Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dgoD and dgoA overlap by 4 nucleotides dgoA and dgoK overlap by 17 nucleotides dgoK and dgoR overlap by 4 nucleotides dgoR and yidX are separated by 277 nucleotides
b4478: dgoD - galactonate dehydratase (NCBI), at 3,869,873 to 3,871,021
dgoD
b4477: dgoA - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase (NCBI), at 3,871,018 to 3,871,635
dgoA
b3693: dgoK - 2-oxo-3-deoxygalactonate kinase (NCBI), at 3,871,619 to 3,872,497
dgoK
b4479: dgoR - predicted DNA-binding transcriptional regulator (NCBI), at 3,872,494 to 3,873,183
dgoR
b3696: yidX - predicted lipoproteinC (NCBI), at 3,873,461 to 3,874,117
yidX
Position (kb)
3871
3872
3873 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3870.755 kb on + strand, within dgoD at 3870.755 kb on + strand, within dgoD at 3870.758 kb on - strand, within dgoD at 3870.826 kb on - strand, within dgoD at 3870.826 kb on - strand, within dgoD at 3870.826 kb on - strand, within dgoD at 3870.939 kb on - strand at 3870.995 kb on - strand at 3871.028 kb on + strand at 3871.028 kb on + strand at 3871.028 kb on + strand at 3871.036 kb on - strand at 3871.040 kb on + strand at 3871.040 kb on + strand at 3871.177 kb on + strand, within dgoA at 3871.321 kb on + strand, within dgoA at 3871.321 kb on + strand, within dgoA at 3871.332 kb on + strand, within dgoA at 3871.332 kb on + strand, within dgoA at 3871.342 kb on - strand, within dgoA at 3871.352 kb on - strand, within dgoA at 3871.352 kb on - strand, within dgoA at 3871.359 kb on - strand, within dgoA at 3871.586 kb on - strand at 3871.592 kb on - strand at 3871.645 kb on + strand at 3871.646 kb on + strand at 3871.664 kb on - strand at 3871.738 kb on + strand, within dgoK at 3871.761 kb on - strand, within dgoK at 3871.762 kb on + strand, within dgoK at 3871.763 kb on - strand, within dgoK at 3871.763 kb on - strand, within dgoK at 3871.774 kb on - strand, within dgoK at 3871.774 kb on - strand, within dgoK at 3871.784 kb on - strand, within dgoK at 3871.784 kb on - strand, within dgoK at 3871.799 kb on - strand, within dgoK at 3871.825 kb on - strand, within dgoK at 3871.835 kb on + strand, within dgoK at 3871.835 kb on + strand, within dgoK at 3871.848 kb on - strand, within dgoK at 3871.848 kb on - strand, within dgoK at 3871.917 kb on - strand, within dgoK at 3871.940 kb on - strand, within dgoK at 3872.090 kb on + strand, within dgoK at 3872.106 kb on - strand, within dgoK at 3872.162 kb on - strand, within dgoK at 3872.162 kb on - strand, within dgoK at 3872.175 kb on - strand, within dgoK at 3872.175 kb on - strand, within dgoK at 3872.219 kb on + strand, within dgoK at 3872.342 kb on - strand, within dgoK at 3872.343 kb on + strand, within dgoK at 3872.353 kb on + strand, within dgoK at 3872.353 kb on + strand, within dgoK at 3872.362 kb on - strand, within dgoK at 3872.422 kb on - strand at 3872.437 kb on + strand at 3872.464 kb on + strand at 3872.496 kb on - strand at 3872.496 kb on - strand at 3872.520 kb on - strand at 3872.520 kb on - strand at 3872.603 kb on - strand, within dgoR at 3872.621 kb on - strand, within dgoR at 3872.639 kb on + strand, within dgoR at 3872.639 kb on + strand, within dgoR at 3872.717 kb on + strand, within dgoR at 3872.727 kb on - strand, within dgoR at 3872.770 kb on + strand, within dgoR at 3872.812 kb on - strand, within dgoR at 3872.841 kb on + strand, within dgoR at 3872.948 kb on + strand, within dgoR at 3872.948 kb on + strand, within dgoR at 3872.968 kb on + strand, within dgoR at 3872.976 kb on + strand, within dgoR at 3872.986 kb on - strand, within dgoR at 3872.986 kb on - strand, within dgoR at 3873.066 kb on - strand, within dgoR at 3873.179 kb on + strand at 3873.190 kb on + strand at 3873.198 kb on - strand at 3873.209 kb on - strand at 3873.209 kb on - strand at 3873.249 kb on + strand at 3873.285 kb on + strand at 3873.285 kb on + strand at 3873.285 kb on - strand at 3873.393 kb on - strand at 3873.400 kb on + strand at 3873.408 kb on - strand at 3873.408 kb on - strand at 3873.433 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,870,755 + dgoD b4478 0.77 +1.1 3,870,755 + dgoD b4478 0.77 +0.9 3,870,758 - dgoD b4478 0.77 -1.2 3,870,826 - dgoD b4478 0.83 -0.5 3,870,826 - dgoD b4478 0.83 -0.7 3,870,826 - dgoD b4478 0.83 +1.4 3,870,939 - +0.3 3,870,995 - -0.3 3,871,028 + -0.7 3,871,028 + +0.6 3,871,028 + -0.7 3,871,036 - -0.0 3,871,040 + +2.2 3,871,040 + -2.8 3,871,177 + dgoA b4477 0.26 -1.4 3,871,321 + dgoA b4477 0.49 +0.4 3,871,321 + dgoA b4477 0.49 -1.9 3,871,332 + dgoA b4477 0.51 +1.4 3,871,332 + dgoA b4477 0.51 -1.8 3,871,342 - dgoA b4477 0.52 -0.7 3,871,352 - dgoA b4477 0.54 -0.1 3,871,352 - dgoA b4477 0.54 -0.9 3,871,359 - dgoA b4477 0.55 +1.7 3,871,586 - -2.0 3,871,592 - +1.0 3,871,645 + -1.5 3,871,646 + +2.2 3,871,664 - -2.8 3,871,738 + dgoK b3693 0.14 -0.1 3,871,761 - dgoK b3693 0.16 +0.5 3,871,762 + dgoK b3693 0.16 -2.2 3,871,763 - dgoK b3693 0.16 -0.6 3,871,763 - dgoK b3693 0.16 -0.1 3,871,774 - dgoK b3693 0.18 -1.1 3,871,774 - dgoK b3693 0.18 -1.3 3,871,784 - dgoK b3693 0.19 +0.6 3,871,784 - dgoK b3693 0.19 -0.8 3,871,799 - dgoK b3693 0.20 -1.5 3,871,825 - dgoK b3693 0.23 +0.1 3,871,835 + dgoK b3693 0.25 +1.2 3,871,835 + dgoK b3693 0.25 -0.4 3,871,848 - dgoK b3693 0.26 -0.5 3,871,848 - dgoK b3693 0.26 -0.3 3,871,917 - dgoK b3693 0.34 +0.5 3,871,940 - dgoK b3693 0.37 +0.2 3,872,090 + dgoK b3693 0.54 -0.3 3,872,106 - dgoK b3693 0.55 -2.2 3,872,162 - dgoK b3693 0.62 +1.1 3,872,162 - dgoK b3693 0.62 -0.5 3,872,175 - dgoK b3693 0.63 +0.1 3,872,175 - dgoK b3693 0.63 -0.9 3,872,219 + dgoK b3693 0.68 +1.5 3,872,342 - dgoK b3693 0.82 -1.0 3,872,343 + dgoK b3693 0.82 -0.7 3,872,353 + dgoK b3693 0.84 +0.3 3,872,353 + dgoK b3693 0.84 -1.0 3,872,362 - dgoK b3693 0.85 +0.3 3,872,422 - -0.3 3,872,437 + +0.6 3,872,464 + -1.4 3,872,496 - -2.0 3,872,496 - -1.3 3,872,520 - -0.3 3,872,520 - -0.8 3,872,603 - dgoR b4479 0.16 -1.0 3,872,621 - dgoR b4479 0.18 +1.6 3,872,639 + dgoR b4479 0.21 -0.9 3,872,639 + dgoR b4479 0.21 -2.4 3,872,717 + dgoR b4479 0.32 +0.2 3,872,727 - dgoR b4479 0.34 -0.5 3,872,770 + dgoR b4479 0.40 -0.8 3,872,812 - dgoR b4479 0.46 +1.2 3,872,841 + dgoR b4479 0.50 -1.3 3,872,948 + dgoR b4479 0.66 -1.6 3,872,948 + dgoR b4479 0.66 +0.6 3,872,968 + dgoR b4479 0.69 -0.5 3,872,976 + dgoR b4479 0.70 -1.2 3,872,986 - dgoR b4479 0.71 +0.3 3,872,986 - dgoR b4479 0.71 +1.0 3,873,066 - dgoR b4479 0.83 -0.7 3,873,179 + +0.9 3,873,190 + -0.9 3,873,198 - +1.7 3,873,209 - +1.0 3,873,209 - -1.9 3,873,249 + +2.0 3,873,285 + +0.5 3,873,285 + +0.8 3,873,285 - +1.6 3,873,393 - -0.6 3,873,400 + -0.5 3,873,408 - -2.0 3,873,408 - -0.0 3,873,433 + +0.2
Or see this region's nucleotide sequence