Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pflD and pflC overlap by 35 nucleotides pflC and frwD are separated by 1 nucleotides frwD and yijO overlap by 14 nucleotides
b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315
pflD
b3952: pflC - probable pyruvate formate lyase activating enzyme 2 (VIMSS), at 4,144,281 to 4,145,159
pflC
b3953: frwD - predicted enzyme IIB component of PTS (NCBI), at 4,145,161 to 4,145,502
frwD
b3954: yijO - predicted DNA-binding transcriptional regulator (NCBI), at 4,145,489 to 4,146,340
yijO
Position (kb)
4145
4146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4144.162 kb on - strand at 4144.235 kb on + strand at 4144.235 kb on + strand at 4144.243 kb on - strand at 4144.243 kb on - strand at 4144.287 kb on - strand at 4144.304 kb on - strand at 4144.317 kb on - strand at 4144.317 kb on - strand at 4144.409 kb on + strand, within pflC at 4144.445 kb on - strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.454 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.479 kb on + strand, within pflC at 4144.505 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.592 kb on - strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.599 kb on + strand, within pflC at 4144.709 kb on + strand, within pflC at 4144.770 kb on - strand, within pflC at 4144.841 kb on + strand, within pflC at 4144.952 kb on + strand, within pflC at 4144.983 kb on - strand, within pflC at 4144.983 kb on - strand, within pflC at 4145.095 kb on - strand at 4145.097 kb on + strand at 4145.097 kb on + strand at 4145.109 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.142 kb on - strand at 4145.190 kb on + strand at 4145.190 kb on + strand at 4145.261 kb on - strand, within frwD at 4145.263 kb on - strand, within frwD at 4145.264 kb on - strand, within frwD at 4145.341 kb on - strand, within frwD at 4145.354 kb on + strand, within frwD at 4145.354 kb on + strand, within frwD at 4145.364 kb on + strand, within frwD at 4145.364 kb on + strand, within frwD at 4145.372 kb on - strand, within frwD at 4145.398 kb on + strand, within frwD at 4145.398 kb on + strand, within frwD at 4145.411 kb on - strand, within frwD at 4145.419 kb on + strand, within frwD at 4145.452 kb on + strand, within frwD at 4145.499 kb on - strand at 4145.500 kb on - strand at 4145.525 kb on + strand at 4145.612 kb on + strand, within yijO at 4145.626 kb on - strand, within yijO at 4145.638 kb on + strand, within yijO at 4145.658 kb on + strand, within yijO at 4145.658 kb on + strand, within yijO at 4145.669 kb on + strand, within yijO at 4145.669 kb on + strand, within yijO at 4145.677 kb on - strand, within yijO at 4145.697 kb on - strand, within yijO at 4145.697 kb on - strand, within yijO at 4145.800 kb on - strand, within yijO at 4145.843 kb on + strand, within yijO at 4145.853 kb on + strand, within yijO at 4145.856 kb on - strand, within yijO at 4145.900 kb on + strand, within yijO at 4145.900 kb on + strand, within yijO at 4146.038 kb on + strand, within yijO at 4146.069 kb on + strand, within yijO at 4146.091 kb on - strand, within yijO at 4146.093 kb on + strand, within yijO at 4146.137 kb on + strand, within yijO at 4146.140 kb on + strand, within yijO at 4146.148 kb on + strand, within yijO at 4146.162 kb on + strand, within yijO at 4146.162 kb on + strand, within yijO at 4146.168 kb on + strand, within yijO at 4146.174 kb on + strand, within yijO at 4146.179 kb on + strand, within yijO at 4146.207 kb on + strand, within yijO at 4146.238 kb on - strand, within yijO at 4146.242 kb on + strand, within yijO at 4146.290 kb on + strand at 4146.338 kb on - strand at 4146.347 kb on - strand at 4146.359 kb on - strand at 4146.419 kb on - strand at 4146.457 kb on + strand at 4146.458 kb on - strand at 4146.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 4,144,162 - -2.1 4,144,235 + +1.9 4,144,235 + +0.6 4,144,243 - +0.4 4,144,243 - -0.3 4,144,287 - -1.4 4,144,304 - -1.4 4,144,317 - +0.1 4,144,317 - +0.9 4,144,409 + pflC b3952 0.15 +1.4 4,144,445 - pflC b3952 0.19 -0.0 4,144,454 + pflC b3952 0.20 -0.1 4,144,454 + pflC b3952 0.20 +1.1 4,144,479 + pflC b3952 0.23 +0.1 4,144,479 + pflC b3952 0.23 +2.2 4,144,505 - pflC b3952 0.25 -0.5 4,144,592 - pflC b3952 0.35 +0.9 4,144,592 - pflC b3952 0.35 +1.5 4,144,599 + pflC b3952 0.36 +0.8 4,144,599 + pflC b3952 0.36 +0.1 4,144,709 + pflC b3952 0.49 +0.6 4,144,770 - pflC b3952 0.56 +1.4 4,144,841 + pflC b3952 0.64 +0.2 4,144,952 + pflC b3952 0.76 -0.1 4,144,983 - pflC b3952 0.80 +0.7 4,144,983 - pflC b3952 0.80 +1.4 4,145,095 - -1.2 4,145,097 + -1.1 4,145,097 + -0.5 4,145,109 - +0.9 4,145,142 - -0.6 4,145,142 - +0.4 4,145,142 - -1.0 4,145,190 + +0.1 4,145,190 + +1.1 4,145,261 - frwD b3953 0.29 -0.7 4,145,263 - frwD b3953 0.30 +0.1 4,145,264 - frwD b3953 0.30 -0.2 4,145,341 - frwD b3953 0.53 +1.4 4,145,354 + frwD b3953 0.56 -1.7 4,145,354 + frwD b3953 0.56 -0.5 4,145,364 + frwD b3953 0.59 -0.4 4,145,364 + frwD b3953 0.59 -2.1 4,145,372 - frwD b3953 0.62 +0.3 4,145,398 + frwD b3953 0.69 -0.6 4,145,398 + frwD b3953 0.69 -0.1 4,145,411 - frwD b3953 0.73 -0.4 4,145,419 + frwD b3953 0.75 -1.1 4,145,452 + frwD b3953 0.85 +0.2 4,145,499 - +4.0 4,145,500 - +1.8 4,145,525 + +0.4 4,145,612 + yijO b3954 0.14 -0.9 4,145,626 - yijO b3954 0.16 -0.9 4,145,638 + yijO b3954 0.17 -0.9 4,145,658 + yijO b3954 0.20 -0.4 4,145,658 + yijO b3954 0.20 +2.1 4,145,669 + yijO b3954 0.21 +0.8 4,145,669 + yijO b3954 0.21 -0.3 4,145,677 - yijO b3954 0.22 -0.0 4,145,697 - yijO b3954 0.24 +0.8 4,145,697 - yijO b3954 0.24 +0.7 4,145,800 - yijO b3954 0.37 -0.1 4,145,843 + yijO b3954 0.42 -0.3 4,145,853 + yijO b3954 0.43 +0.1 4,145,856 - yijO b3954 0.43 +0.0 4,145,900 + yijO b3954 0.48 +0.2 4,145,900 + yijO b3954 0.48 +1.1 4,146,038 + yijO b3954 0.64 -1.0 4,146,069 + yijO b3954 0.68 +0.7 4,146,091 - yijO b3954 0.71 +2.1 4,146,093 + yijO b3954 0.71 -0.7 4,146,137 + yijO b3954 0.76 -0.8 4,146,140 + yijO b3954 0.76 -0.7 4,146,148 + yijO b3954 0.77 +0.9 4,146,162 + yijO b3954 0.79 -3.1 4,146,162 + yijO b3954 0.79 +0.0 4,146,168 + yijO b3954 0.80 +0.2 4,146,174 + yijO b3954 0.80 -1.1 4,146,179 + yijO b3954 0.81 +1.0 4,146,207 + yijO b3954 0.84 -1.8 4,146,238 - yijO b3954 0.88 +0.5 4,146,242 + yijO b3954 0.88 +1.2 4,146,290 + +1.6 4,146,338 - +0.5 4,146,347 - +0.9 4,146,359 - -0.3 4,146,419 - +0.5 4,146,457 + -0.2 4,146,458 - +0.1 4,146,458 - +0.2
Or see this region's nucleotide sequence