Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3205 and Atu3206 are separated by 121 nucleotides Atu3206 and Atu3207 are separated by 318 nucleotides
Atu3205: Atu3205 - hypothetical protein, at 217,813 to 218,607
Atu3205
Atu3206: Atu3206 - hypothetical protein, at 218,729 to 219,772
Atu3206
Atu3207: Atu3207 - diguanylate cyclase, at 220,091 to 222,085
Atu3207
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 217.793 kb on - strand at 217.844 kb on + strand at 217.849 kb on - strand at 217.852 kb on - strand at 217.874 kb on - strand at 217.935 kb on + strand, within Atu3205 at 217.935 kb on + strand, within Atu3205 at 217.935 kb on + strand, within Atu3205 at 217.938 kb on + strand, within Atu3205 at 217.943 kb on - strand, within Atu3205 at 217.948 kb on - strand, within Atu3205 at 217.983 kb on + strand, within Atu3205 at 218.030 kb on - strand, within Atu3205 at 218.051 kb on + strand, within Atu3205 at 218.224 kb on + strand, within Atu3205 at 218.325 kb on + strand, within Atu3205 at 218.470 kb on - strand, within Atu3205 at 218.499 kb on - strand, within Atu3205 at 218.499 kb on - strand, within Atu3205 at 218.508 kb on + strand, within Atu3205 at 218.536 kb on - strand at 218.536 kb on - strand at 218.601 kb on - strand at 218.602 kb on + strand at 218.602 kb on + strand at 218.615 kb on + strand at 218.673 kb on - strand at 218.683 kb on + strand at 218.718 kb on + strand at 218.726 kb on + strand at 218.785 kb on + strand at 218.889 kb on - strand, within Atu3206 at 218.905 kb on + strand, within Atu3206 at 218.950 kb on + strand, within Atu3206 at 218.955 kb on + strand, within Atu3206 at 218.995 kb on - strand, within Atu3206 at 218.998 kb on - strand, within Atu3206 at 219.002 kb on + strand, within Atu3206 at 219.023 kb on - strand, within Atu3206 at 219.038 kb on - strand, within Atu3206 at 219.053 kb on - strand, within Atu3206 at 219.055 kb on + strand, within Atu3206 at 219.055 kb on + strand, within Atu3206 at 219.058 kb on + strand, within Atu3206 at 219.063 kb on - strand, within Atu3206 at 219.063 kb on - strand, within Atu3206 at 219.129 kb on - strand, within Atu3206 at 219.136 kb on + strand, within Atu3206 at 219.144 kb on - strand, within Atu3206 at 219.153 kb on + strand, within Atu3206 at 219.308 kb on + strand, within Atu3206 at 219.326 kb on - strand, within Atu3206 at 219.358 kb on + strand, within Atu3206 at 219.366 kb on - strand, within Atu3206 at 219.366 kb on - strand, within Atu3206 at 219.366 kb on - strand, within Atu3206 at 219.371 kb on - strand, within Atu3206 at 219.384 kb on - strand, within Atu3206 at 219.470 kb on - strand, within Atu3206 at 219.495 kb on + strand, within Atu3206 at 219.721 kb on + strand at 219.731 kb on + strand at 219.854 kb on + strand at 219.902 kb on + strand at 219.989 kb on + strand at 219.989 kb on + strand at 220.164 kb on + strand at 220.228 kb on - strand at 220.270 kb on - strand at 220.330 kb on - strand, within Atu3207 at 220.355 kb on - strand, within Atu3207 at 220.357 kb on + strand, within Atu3207 at 220.380 kb on + strand, within Atu3207 at 220.380 kb on + strand, within Atu3207 at 220.388 kb on - strand, within Atu3207 at 220.388 kb on - strand, within Atu3207 at 220.388 kb on - strand, within Atu3207 at 220.388 kb on - strand, within Atu3207 at 220.408 kb on + strand, within Atu3207 at 220.484 kb on - strand, within Atu3207 at 220.489 kb on - strand, within Atu3207 at 220.530 kb on + strand, within Atu3207 at 220.532 kb on - strand, within Atu3207 at 220.660 kb on - strand, within Atu3207 at 220.730 kb on + strand, within Atu3207 at 220.740 kb on - strand, within Atu3207 at 220.740 kb on - strand, within Atu3207
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 217,793 - +0.7 217,844 + -2.9 217,849 - -0.0 217,852 - -0.5 217,874 - +0.3 217,935 + Atu3205 0.15 +1.3 217,935 + Atu3205 0.15 -1.3 217,935 + Atu3205 0.15 -1.5 217,938 + Atu3205 0.16 +0.9 217,943 - Atu3205 0.16 +0.3 217,948 - Atu3205 0.17 +0.7 217,983 + Atu3205 0.21 -1.4 218,030 - Atu3205 0.27 -0.4 218,051 + Atu3205 0.30 +2.2 218,224 + Atu3205 0.52 +0.2 218,325 + Atu3205 0.64 +0.9 218,470 - Atu3205 0.83 -2.0 218,499 - Atu3205 0.86 -2.7 218,499 - Atu3205 0.86 -0.1 218,508 + Atu3205 0.87 -0.5 218,536 - +0.1 218,536 - -0.6 218,601 - -2.3 218,602 + -1.9 218,602 + -0.4 218,615 + +0.1 218,673 - +0.5 218,683 + +1.3 218,718 + +0.1 218,726 + -0.8 218,785 + -0.9 218,889 - Atu3206 0.15 -1.1 218,905 + Atu3206 0.17 -0.4 218,950 + Atu3206 0.21 -0.4 218,955 + Atu3206 0.22 -0.3 218,995 - Atu3206 0.25 -0.3 218,998 - Atu3206 0.26 +0.8 219,002 + Atu3206 0.26 -0.9 219,023 - Atu3206 0.28 +0.1 219,038 - Atu3206 0.30 -0.6 219,053 - Atu3206 0.31 -2.0 219,055 + Atu3206 0.31 +0.7 219,055 + Atu3206 0.31 +1.0 219,058 + Atu3206 0.32 -1.5 219,063 - Atu3206 0.32 +0.2 219,063 - Atu3206 0.32 +0.2 219,129 - Atu3206 0.38 -0.9 219,136 + Atu3206 0.39 +0.7 219,144 - Atu3206 0.40 -0.4 219,153 + Atu3206 0.41 -0.7 219,308 + Atu3206 0.55 -0.6 219,326 - Atu3206 0.57 -0.1 219,358 + Atu3206 0.60 -0.5 219,366 - Atu3206 0.61 -0.8 219,366 - Atu3206 0.61 -0.2 219,366 - Atu3206 0.61 -0.4 219,371 - Atu3206 0.61 -0.2 219,384 - Atu3206 0.63 +0.4 219,470 - Atu3206 0.71 -0.6 219,495 + Atu3206 0.73 -1.5 219,721 + -1.0 219,731 + -1.3 219,854 + -0.1 219,902 + +0.6 219,989 + +0.8 219,989 + +0.4 220,164 + +0.2 220,228 - -1.9 220,270 - -1.5 220,330 - Atu3207 0.12 -0.8 220,355 - Atu3207 0.13 +0.1 220,357 + Atu3207 0.13 +0.6 220,380 + Atu3207 0.14 -1.7 220,380 + Atu3207 0.14 -1.8 220,388 - Atu3207 0.15 +0.4 220,388 - Atu3207 0.15 +0.5 220,388 - Atu3207 0.15 +0.3 220,388 - Atu3207 0.15 -2.5 220,408 + Atu3207 0.16 -1.5 220,484 - Atu3207 0.20 +0.0 220,489 - Atu3207 0.20 -0.4 220,530 + Atu3207 0.22 -0.3 220,532 - Atu3207 0.22 +0.7 220,660 - Atu3207 0.29 +0.6 220,730 + Atu3207 0.32 -0.4 220,740 - Atu3207 0.33 -1.3 220,740 - Atu3207 0.33 +0.8
Or see this region's nucleotide sequence