Strain Fitness in Escherichia coli BW25113 around b3973

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurB and birA overlap by 4 nucleotidesbirA and coaA are separated by 28 nucleotides b3972: murB - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI), at 4,170,080 to 4,171,108 murB b3973: birA - biotin--protein ligase (NCBI), at 4,171,105 to 4,172,070 birA b3974: coaA - pantothenate kinase (NCBI), at 4,172,099 to 4,173,049 coaA Position (kb) 4171 4172 4173Strain fitness (log2 ratio) -1 0 1at 4171.110 kb on + strandat 4171.236 kb on + strand, within birAat 4171.284 kb on + strand, within birAat 4172.112 kb on - strandat 4172.981 kb on - strandat 4172.981 kb on - strandat 4173.047 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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4,171,110 + -0.2
4,171,236 + birA b3973 0.14 -0.4
4,171,284 + birA b3973 0.19 -1.3
4,172,112 - +1.5
4,172,981 - -1.3
4,172,981 - +0.2
4,173,047 - +0.4

Or see this region's nucleotide sequence