Strain Fitness in Escherichia coli BW25113 around b3949

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
4,139,709	-	ptsA	b3947	0.79	+1.0
4,139,770	-	ptsA	b3947	0.81	+0.1
4,139,782	-	ptsA	b3947	0.81	-0.2
4,139,782	-	ptsA	b3947	0.81	+0.2
4,139,787	-	ptsA	b3947	0.82	-2.0
4,139,878	+	ptsA	b3947	0.85	+1.7
4,139,888	-	ptsA	b3947	0.86	-0.1
4,139,891	+	ptsA	b3947	0.86	-1.3
4,140,001	+				+0.5
4,140,001	+				+1.7
4,140,009	+				+0.1
4,140,096	-				-1.8
4,140,130	+				+0.6
4,140,133	-				-0.6
4,140,133	-				-1.4
4,140,196	+				+1.8
4,140,196	+				-1.6
4,140,204	-				-0.6
4,140,206	+				+0.6
4,140,206	+				-1.1
4,140,285	-				+0.9
4,140,295	+				+2.4
4,140,539	+				+1.4
4,140,667	+	frwC	b3949	0.11	-0.3
4,140,667	+	frwC	b3949	0.11	+1.5
4,140,685	+	frwC	b3949	0.12	+1.7
4,140,685	+	frwC	b3949	0.12	+0.4
4,140,685	+	frwC	b3949	0.12	+1.3
4,140,693	-	frwC	b3949	0.13	+1.7
4,140,756	+	frwC	b3949	0.19	-0.1
4,140,805	+	frwC	b3949	0.23	-0.6
4,140,808	-	frwC	b3949	0.24	+0.9
4,140,827	+	frwC	b3949	0.25	-0.6
4,140,827	+	frwC	b3949	0.25	-1.8
4,140,830	+	frwC	b3949	0.26	-0.6
4,140,895	+	frwC	b3949	0.32	-2.4
4,140,895	+	frwC	b3949	0.32	+1.4
4,140,951	+	frwC	b3949	0.37	+1.1
4,141,002	-	frwC	b3949	0.42	+1.4
4,141,010	+	frwC	b3949	0.42	-1.0
4,141,010	+	frwC	b3949	0.42	+1.6
4,141,051	-	frwC	b3949	0.46	+1.6
4,141,066	+	frwC	b3949	0.47	+0.3
4,141,069	+	frwC	b3949	0.48	-1.8
4,141,069	+	frwC	b3949	0.48	-2.4
4,141,069	+	frwC	b3949	0.48	-1.2
4,141,092	-	frwC	b3949	0.50	+0.7
4,141,105	+	frwC	b3949	0.51	+0.8
4,141,130	-	frwC	b3949	0.53	+0.2
4,141,133	+	frwC	b3949	0.54	-1.9
4,141,154	+	frwC	b3949	0.56	+0.2
4,141,167	+	frwC	b3949	0.57	+0.2
4,141,191	-	frwC	b3949	0.59	-1.9
4,141,194	-	frwC	b3949	0.59	-2.1
4,141,194	-	frwC	b3949	0.59	+0.5
4,141,218	+	frwC	b3949	0.62	+2.0
4,141,218	+	frwC	b3949	0.62	+1.9
4,141,255	+	frwC	b3949	0.65	-0.3
4,141,255	+	frwC	b3949	0.65	+0.4
4,141,311	+	frwC	b3949	0.70	-2.8
4,141,311	+	frwC	b3949	0.70	+0.8
4,141,320	-	frwC	b3949	0.71	+1.1
4,141,320	-	frwC	b3949	0.71	-1.2
4,141,320	-	frwC	b3949	0.71	+0.2
4,141,441	-	frwC	b3949	0.82	-0.1
4,141,448	-	frwC	b3949	0.83	-2.8
4,141,448	-	frwC	b3949	0.83	+0.5
4,141,449	+	frwC	b3949	0.83	-0.3
4,141,450	+	frwC	b3949	0.83	-0.9
4,141,494	+	frwC	b3949	0.87	-0.9
4,141,549	-				+0.4
4,141,549	-				-1.9
4,141,676	+				+0.7
4,141,692	-	frwB	b3950	0.14	+2.2
4,141,692	-	frwB	b3950	0.14	+0.3
4,141,816	+	frwB	b3950	0.53	-0.3
4,141,832	+	frwB	b3950	0.58	-2.7
4,141,832	+	frwB	b3950	0.58	+3.9
4,141,969	+				-1.4
4,142,009	+				-3.1
4,142,028	+				+0.1
4,142,092	+				-1.1
4,142,204	-				-2.2
4,142,223	+				-1.4
4,142,225	+				-0.9
4,142,282	+	pflD	b3951	0.11	+0.1
4,142,282	+	pflD	b3951	0.11	-3.7
4,142,357	-	pflD	b3951	0.15	-0.8
4,142,357	-	pflD	b3951	0.15	+0.7
4,142,370	-	pflD	b3951	0.15	-0.5
4,142,370	-	pflD	b3951	0.15	-1.1
4,142,410	+	pflD	b3951	0.17	+1.9
4,142,461	+	pflD	b3951	0.19	-2.0
4,142,461	+	pflD	b3951	0.19	-0.4
4,142,476	-	pflD	b3951	0.20	+1.6
4,142,476	-	pflD	b3951	0.20	-1.5
4,142,497	-	pflD	b3951	0.21	-2.1
4,142,497	-	pflD	b3951	0.21	-0.5
4,142,499	+	pflD	b3951	0.21	-3.6
4,142,528	-	pflD	b3951	0.22	+0.6
4,142,602	+	pflD	b3951	0.25	+0.1