Strain Fitness in Escherichia coli BW25113 around b3501

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgor and dinQ are separated by 53 nucleotidesdinQ and arsR are separated by 694 nucleotidesarsR and arsB are separated by 53 nucleotides b3500: gor - glutathione reductase (NCBI), at 3,644,322 to 3,645,674 gor b4613: dinQ - Damage inducible, function unknown (RefSeq), at 3,645,728 to 3,645,856 dinQ b3501: arsR - DNA-binding transcriptional repressor (NCBI), at 3,646,551 to 3,646,904 arsR b3502: arsB - arsenical pump membrane protein (VIMSS), at 3,646,958 to 3,648,247 arsB Position (kb) 3646 3647Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3645.690 kb on + strandat 3645.755 kb on + strand, within dinQat 3645.781 kb on + strand, within dinQat 3645.862 kb on + strandat 3645.898 kb on + strandat 3645.898 kb on + strandat 3645.918 kb on - strandat 3645.930 kb on + strandat 3645.956 kb on + strandat 3645.976 kb on + strandat 3646.094 kb on + strandat 3646.100 kb on + strandat 3646.100 kb on + strandat 3646.107 kb on - strandat 3646.117 kb on - strandat 3646.234 kb on + strandat 3646.238 kb on + strandat 3646.249 kb on + strandat 3646.291 kb on + strandat 3646.325 kb on - strandat 3646.325 kb on - strandat 3646.338 kb on - strandat 3646.367 kb on - strandat 3646.560 kb on + strandat 3646.602 kb on + strand, within arsRat 3646.655 kb on + strand, within arsRat 3646.655 kb on + strand, within arsRat 3646.663 kb on - strand, within arsRat 3646.663 kb on - strand, within arsRat 3646.663 kb on - strand, within arsRat 3646.663 kb on - strand, within arsRat 3646.666 kb on - strand, within arsRat 3646.666 kb on - strand, within arsRat 3646.674 kb on - strand, within arsRat 3646.682 kb on + strand, within arsRat 3646.728 kb on + strand, within arsRat 3646.728 kb on + strand, within arsRat 3646.788 kb on - strand, within arsRat 3646.788 kb on - strand, within arsRat 3646.891 kb on - strandat 3647.067 kb on - strandat 3647.073 kb on - strandat 3647.104 kb on + strand, within arsBat 3647.104 kb on + strand, within arsBat 3647.154 kb on + strand, within arsBat 3647.190 kb on + strand, within arsBat 3647.195 kb on + strand, within arsBat 3647.204 kb on + strand, within arsBat 3647.252 kb on + strand, within arsBat 3647.403 kb on + strand, within arsBat 3647.423 kb on + strand, within arsBat 3647.471 kb on - strand, within arsBat 3647.471 kb on - strand, within arsBat 3647.482 kb on + strand, within arsBat 3647.534 kb on + strand, within arsBat 3647.569 kb on + strand, within arsBat 3647.569 kb on + strand, within arsBat 3647.569 kb on + strand, within arsBat 3647.608 kb on + strand, within arsBat 3647.608 kb on + strand, within arsBat 3647.611 kb on - strand, within arsBat 3647.684 kb on - strand, within arsBat 3647.684 kb on - strand, within arsBat 3647.706 kb on + strand, within arsBat 3647.706 kb on - strand, within arsBat 3647.728 kb on + strand, within arsBat 3647.770 kb on + strand, within arsBat 3647.777 kb on + strand, within arsBat 3647.816 kb on + strand, within arsBat 3647.820 kb on + strand, within arsBat 3647.835 kb on + strand, within arsBat 3647.835 kb on + strand, within arsBat 3647.842 kb on - strand, within arsBat 3647.843 kb on - strand, within arsBat 3647.858 kb on - strand, within arsBat 3647.864 kb on - strand, within arsBat 3647.876 kb on + strand, within arsB

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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3,645,690 + -3.2
3,645,755 + dinQ b4613 0.21 -0.6
3,645,781 + dinQ b4613 0.41 +0.7
3,645,862 + +0.8
3,645,898 + -0.7
3,645,898 + -2.7
3,645,918 - -2.9
3,645,930 + -1.6
3,645,956 + -1.2
3,645,976 + -0.1
3,646,094 + +0.2
3,646,100 + +0.0
3,646,100 + +0.7
3,646,107 - +0.5
3,646,117 - -0.5
3,646,234 + -1.9
3,646,238 + -0.5
3,646,249 + +1.3
3,646,291 + -0.6
3,646,325 - -1.9
3,646,325 - -0.9
3,646,338 - -0.8
3,646,367 - -0.2
3,646,560 + +0.7
3,646,602 + arsR b3501 0.14 +1.3
3,646,655 + arsR b3501 0.29 -1.0
3,646,655 + arsR b3501 0.29 +0.5
3,646,663 - arsR b3501 0.32 -1.3
3,646,663 - arsR b3501 0.32 +0.1
3,646,663 - arsR b3501 0.32 -2.4
3,646,663 - arsR b3501 0.32 +0.5
3,646,666 - arsR b3501 0.32 -0.1
3,646,666 - arsR b3501 0.32 +1.2
3,646,674 - arsR b3501 0.35 +0.6
3,646,682 + arsR b3501 0.37 +2.7
3,646,728 + arsR b3501 0.50 -0.2
3,646,728 + arsR b3501 0.50 +1.5
3,646,788 - arsR b3501 0.67 +1.3
3,646,788 - arsR b3501 0.67 +2.9
3,646,891 - -0.5
3,647,067 - +1.1
3,647,073 - +0.2
3,647,104 + arsB b3502 0.11 +0.4
3,647,104 + arsB b3502 0.11 -2.2
3,647,154 + arsB b3502 0.15 +0.2
3,647,190 + arsB b3502 0.18 +0.5
3,647,195 + arsB b3502 0.18 +1.1
3,647,204 + arsB b3502 0.19 +0.5
3,647,252 + arsB b3502 0.23 -0.5
3,647,403 + arsB b3502 0.34 -0.5
3,647,423 + arsB b3502 0.36 +0.2
3,647,471 - arsB b3502 0.40 +1.7
3,647,471 - arsB b3502 0.40 +0.7
3,647,482 + arsB b3502 0.41 +0.4
3,647,534 + arsB b3502 0.45 -1.2
3,647,569 + arsB b3502 0.47 -2.7
3,647,569 + arsB b3502 0.47 -0.3
3,647,569 + arsB b3502 0.47 -0.0
3,647,608 + arsB b3502 0.50 -1.8
3,647,608 + arsB b3502 0.50 +1.3
3,647,611 - arsB b3502 0.51 -3.4
3,647,684 - arsB b3502 0.56 -2.7
3,647,684 - arsB b3502 0.56 +0.5
3,647,706 + arsB b3502 0.58 +1.1
3,647,706 - arsB b3502 0.58 +2.3
3,647,728 + arsB b3502 0.60 -0.4
3,647,770 + arsB b3502 0.63 -2.4
3,647,777 + arsB b3502 0.63 +0.2
3,647,816 + arsB b3502 0.67 -0.5
3,647,820 + arsB b3502 0.67 -0.1
3,647,835 + arsB b3502 0.68 -0.2
3,647,835 + arsB b3502 0.68 -0.7
3,647,842 - arsB b3502 0.69 -1.3
3,647,843 - arsB b3502 0.69 +0.3
3,647,858 - arsB b3502 0.70 -0.2
3,647,864 - arsB b3502 0.70 +0.5
3,647,876 + arsB b3502 0.71 -1.4

Or see this region's nucleotide sequence