Strain Fitness in Escherichia coli BW25113 around b3375

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
3,501,090	+	frlC	b4474	0.88	+0.3
3,501,098	-	frlC	b4474	0.89	-1.2
3,501,100	-	frlC	b4474	0.89	+1.9
3,501,105	+	frlC	b4474	0.89	-0.5
3,501,114	+				-1.7
3,501,114	+				+0.1
3,501,114	+				+0.7
3,501,123	-				+2.5
3,501,123	-				+0.2
3,501,125	+				-0.9
3,501,169	+				-0.6
3,501,169	+				-0.7
3,501,178	+				-0.9
3,501,239	+				-0.7
3,501,240	-				-0.0
3,501,284	+	frlD	b3374	0.12	-0.0
3,501,292	-	frlD	b3374	0.13	-0.4
3,501,330	+	frlD	b3374	0.18	-1.2
3,501,382	-	frlD	b3374	0.25	+0.6
3,501,410	+	frlD	b3374	0.28	-0.2
3,501,498	-	frlD	b3374	0.39	+0.0
3,501,498	-	frlD	b3374	0.39	+1.1
3,501,517	-	frlD	b3374	0.42	+0.0
3,501,522	-	frlD	b3374	0.42	-0.5
3,501,530	+	frlD	b3374	0.43	-0.3
3,501,530	+	frlD	b3374	0.43	-0.4
3,501,530	+	frlD	b3374	0.43	+0.5
3,501,530	+	frlD	b3374	0.43	-0.7
3,501,530	+	frlD	b3374	0.43	-2.8
3,501,536	-	frlD	b3374	0.44	+0.5
3,501,536	-	frlD	b3374	0.44	-0.3
3,501,536	-	frlD	b3374	0.44	+1.2
3,501,538	-	frlD	b3374	0.44	+0.0
3,501,548	-	frlD	b3374	0.46	+0.4
3,501,560	-	frlD	b3374	0.47	-0.4
3,501,801	+	frlD	b3374	0.78	-0.3
3,501,803	+	frlD	b3374	0.78	-0.6
3,501,962	-				-0.6
3,501,964	-				-0.0
3,501,975	+				+0.6
3,501,975	+				-0.9
3,501,980	+				-0.8
3,501,998	-				-0.5
3,501,998	-				-1.9
3,502,109	+				-1.4
3,502,115	-				+1.7
3,502,147	+				+3.3
3,502,157	+	yhfR	b3375	0.11	-0.3
3,502,161	-	yhfR	b3375	0.12	-0.7
3,502,175	-	yhfR	b3375	0.14	-2.0
3,502,205	+	yhfR	b3375	0.18	-1.3
3,502,205	+	yhfR	b3375	0.18	-0.6
3,502,244	-	yhfR	b3375	0.23	-0.7
3,502,244	-	yhfR	b3375	0.23	-0.1
3,502,269	-	yhfR	b3375	0.27	-0.4
3,502,319	-	yhfR	b3375	0.33	-1.4
3,502,326	+	yhfR	b3375	0.34	-1.2
3,502,333	-	yhfR	b3375	0.35	-0.8
3,502,333	-	yhfR	b3375	0.35	+0.2
3,502,337	-	yhfR	b3375	0.36	+0.9
3,502,337	-	yhfR	b3375	0.36	-0.7
3,502,393	+	yhfR	b3375	0.44	-0.7
3,502,401	-	yhfR	b3375	0.45	-1.4
3,502,403	+	yhfR	b3375	0.45	-0.8
3,502,403	+	yhfR	b3375	0.45	-0.1
3,502,403	+	yhfR	b3375	0.45	-1.1
3,502,403	+	yhfR	b3375	0.45	-1.2
3,502,405	-	yhfR	b3375	0.45	+0.4
3,502,411	-	yhfR	b3375	0.46	-1.9
3,502,518	+	yhfR	b3375	0.61	-1.4
3,502,518	-	yhfR	b3375	0.61	-0.9
3,502,518	-	yhfR	b3375	0.61	-2.2
3,502,518	-				-0.1
3,502,529	+	yhfR	b3375	0.62	-2.2
3,502,710	+	yhfR	b3375	0.87	-2.3
3,502,734	+				-1.3
3,502,879	+				+1.3
3,502,884	+				-2.1
3,502,893	-				+0.2
3,502,894	-				+1.0
3,502,894	-				-0.2
3,502,896	+				+1.3
3,502,924	+				+0.2
3,502,924	+				-2.3
3,502,944	+				-1.8
3,502,952	-				+0.7
3,502,957	-				+0.3
3,502,975	-				-1.7
3,502,975	-				+3.3
3,502,987	+				+0.2
3,503,157	+	yhfS	b3376	0.18	-0.0
3,503,157	+	yhfS	b3376	0.18	-0.1
3,503,166	+	yhfS	b3376	0.19	-0.1
3,503,166	+	yhfS	b3376	0.19	-1.1
3,503,217	+	yhfS	b3376	0.24	+0.2
3,503,217	+	yhfS	b3376	0.24	+0.8
3,503,248	-	yhfS	b3376	0.27	+3.0
3,503,257	+	yhfS	b3376	0.28	-0.1
3,503,258	+	yhfS	b3376	0.28	+0.1
3,503,260	-	yhfS	b3376	0.28	+0.9
3,503,260	-	yhfS	b3376	0.28	-2.4
3,503,266	-	yhfS	b3376	0.28	+0.9
3,503,266	-	yhfS	b3376	0.28	-1.2
3,503,278	-	yhfS	b3376	0.30	-2.3
3,503,305	+	yhfS	b3376	0.32	-0.5
3,503,330	-	yhfS	b3376	0.34	-1.5
3,503,330	-	yhfS	b3376	0.34	+2.1
3,503,364	-	yhfS	b3376	0.37	-1.3
3,503,378	+	yhfS	b3376	0.39	-0.1
3,503,386	-	yhfS	b3376	0.40	-1.9
3,503,386	-	yhfS	b3376	0.40	-1.5
3,503,409	+	yhfS	b3376	0.42	+0.7
3,503,409	+	yhfS	b3376	0.42	+0.2
3,503,427	+	yhfS	b3376	0.43	-0.9
3,503,427	+	yhfS	b3376	0.43	+2.9
3,503,510	+	yhfS	b3376	0.51	+0.7
3,503,520	+	yhfS	b3376	0.52	-0.4
3,503,527	+	yhfS	b3376	0.52	+1.7
3,503,588	+	yhfS	b3376	0.58	+0.6
3,503,604	-	yhfS	b3376	0.60	-0.3
3,503,718	+	yhfS	b3376	0.70	+1.6
3,503,737	+	yhfS	b3376	0.72	+1.1
3,503,760	+	yhfS	b3376	0.74	-0.9