Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ugpE and ugpA overlap by 4 nucleotides ugpA and ugpB are separated by 97 nucleotides ugpB and livF are separated by 398 nucleotides
b3451: ugpE - glycerol-3-phosphate transporter subunit (NCBI), at 3,587,205 to 3,588,050
ugpE
b3452: ugpA - glycerol-3-phosphate transporter subunit (NCBI), at 3,588,047 to 3,588,934
ugpA
b3453: ugpB - glycerol-3-phosphate transporter subunit (NCBI), at 3,589,032 to 3,590,348
ugpB
b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460
livF
Position (kb)
3589
3590
3591 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3588.047 kb on - strand at 3588.047 kb on - strand at 3588.067 kb on + strand at 3588.238 kb on + strand, within ugpA at 3588.238 kb on + strand, within ugpA at 3588.254 kb on - strand, within ugpA at 3588.269 kb on + strand, within ugpA at 3588.288 kb on + strand, within ugpA at 3588.288 kb on + strand, within ugpA at 3588.291 kb on - strand, within ugpA at 3588.291 kb on - strand, within ugpA at 3588.399 kb on + strand, within ugpA at 3588.404 kb on + strand, within ugpA at 3588.443 kb on + strand, within ugpA at 3588.443 kb on + strand, within ugpA at 3588.540 kb on + strand, within ugpA at 3588.540 kb on + strand, within ugpA at 3588.548 kb on - strand, within ugpA at 3588.588 kb on + strand, within ugpA at 3588.588 kb on + strand, within ugpA at 3588.608 kb on + strand, within ugpA at 3588.638 kb on + strand, within ugpA at 3588.638 kb on + strand, within ugpA at 3588.647 kb on - strand, within ugpA at 3588.647 kb on - strand, within ugpA at 3588.662 kb on - strand, within ugpA at 3588.662 kb on - strand, within ugpA at 3588.667 kb on - strand, within ugpA at 3588.695 kb on - strand, within ugpA at 3588.996 kb on + strand at 3589.030 kb on + strand at 3589.109 kb on - strand at 3589.109 kb on - strand at 3589.121 kb on - strand at 3589.121 kb on - strand at 3589.134 kb on + strand at 3589.134 kb on + strand at 3589.259 kb on + strand, within ugpB at 3589.259 kb on + strand, within ugpB at 3589.282 kb on + strand, within ugpB at 3589.286 kb on - strand, within ugpB at 3589.292 kb on - strand, within ugpB at 3589.292 kb on - strand, within ugpB at 3589.307 kb on + strand, within ugpB at 3589.307 kb on + strand, within ugpB at 3589.415 kb on + strand, within ugpB at 3589.415 kb on + strand, within ugpB at 3589.417 kb on - strand, within ugpB at 3589.417 kb on - strand, within ugpB at 3589.471 kb on + strand, within ugpB at 3589.471 kb on + strand, within ugpB at 3589.484 kb on + strand, within ugpB at 3589.484 kb on + strand, within ugpB at 3589.487 kb on - strand, within ugpB at 3589.540 kb on - strand, within ugpB at 3589.583 kb on - strand, within ugpB at 3589.583 kb on - strand, within ugpB at 3589.629 kb on - strand, within ugpB at 3589.634 kb on + strand, within ugpB at 3589.674 kb on - strand, within ugpB at 3589.674 kb on - strand, within ugpB at 3589.677 kb on + strand, within ugpB at 3589.677 kb on + strand, within ugpB at 3589.685 kb on - strand, within ugpB at 3589.685 kb on - strand, within ugpB at 3589.777 kb on + strand, within ugpB at 3589.777 kb on + strand, within ugpB at 3589.998 kb on - strand, within ugpB at 3589.998 kb on - strand, within ugpB at 3590.230 kb on + strand at 3590.271 kb on + strand at 3590.305 kb on + strand at 3590.319 kb on + strand at 3590.390 kb on + strand at 3590.403 kb on + strand at 3590.411 kb on - strand at 3590.450 kb on + strand at 3590.459 kb on + strand at 3590.655 kb on + strand at 3590.659 kb on - strand at 3590.659 kb on - strand at 3590.703 kb on - strand at 3590.774 kb on - strand at 3590.810 kb on - strand at 3590.898 kb on - strand, within livF at 3590.898 kb on - strand, within livF at 3590.903 kb on - strand, within livF at 3590.966 kb on - strand, within livF at 3590.966 kb on - strand, within livF at 3590.988 kb on + strand, within livF at 3591.059 kb on - strand, within livF at 3591.061 kb on + strand, within livF at 3591.090 kb on - strand, within livF at 3591.125 kb on - strand, within livF at 3591.164 kb on + strand, within livF at 3591.164 kb on + strand, within livF at 3591.172 kb on - strand, within livF at 3591.197 kb on + strand, within livF at 3591.252 kb on + strand, within livF at 3591.252 kb on + strand, within livF
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,588,047 - +0.2 3,588,047 - -0.6 3,588,067 + +1.3 3,588,238 + ugpA b3452 0.22 -2.2 3,588,238 + ugpA b3452 0.22 -2.7 3,588,254 - ugpA b3452 0.23 +0.7 3,588,269 + ugpA b3452 0.25 +1.7 3,588,288 + ugpA b3452 0.27 -2.3 3,588,288 + ugpA b3452 0.27 -0.3 3,588,291 - ugpA b3452 0.27 +0.0 3,588,291 - ugpA b3452 0.27 -1.0 3,588,399 + ugpA b3452 0.40 -2.2 3,588,404 + ugpA b3452 0.40 -0.3 3,588,443 + ugpA b3452 0.45 +0.8 3,588,443 + ugpA b3452 0.45 +1.0 3,588,540 + ugpA b3452 0.56 +0.9 3,588,540 + ugpA b3452 0.56 +0.7 3,588,548 - ugpA b3452 0.56 +0.8 3,588,588 + ugpA b3452 0.61 -2.7 3,588,588 + ugpA b3452 0.61 -1.2 3,588,608 + ugpA b3452 0.63 +0.4 3,588,638 + ugpA b3452 0.67 -1.6 3,588,638 + ugpA b3452 0.67 -2.7 3,588,647 - ugpA b3452 0.68 -1.7 3,588,647 - ugpA b3452 0.68 -1.5 3,588,662 - ugpA b3452 0.69 +0.2 3,588,662 - ugpA b3452 0.69 +0.6 3,588,667 - ugpA b3452 0.70 -0.7 3,588,695 - ugpA b3452 0.73 +1.3 3,588,996 + -0.7 3,589,030 + +0.8 3,589,109 - +0.1 3,589,109 - +0.2 3,589,121 - +1.6 3,589,121 - +0.2 3,589,134 + +1.1 3,589,134 + -1.6 3,589,259 + ugpB b3453 0.17 +1.0 3,589,259 + ugpB b3453 0.17 +2.0 3,589,282 + ugpB b3453 0.19 -0.1 3,589,286 - ugpB b3453 0.19 -1.1 3,589,292 - ugpB b3453 0.20 -2.7 3,589,292 - ugpB b3453 0.20 -0.4 3,589,307 + ugpB b3453 0.21 -2.2 3,589,307 + ugpB b3453 0.21 -1.6 3,589,415 + ugpB b3453 0.29 -0.4 3,589,415 + ugpB b3453 0.29 +0.6 3,589,417 - ugpB b3453 0.29 -0.9 3,589,417 - ugpB b3453 0.29 -0.6 3,589,471 + ugpB b3453 0.33 -0.3 3,589,471 + ugpB b3453 0.33 +0.1 3,589,484 + ugpB b3453 0.34 +2.2 3,589,484 + ugpB b3453 0.34 -2.6 3,589,487 - ugpB b3453 0.35 +1.1 3,589,540 - ugpB b3453 0.39 -0.7 3,589,583 - ugpB b3453 0.42 +0.3 3,589,583 - ugpB b3453 0.42 -2.0 3,589,629 - ugpB b3453 0.45 +0.3 3,589,634 + ugpB b3453 0.46 -1.7 3,589,674 - ugpB b3453 0.49 -0.8 3,589,674 - ugpB b3453 0.49 +0.5 3,589,677 + ugpB b3453 0.49 -0.5 3,589,677 + ugpB b3453 0.49 -1.2 3,589,685 - ugpB b3453 0.50 +0.2 3,589,685 - ugpB b3453 0.50 -2.6 3,589,777 + ugpB b3453 0.57 -1.4 3,589,777 + ugpB b3453 0.57 +0.3 3,589,998 - ugpB b3453 0.73 +0.0 3,589,998 - ugpB b3453 0.73 -0.4 3,590,230 + +2.5 3,590,271 + -1.9 3,590,305 + -0.9 3,590,319 + +0.6 3,590,390 + +0.4 3,590,403 + -0.0 3,590,411 - -0.9 3,590,450 + +0.7 3,590,459 + -1.0 3,590,655 + -1.7 3,590,659 - -1.4 3,590,659 - -0.0 3,590,703 - -3.0 3,590,774 - +0.6 3,590,810 - +0.3 3,590,898 - livF b3454 0.21 -0.4 3,590,898 - livF b3454 0.21 -0.3 3,590,903 - livF b3454 0.22 -1.3 3,590,966 - livF b3454 0.31 +0.4 3,590,966 - livF b3454 0.31 -1.1 3,590,988 + livF b3454 0.34 -0.3 3,591,059 - livF b3454 0.44 -1.7 3,591,061 + livF b3454 0.44 +0.6 3,591,090 - livF b3454 0.48 -0.8 3,591,125 - livF b3454 0.53 +0.5 3,591,164 + livF b3454 0.58 -0.0 3,591,164 + livF b3454 0.58 -1.0 3,591,172 - livF b3454 0.60 -0.6 3,591,197 + livF b3454 0.63 +0.5 3,591,252 + livF b3454 0.71 +2.2 3,591,252 + livF b3454 0.71 -1.7
Or see this region's nucleotide sequence