Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1650 and Atu1652 are separated by 654 nucleotides Atu1652 and Atu1653 are separated by 165 nucleotides
Atu1650: Atu1650 - acyltransferase, at 1,634,139 to 1,635,263
Atu1650
Atu1652: Atu1652 - NADP-dependent malic enzyme, at 1,635,918 to 1,638,233
Atu1652
Atu1653: Atu1653 - hypothetical protein, at 1,638,399 to 1,639,484
Atu1653
Position (kb)
1635
1636
1637
1638
1639 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1634.963 kb on - strand, within Atu1650 at 1634.986 kb on + strand, within Atu1650 at 1634.986 kb on + strand, within Atu1650 at 1635.129 kb on - strand, within Atu1650 at 1635.225 kb on + strand at 1635.240 kb on + strand at 1635.240 kb on + strand at 1635.241 kb on - strand at 1635.252 kb on + strand at 1635.256 kb on - strand at 1635.260 kb on - strand at 1635.641 kb on + strand at 1635.641 kb on + strand at 1635.646 kb on + strand at 1635.678 kb on - strand at 1635.678 kb on - strand at 1635.741 kb on + strand at 1635.768 kb on - strand at 1635.806 kb on + strand at 1635.807 kb on + strand at 1635.807 kb on + strand at 1635.874 kb on + strand at 1635.874 kb on + strand at 1638.260 kb on - strand at 1638.449 kb on - strand at 1638.451 kb on - strand at 1638.454 kb on - strand at 1638.457 kb on - strand at 1638.471 kb on - strand at 1638.502 kb on - strand at 1638.515 kb on + strand, within Atu1653 at 1638.609 kb on - strand, within Atu1653 at 1638.782 kb on + strand, within Atu1653 at 1638.906 kb on - strand, within Atu1653 at 1638.954 kb on + strand, within Atu1653 at 1639.096 kb on - strand, within Atu1653
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 1,634,963 - Atu1650 0.73 -1.6 1,634,986 + Atu1650 0.75 -3.9 1,634,986 + Atu1650 0.75 -0.6 1,635,129 - Atu1650 0.88 +0.6 1,635,225 + -1.0 1,635,240 + -0.7 1,635,240 + +0.3 1,635,241 - -0.5 1,635,252 + +0.9 1,635,256 - +0.4 1,635,260 - +0.4 1,635,641 + +0.1 1,635,641 + -0.8 1,635,646 + -1.5 1,635,678 - -1.8 1,635,678 - +0.5 1,635,741 + -0.1 1,635,768 - -0.1 1,635,806 + -1.9 1,635,807 + +1.5 1,635,807 + -1.0 1,635,874 + -0.1 1,635,874 + -1.5 1,638,260 - -1.3 1,638,449 - -0.2 1,638,451 - +1.7 1,638,454 - +0.2 1,638,457 - -0.4 1,638,471 - -0.9 1,638,502 - +0.1 1,638,515 + Atu1653 0.11 -1.2 1,638,609 - Atu1653 0.19 +2.3 1,638,782 + Atu1653 0.35 -2.3 1,638,906 - Atu1653 0.47 +0.1 1,638,954 + Atu1653 0.51 +1.3 1,639,096 - Atu1653 0.64 -1.2
Or see this region's nucleotide sequence