,792 - Atu3830 0.34 +0.3 925,800 + Atu3830 0.34 -0.5 925,839 + Atu3830 0.37 +0.6 925,880 - Atu3830 0.41 +0.1 925,904 - Atu3830 0.43 -0.3 926,069 + Atu3830 0.56 -0.7 926,132 + Atu3830 0.61 -0.2 926,132 + Atu3830 0.61 -0.5 926,140 - Atu3830 0.61 -1.8 926,140 - Atu3830 0.61 +0.6 926,206 + Atu3830 0.67 -1.1 926,214 - Atu3830 0.67 +0.3 926,214 - Atu3830 0.67 -0.1 926,214 - Atu3830 0.67 -1.1 926,237 - Atu3830 0.69 -0.6 926,255 + Atu3830 0.70 -2.1
Or see this region's nucleotide sequence