Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifL and dapF are separated by 36 nucleotides dapF and yigA overlap by 4 nucleotides yigA and xerC overlap by 4 nucleotides xerC and yigB overlap by 1 nucleotides
b4558: yifL - predicted lipoprotein (NCBI), at 3,992,545 to 3,992,748
yifL
b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609
dapF
b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313
yigA
b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206
xerC
b3812: yigB - predicted hydrolase (NCBI), at 3,995,206 to 3,995,922
yigB
Position (kb)
3993
3994
3995 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3992.653 kb on + strand, within yifL at 3992.693 kb on - strand at 3992.693 kb on - strand, within yifL at 3992.728 kb on + strand at 3992.728 kb on + strand at 3992.731 kb on + strand at 3992.738 kb on - strand at 3992.745 kb on + strand at 3992.761 kb on + strand at 3992.783 kb on - strand at 3992.790 kb on - strand at 3992.798 kb on - strand at 3993.601 kb on + strand at 3993.601 kb on + strand at 3993.637 kb on + strand at 3993.647 kb on - strand at 3993.778 kb on - strand, within yigA at 3993.778 kb on - strand, within yigA at 3993.782 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.815 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.829 kb on + strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.854 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.972 kb on - strand, within yigA at 3993.978 kb on - strand, within yigA at 3994.051 kb on - strand, within yigA at 3994.088 kb on - strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.093 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.170 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.202 kb on + strand, within yigA at 3994.210 kb on - strand, within yigA at 3994.279 kb on - strand at 3994.300 kb on - strand at 3994.300 kb on - strand at 3994.314 kb on - strand at 3994.318 kb on + strand at 3994.399 kb on + strand at 3994.765 kb on + strand, within xerC at 3994.776 kb on + strand, within xerC at 3994.932 kb on + strand, within xerC at 3995.087 kb on + strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.095 kb on - strand, within xerC at 3995.113 kb on + strand, within xerC at 3995.165 kb on + strand at 3995.165 kb on + strand at 3995.246 kb on + strand at 3995.246 kb on + strand at 3995.251 kb on + strand at 3995.308 kb on + strand, within yigB at 3995.308 kb on + strand, within yigB
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,992,653 + yifL b4558 0.53 +0.1 3,992,693 - +0.4 3,992,693 - yifL b4558 0.73 -0.3 3,992,728 + +3.4 3,992,728 + -2.3 3,992,731 + +1.1 3,992,738 - +2.2 3,992,745 + +0.7 3,992,761 + +1.0 3,992,783 - +1.2 3,992,790 - -1.3 3,992,798 - +1.4 3,993,601 + +2.9 3,993,601 + -2.6 3,993,637 + +0.5 3,993,647 - -0.8 3,993,778 - yigA b3810 0.24 -1.1 3,993,778 - yigA b3810 0.24 -0.1 3,993,782 - yigA b3810 0.25 +1.7 3,993,813 - yigA b3810 0.29 +2.2 3,993,813 - yigA b3810 0.29 -0.1 3,993,815 + yigA b3810 0.30 -0.3 3,993,827 + yigA b3810 0.31 +0.5 3,993,827 + yigA b3810 0.31 -0.7 3,993,829 + yigA b3810 0.31 -0.7 3,993,837 - yigA b3810 0.33 +1.0 3,993,837 - yigA b3810 0.33 -2.4 3,993,854 - yigA b3810 0.35 +1.3 3,993,892 - yigA b3810 0.40 +1.8 3,993,892 - yigA b3810 0.40 +1.3 3,993,948 - yigA b3810 0.48 +3.1 3,993,948 - yigA b3810 0.48 +2.1 3,993,972 - yigA b3810 0.52 +0.1 3,993,978 - yigA b3810 0.53 +2.3 3,994,051 - yigA b3810 0.63 +3.1 3,994,088 - yigA b3810 0.68 +1.5 3,994,090 + yigA b3810 0.68 +0.9 3,994,090 + yigA b3810 0.68 -0.5 3,994,093 - yigA b3810 0.69 +1.8 3,994,098 - yigA b3810 0.69 +1.5 3,994,098 - yigA b3810 0.69 -1.5 3,994,098 - yigA b3810 0.69 +1.3 3,994,098 - yigA b3810 0.69 -1.3 3,994,098 - yigA b3810 0.69 -0.8 3,994,098 - yigA b3810 0.69 +0.4 3,994,170 + yigA b3810 0.80 +1.3 3,994,192 + yigA b3810 0.83 -1.3 3,994,192 + yigA b3810 0.83 -1.3 3,994,202 + yigA b3810 0.84 +0.8 3,994,210 - yigA b3810 0.85 +2.5 3,994,279 - +2.2 3,994,300 - +0.2 3,994,300 - -0.8 3,994,314 - +0.8 3,994,318 + -1.7 3,994,399 + +1.3 3,994,765 + xerC b3811 0.51 -1.0 3,994,776 + xerC b3811 0.52 -1.0 3,994,932 + xerC b3811 0.69 +2.2 3,995,087 + xerC b3811 0.87 +0.3 3,995,093 - xerC b3811 0.87 -0.0 3,995,093 - xerC b3811 0.87 +0.7 3,995,095 - xerC b3811 0.88 -1.0 3,995,113 + xerC b3811 0.90 +0.2 3,995,165 + -2.2 3,995,165 + -1.2 3,995,246 + +1.6 3,995,246 + -0.2 3,995,251 + +1.9 3,995,308 + yigB b3812 0.14 -1.9 3,995,308 + yigB b3812 0.14 +0.4
Or see this region's nucleotide sequence