Experiment: R2A with tungstate 375 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1131 and Atu1132 are separated by 117 nucleotides Atu1132 and Atu1133 are separated by 31 nucleotides Atu1133 and Atu1134 are separated by 198 nucleotides
Atu1131: Atu1131 - outer membrane protein, at 1,120,603 to 1,121,259
Atu1131
Atu1132: Atu1132 - glutathione S-transferase related protein, at 1,121,377 to 1,121,973
Atu1132
Atu1133: Atu1133 - ribonuclease, at 1,122,005 to 1,122,763
Atu1133
Atu1134: Atu1134 - hypothetical protein, at 1,122,962 to 1,123,909
Atu1134
Position (kb)
1121
1122 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1120.462 kb on - strand at 1120.574 kb on + strand at 1120.623 kb on + strand at 1120.687 kb on - strand, within Atu1131 at 1120.709 kb on + strand, within Atu1131 at 1120.709 kb on + strand, within Atu1131 at 1120.740 kb on + strand, within Atu1131 at 1120.742 kb on - strand, within Atu1131 at 1120.745 kb on + strand, within Atu1131 at 1120.851 kb on - strand, within Atu1131 at 1120.896 kb on - strand, within Atu1131 at 1120.946 kb on + strand, within Atu1131 at 1120.948 kb on + strand, within Atu1131 at 1121.027 kb on + strand, within Atu1131 at 1121.035 kb on + strand, within Atu1131 at 1121.083 kb on + strand, within Atu1131 at 1121.091 kb on - strand, within Atu1131 at 1121.091 kb on - strand, within Atu1131 at 1121.116 kb on + strand, within Atu1131 at 1121.355 kb on - strand at 1121.382 kb on - strand at 1121.382 kb on - strand at 1121.382 kb on - strand at 1121.426 kb on + strand at 1121.456 kb on - strand, within Atu1132 at 1121.484 kb on + strand, within Atu1132 at 1121.558 kb on + strand, within Atu1132 at 1121.566 kb on - strand, within Atu1132 at 1121.576 kb on - strand, within Atu1132 at 1121.735 kb on + strand, within Atu1132 at 1121.735 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.788 kb on + strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.796 kb on - strand, within Atu1132 at 1121.870 kb on + strand, within Atu1132 at 1121.894 kb on - strand, within Atu1132 at 1121.906 kb on - strand, within Atu1132 at 1121.939 kb on - strand at 1121.944 kb on + strand at 1121.947 kb on - strand at 1122.044 kb on + strand at 1122.058 kb on + strand at 1122.134 kb on + strand, within Atu1133 at 1122.157 kb on + strand, within Atu1133 at 1122.173 kb on + strand, within Atu1133 at 1122.193 kb on + strand, within Atu1133 at 1122.207 kb on + strand, within Atu1133 at 1122.215 kb on - strand, within Atu1133 at 1122.215 kb on - strand, within Atu1133 at 1122.306 kb on - strand, within Atu1133 at 1122.306 kb on - strand, within Atu1133 at 1122.306 kb on - strand, within Atu1133 at 1122.306 kb on - strand, within Atu1133 at 1122.316 kb on + strand, within Atu1133 at 1122.460 kb on + strand, within Atu1133 at 1122.475 kb on - strand, within Atu1133 at 1122.519 kb on - strand, within Atu1133 at 1122.622 kb on + strand, within Atu1133 at 1122.702 kb on + strand at 1122.793 kb on + strand at 1122.815 kb on + strand at 1122.890 kb on - strand at 1122.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with tungstate 375 mM remove 1,120,462 - +0.9 1,120,574 + +1.0 1,120,623 + -0.3 1,120,687 - Atu1131 0.13 -1.0 1,120,709 + Atu1131 0.16 +0.1 1,120,709 + Atu1131 0.16 -0.3 1,120,740 + Atu1131 0.21 +0.3 1,120,742 - Atu1131 0.21 +0.1 1,120,745 + Atu1131 0.22 -0.3 1,120,851 - Atu1131 0.38 -2.3 1,120,896 - Atu1131 0.45 +2.0 1,120,946 + Atu1131 0.52 -2.2 1,120,948 + Atu1131 0.53 +0.5 1,121,027 + Atu1131 0.65 -0.2 1,121,035 + Atu1131 0.66 -2.2 1,121,083 + Atu1131 0.73 +1.8 1,121,091 - Atu1131 0.74 -1.3 1,121,091 - Atu1131 0.74 -2.8 1,121,116 + Atu1131 0.78 +0.2 1,121,355 - -0.1 1,121,382 - +2.8 1,121,382 - +0.3 1,121,382 - +1.0 1,121,426 + +0.9 1,121,456 - Atu1132 0.13 -0.9 1,121,484 + Atu1132 0.18 -0.4 1,121,558 + Atu1132 0.30 -2.6 1,121,566 - Atu1132 0.32 -2.6 1,121,576 - Atu1132 0.33 -0.4 1,121,735 + Atu1132 0.60 -0.5 1,121,735 + Atu1132 0.60 +0.7 1,121,788 + Atu1132 0.69 -0.3 1,121,788 + Atu1132 0.69 -2.3 1,121,788 + Atu1132 0.69 -1.4 1,121,788 + Atu1132 0.69 -2.6 1,121,788 + Atu1132 0.69 -2.3 1,121,788 + Atu1132 0.69 -1.1 1,121,788 + Atu1132 0.69 -1.7 1,121,788 + Atu1132 0.69 +1.2 1,121,788 + Atu1132 0.69 -0.2 1,121,788 + Atu1132 0.69 +0.1 1,121,788 + Atu1132 0.69 -1.6 1,121,796 - Atu1132 0.70 -0.7 1,121,796 - Atu1132 0.70 +0.1 1,121,796 - Atu1132 0.70 -0.6 1,121,796 - Atu1132 0.70 -1.0 1,121,796 - Atu1132 0.70 +1.2 1,121,796 - Atu1132 0.70 -0.8 1,121,796 - Atu1132 0.70 -1.1 1,121,796 - Atu1132 0.70 -0.0 1,121,870 + Atu1132 0.83 -0.1 1,121,894 - Atu1132 0.87 -0.2 1,121,906 - Atu1132 0.89 +0.0 1,121,939 - +1.5 1,121,944 + -0.6 1,121,947 - +0.3 1,122,044 + +0.8 1,122,058 + -0.9 1,122,134 + Atu1133 0.17 +0.0 1,122,157 + Atu1133 0.20 +0.8 1,122,173 + Atu1133 0.22 -0.3 1,122,193 + Atu1133 0.25 +0.4 1,122,207 + Atu1133 0.27 -0.7 1,122,215 - Atu1133 0.28 -3.5 1,122,215 - Atu1133 0.28 -0.3 1,122,306 - Atu1133 0.40 +0.0 1,122,306 - Atu1133 0.40 -1.8 1,122,306 - Atu1133 0.40 -1.3 1,122,306 - Atu1133 0.40 -2.9 1,122,316 + Atu1133 0.41 +1.4 1,122,460 + Atu1133 0.60 +0.4 1,122,475 - Atu1133 0.62 +0.7 1,122,519 - Atu1133 0.68 -0.1 1,122,622 + Atu1133 0.81 -1.0 1,122,702 + +0.2 1,122,793 + -0.0 1,122,815 + -0.5 1,122,890 - +1.3 1,122,902 - -0.8
Or see this region's nucleotide sequence