Strain Fitness in Escherichia coli BW25113 around b4004

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
4,200,378	+	zraS	b4003	0.31	-2.1
4,200,398	-	zraS	b4003	0.32	-0.4
4,200,431	+	zraS	b4003	0.34	+0.3
4,200,441	-	zraS	b4003	0.35	-1.8
4,200,441	-	zraS	b4003	0.35	+2.1
4,200,525	+	zraS	b4003	0.41	+0.3
4,200,525	+	zraS	b4003	0.41	-0.2
4,200,530	+	zraS	b4003	0.42	+1.1
4,200,532	+	zraS	b4003	0.42	-3.0
4,200,533	-	zraS	b4003	0.42	+0.3
4,200,586	-	zraS	b4003	0.46	-1.9
4,200,647	-	zraS	b4003	0.50	-1.0
4,200,647	-	zraS	b4003	0.50	-1.8
4,200,755	-	zraS	b4003	0.58	+1.3
4,200,757	-	zraS	b4003	0.58	+1.8
4,200,801	-	zraS	b4003	0.61	-0.3
4,200,902	-	zraS	b4003	0.68	+1.4
4,200,965	-	zraS	b4003	0.73	-1.2
4,200,965	-	zraS	b4003	0.73	-0.5
4,201,008	-	zraS	b4003	0.76	+2.8
4,201,008	-	zraS	b4003	0.76	+0.3
4,201,013	-	zraS	b4003	0.76	+1.1
4,201,013	-	zraS	b4003	0.76	+0.2
4,201,013	-	zraS	b4003	0.76	-0.4
4,201,023	-	zraS	b4003	0.77	+0.7
4,201,023	-	zraS	b4003	0.77	-1.6
4,201,028	-	zraS	b4003	0.77	+0.8
4,201,051	-	zraS	b4003	0.79	+3.1
4,201,150	+	zraS	b4003	0.86	-2.3
4,201,150	+	zraS	b4003	0.86	+1.1
4,201,250	+				-0.5
4,201,308	-				+0.3
4,201,313	+				-1.6
4,201,313	+				-0.4
4,201,362	+				-0.9
4,201,409	+				+1.2
4,201,428	-				+0.3
4,201,509	+	zraR	b4004	0.13	-1.4
4,201,593	-	zraR	b4004	0.19	-1.5
4,201,593	-	zraR	b4004	0.19	-0.6
4,201,600	+	zraR	b4004	0.19	+0.2
4,201,600	+	zraR	b4004	0.19	-0.9
4,201,640	-	zraR	b4004	0.22	-0.0
4,201,685	-	zraR	b4004	0.26	+2.1
4,201,707	+	zraR	b4004	0.27	-0.6
4,201,717	+	zraR	b4004	0.28	-0.4
4,201,719	+	zraR	b4004	0.28	+0.1
4,201,722	-	zraR	b4004	0.29	+0.1
4,201,722	-	zraR	b4004	0.29	+1.8
4,201,727	-	zraR	b4004	0.29	-1.8
4,201,727	-	zraR	b4004	0.29	-0.9
4,201,727	-	zraR	b4004	0.29	-0.6
4,201,746	+	zraR	b4004	0.30	-1.7
4,201,756	+	zraR	b4004	0.31	+0.2
4,201,789	-	zraR	b4004	0.34	+0.4
4,201,798	+	zraR	b4004	0.34	+0.1
4,201,798	+	zraR	b4004	0.34	-0.1
4,201,841	+	zraR	b4004	0.38	+0.5
4,201,873	+	zraR	b4004	0.40	-2.0
4,201,932	+	zraR	b4004	0.44	+0.1
4,201,935	-	zraR	b4004	0.45	+0.1
4,201,935	-	zraR	b4004	0.45	-0.9
4,201,945	+	zraR	b4004	0.45	-1.4
4,202,109	+	zraR	b4004	0.58	-0.4
4,202,111	+	zraR	b4004	0.58	+0.2
4,202,195	-	zraR	b4004	0.64	+0.1
4,202,199	+	zraR	b4004	0.65	-0.5
4,202,214	-	zraR	b4004	0.66	+0.2
4,202,239	-	zraR	b4004	0.68	+0.6
4,202,239	-	zraR	b4004	0.68	-1.9
4,202,420	-	zraR	b4004	0.81	-1.8
4,202,420	-	zraR	b4004	0.81	-0.9
4,202,631	-				-0.4
4,202,758	+				+0.6
4,202,777	-				-0.5
4,202,783	-				-0.0
4,202,785	-				+1.3
4,202,785	-				-0.0
4,202,800	+	purD	b4005	0.10	-0.1
4,202,852	-	purD	b4005	0.14	-0.4
4,202,865	+	purD	b4005	0.16	-2.1
4,202,904	+	purD	b4005	0.19	-0.1
4,202,904	+	purD	b4005	0.19	-0.7
4,202,905	-	purD	b4005	0.19	-0.2
4,202,905	-	purD	b4005	0.19	-0.3
4,202,993	-	purD	b4005	0.25	-2.0
4,203,007	+	purD	b4005	0.27	+0.3
4,203,030	+	purD	b4005	0.28	+0.5
4,203,128	+	purD	b4005	0.36	-2.9
4,203,128	+	purD	b4005	0.36	+1.1
4,203,130	+	purD	b4005	0.36	-0.2
4,203,143	-	purD	b4005	0.37	+0.5
4,203,159	+	purD	b4005	0.38	+1.5
4,203,235	+	purD	b4005	0.44	+1.6
4,203,243	-	purD	b4005	0.45	-0.9
4,203,360	+	purD	b4005	0.54	+0.1
4,203,385	+	purD	b4005	0.56	+0.4
4,203,428	+	purD	b4005	0.59	-0.2
4,203,445	+	purD	b4005	0.60	-0.9
4,203,445	+	purD	b4005	0.60	+0.1
4,203,491	-	purD	b4005	0.64	-2.2
4,203,491	-	purD	b4005	0.64	-0.7
4,203,496	+	purD	b4005	0.64	-0.8
4,203,505	+	purD	b4005	0.65	+1.3
4,203,550	-	purD	b4005	0.69	-0.5
4,203,657	-	purD	b4005	0.77	+0.6
4,203,657	-	purD	b4005	0.77	+1.2