Strain Fitness in Escherichia coli BW25113 around b3335
Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | 3-methyl-1-butanol 1.25 vol% |
---|---|---|---|---|---|
remove | |||||
3,462,593 | + | yheK | b3333 | 0.56 | +0.2 |
3,462,593 | + | yheK | b3333 | 0.56 | -0.7 |
3,462,621 | - | yheK | b3333 | 0.58 | +1.0 |
3,462,690 | - | yheK | b3333 | 0.64 | +0.5 |
3,462,690 | - | yheK | b3333 | 0.64 | -1.7 |
3,462,763 | - | yheK | b3333 | 0.70 | +0.5 |
3,462,763 | - | yheK | b3333 | 0.70 | -2.1 |
3,462,837 | + | yheK | b3333 | 0.77 | -2.1 |
3,462,837 | + | yheK | b3333 | 0.77 | +0.0 |
3,462,864 | + | yheK | b3333 | 0.79 | +0.7 |
3,462,870 | + | yheK | b3333 | 0.80 | +0.0 |
3,462,870 | + | yheK | b3333 | 0.80 | +1.0 |
3,462,878 | - | yheK | b3333 | 0.80 | -0.6 |
3,462,891 | - | yheK | b3333 | 0.81 | -0.5 |
3,462,905 | + | yheK | b3333 | 0.83 | -2.7 |
3,462,907 | + | yheK | b3333 | 0.83 | +0.5 |
3,462,915 | + | yheK | b3333 | 0.83 | -1.7 |
3,462,928 | + | yheK | b3333 | 0.85 | +0.4 |
3,462,928 | - | yheK | b3333 | 0.85 | +2.0 |
3,462,928 | - | yheK | b3333 | 0.85 | +0.3 |
3,462,945 | + | yheK | b3333 | 0.86 | +2.5 |
3,462,948 | - | yheK | b3333 | 0.86 | -1.3 |
3,462,956 | - | yheK | b3333 | 0.87 | -0.6 |
3,462,996 | - | -0.4 | |||
3,462,996 | - | +1.1 | |||
3,462,999 | + | -1.7 | |||
3,463,002 | - | -1.7 | |||
3,463,007 | - | -1.5 | |||
3,463,022 | + | -0.9 | |||
3,463,022 | + | +0.9 | |||
3,463,030 | - | -2.9 | |||
3,463,032 | + | -2.0 | |||
3,463,032 | + | -2.6 | |||
3,463,040 | - | +0.5 | |||
3,463,040 | - | +2.0 | |||
3,463,040 | - | +1.0 | |||
3,463,040 | - | -0.1 | |||
3,463,040 | - | -1.9 | |||
3,463,076 | + | +1.4 | |||
3,463,077 | + | -1.1 | |||
3,463,081 | - | -2.9 | |||
3,463,084 | - | -1.1 | |||
3,463,085 | - | +1.6 | |||
3,463,085 | - | +0.2 | |||
3,463,085 | - | -1.1 | |||
3,463,085 | - | -1.6 | |||
3,463,095 | - | -1.7 | |||
3,463,115 | - | -0.9 | |||
3,463,161 | - | pshM | b3334 | 0.12 | +0.8 |
3,463,161 | - | pshM | b3334 | 0.12 | +2.1 |
3,463,215 | + | pshM | b3334 | 0.24 | -0.7 |
3,463,217 | + | pshM | b3334 | 0.24 | +0.7 |
3,463,217 | + | pshM | b3334 | 0.24 | +0.6 |
3,463,217 | + | pshM | b3334 | 0.24 | +1.8 |
3,463,223 | - | pshM | b3334 | 0.26 | -1.8 |
3,463,233 | + | pshM | b3334 | 0.28 | -0.7 |
3,463,252 | + | pshM | b3334 | 0.32 | -1.3 |
3,463,270 | + | pshM | b3334 | 0.36 | +0.6 |
3,463,294 | + | pshM | b3334 | 0.41 | +2.4 |
3,463,302 | - | pshM | b3334 | 0.43 | -2.2 |
3,463,302 | - | pshM | b3334 | 0.43 | +0.3 |
3,463,393 | - | pshM | b3334 | 0.63 | -0.4 |
3,463,420 | - | pshM | b3334 | 0.68 | +2.5 |
3,463,420 | - | pshM | b3334 | 0.68 | -0.8 |
3,463,459 | + | pshM | b3334 | 0.77 | +1.3 |
3,463,459 | + | pshM | b3334 | 0.77 | +2.2 |
3,463,470 | + | pshM | b3334 | 0.79 | -0.8 |
3,463,470 | + | pshM | b3334 | 0.79 | +0.2 |
3,463,486 | - | pshM | b3334 | 0.83 | +0.7 |
3,463,486 | - | pshM | b3334 | 0.83 | +0.4 |
3,463,488 | - | pshM | b3334 | 0.83 | +0.2 |
3,463,519 | - | -1.9 | |||
3,463,533 | - | +0.9 | |||
3,463,535 | - | +0.1 | |||
3,463,544 | + | +1.8 | |||
3,463,546 | + | -2.3 | |||
3,463,552 | - | -0.7 | |||
3,463,624 | + | -2.4 | |||
3,463,624 | + | +0.7 | |||
3,463,639 | - | gspO | b3335 | 0.11 | -1.2 |
3,463,639 | - | gspO | b3335 | 0.11 | +1.7 |
3,463,640 | - | gspO | b3335 | 0.11 | -3.0 |
3,463,640 | - | gspO | b3335 | 0.11 | +0.8 |
3,463,647 | - | gspO | b3335 | 0.12 | -0.5 |
3,463,654 | + | gspO | b3335 | 0.13 | +1.1 |
3,463,654 | + | gspO | b3335 | 0.13 | +3.4 |
3,463,696 | - | gspO | b3335 | 0.19 | +1.7 |
3,463,716 | + | gspO | b3335 | 0.22 | +2.1 |
3,463,716 | + | gspO | b3335 | 0.22 | +0.8 |
3,463,717 | + | gspO | b3335 | 0.22 | +1.3 |
3,463,719 | + | gspO | b3335 | 0.23 | +0.6 |
3,463,719 | + | gspO | b3335 | 0.23 | -1.3 |
3,463,720 | + | gspO | b3335 | 0.23 | -0.9 |
3,463,720 | + | gspO | b3335 | 0.23 | -0.1 |
3,463,725 | - | gspO | b3335 | 0.24 | -0.6 |
3,463,728 | - | gspO | b3335 | 0.24 | +1.7 |
3,463,759 | + | gspO | b3335 | 0.29 | +0.6 |
3,463,767 | + | gspO | b3335 | 0.30 | -0.1 |
3,463,768 | - | gspO | b3335 | 0.30 | -0.0 |
3,463,775 | + | gspO | b3335 | 0.31 | +0.1 |
3,463,775 | + | gspO | b3335 | 0.31 | +0.0 |
3,463,775 | - | gspO | b3335 | 0.31 | +0.8 |
3,463,775 | - | gspO | b3335 | 0.31 | +0.6 |
3,463,777 | + | gspO | b3335 | 0.31 | +0.0 |
3,463,812 | - | gspO | b3335 | 0.36 | -0.7 |
3,463,832 | - | gspO | b3335 | 0.39 | -0.5 |
3,463,844 | - | gspO | b3335 | 0.41 | +1.0 |
3,463,858 | + | gspO | b3335 | 0.43 | -0.6 |
3,463,878 | + | gspO | b3335 | 0.46 | -1.6 |
3,463,893 | + | gspO | b3335 | 0.48 | +1.6 |
3,463,918 | + | gspO | b3335 | 0.52 | +0.5 |
3,463,918 | + | gspO | b3335 | 0.52 | -0.3 |
3,463,922 | + | gspO | b3335 | 0.53 | -0.3 |
3,463,963 | + | gspO | b3335 | 0.59 | +2.7 |
3,463,963 | + | gspO | b3335 | 0.59 | -2.0 |
3,463,965 | + | gspO | b3335 | 0.59 | -1.0 |
3,463,971 | - | gspO | b3335 | 0.60 | -2.6 |
3,463,974 | - | gspO | b3335 | 0.60 | +4.5 |
3,463,991 | - | gspO | b3335 | 0.63 | -1.0 |
3,463,995 | - | gspO | b3335 | 0.63 | +0.9 |
3,464,030 | - | gspO | b3335 | 0.69 | +0.8 |
3,464,062 | + | gspO | b3335 | 0.73 | -2.2 |
3,464,062 | + | gspO | b3335 | 0.73 | +0.1 |
3,464,063 | - | gspO | b3335 | 0.73 | +0.7 |
3,464,072 | - | gspO | b3335 | 0.75 | +2.2 |
3,464,100 | + | gspO | b3335 | 0.79 | -0.3 |
3,464,121 | - | gspO | b3335 | 0.82 | -0.8 |
3,464,121 | - | gspO | b3335 | 0.82 | -0.7 |
3,464,125 | - | gspO | b3335 | 0.83 | +0.4 |
3,464,126 | - | gspO | b3335 | 0.83 | +2.0 |
3,464,158 | + | gspO | b3335 | 0.87 | -0.2 |
3,464,192 | + | -1.1 | |||
3,464,210 | + | +1.2 | |||
3,464,210 | - | +0.1 | |||
3,464,215 | + | +0.1 | |||
3,464,215 | + | -2.0 | |||
3,464,218 | - | -0.2 | |||
3,464,249 | + | -1.2 | |||
3,464,257 | + | -1.1 | |||
3,464,266 | - | -2.2 | |||
3,464,266 | - | +1.0 | |||
3,464,294 | - | +0.3 | |||
3,464,296 | - | +0.2 | |||
3,464,330 | + | bfr | b3336 | 0.12 | -1.2 |
3,464,338 | - | bfr | b3336 | 0.14 | +0.4 |
3,464,360 | - | bfr | b3336 | 0.19 | +0.3 |
3,464,360 | - | bfr | b3336 | 0.19 | -0.1 |
3,464,406 | - | bfr | b3336 | 0.28 | +0.4 |
3,464,423 | + | bfr | b3336 | 0.32 | -0.3 |
3,464,427 | - | bfr | b3336 | 0.33 | -0.4 |
3,464,427 | - | bfr | b3336 | 0.33 | -0.6 |
3,464,427 | - | bfr | b3336 | 0.33 | -0.4 |
3,464,438 | - | bfr | b3336 | 0.35 | -0.9 |
3,464,473 | - | bfr | b3336 | 0.42 | -0.4 |
3,464,485 | - | bfr | b3336 | 0.45 | +1.0 |
3,464,498 | - | bfr | b3336 | 0.48 | +1.0 |
3,464,502 | - | bfr | b3336 | 0.48 | -0.3 |
3,464,503 | - | bfr | b3336 | 0.49 | +0.2 |
3,464,504 | + | bfr | b3336 | 0.49 | +0.3 |
3,464,504 | + | bfr | b3336 | 0.49 | +0.7 |
3,464,517 | + | bfr | b3336 | 0.52 | -0.1 |
3,464,523 | + | bfr | b3336 | 0.53 | +2.1 |
3,464,523 | + | bfr | b3336 | 0.53 | -0.1 |
3,464,523 | + | bfr | b3336 | 0.53 | -0.9 |
3,464,572 | + | bfr | b3336 | 0.63 | -1.1 |
3,464,580 | - | bfr | b3336 | 0.65 | -0.5 |
3,464,587 | + | bfr | b3336 | 0.66 | -0.5 |
3,464,587 | - | bfr | b3336 | 0.66 | -2.8 |
3,464,587 | - | bfr | b3336 | 0.66 | +1.6 |
3,464,606 | + | bfr | b3336 | 0.70 | +0.7 |
3,464,614 | - | bfr | b3336 | 0.72 | +1.7 |
3,464,614 | - | bfr | b3336 | 0.72 | +0.5 |
3,464,618 | - | bfr | b3336 | 0.73 | -0.0 |
3,464,618 | - | bfr | b3336 | 0.73 | -3.2 |
3,464,618 | - | bfr | b3336 | 0.73 | +0.1 |
3,464,618 | - | bfr | b3336 | 0.73 | -1.1 |
3,464,618 | - | bfr | b3336 | 0.73 | -0.7 |
3,464,619 | - | bfr | b3336 | 0.73 | +1.2 |
3,464,623 | + | bfr | b3336 | 0.74 | -0.9 |
3,464,623 | + | bfr | b3336 | 0.74 | +2.0 |
3,464,623 | - | bfr | b3336 | 0.74 | +0.4 |
3,464,623 | - | bfr | b3336 | 0.74 | -1.9 |
3,464,626 | - | bfr | b3336 | 0.74 | -0.2 |
3,464,631 | - | bfr | b3336 | 0.75 | -0.2 |
3,464,641 | - | bfr | b3336 | 0.78 | -0.1 |
3,464,641 | - | bfr | b3336 | 0.78 | -2.0 |
3,464,681 | - | bfr | b3336 | 0.86 | +0.5 |
3,464,684 | - | bfr | b3336 | 0.87 | +0.4 |
3,464,706 | - | -0.9 | |||
3,464,707 | + | -1.2 | |||
3,464,707 | + | -2.7 | |||
3,464,708 | - | +1.0 | |||
3,464,729 | - | +0.6 | |||
3,464,729 | - | +0.9 | |||
3,464,769 | - | -0.7 | |||
3,464,778 | + | -2.8 | |||
3,464,778 | + | -1.4 | |||
3,464,778 | + | +0.2 | |||
3,464,786 | - | -0.9 | |||
3,464,786 | - | +0.3 | |||
3,464,786 | - | +0.0 | |||
3,464,788 | + | -1.5 | |||
3,464,788 | + | +1.2 | |||
3,464,788 | + | -0.0 | |||
3,464,788 | + | -0.8 | |||
3,464,788 | + | +2.1 | |||
3,464,788 | + | +0.1 | |||
3,464,796 | - | -0.3 | |||
3,464,796 | - | -0.5 | |||
3,464,796 | - | -0.0 | |||
3,464,809 | - | -0.3 | |||
3,464,809 | - | +0.9 | |||
3,464,815 | + | +0.1 | |||
3,464,819 | - | -0.1 | |||
3,464,853 | + | bfd | b3337 | 0.17 | +0.9 |
3,464,862 | - | bfd | b3337 | 0.22 | -1.8 |
3,464,874 | + | bfd | b3337 | 0.28 | -0.7 |
3,464,880 | + | bfd | b3337 | 0.31 | -0.9 |
3,464,880 | + | bfd | b3337 | 0.31 | -0.9 |
3,464,880 | + | bfd | b3337 | 0.31 | +1.5 |
3,464,880 | + | bfd | b3337 | 0.31 | -2.9 |
3,464,887 | + | bfd | b3337 | 0.35 | +1.3 |
3,464,888 | - | bfd | b3337 | 0.35 | -0.9 |
3,464,889 | + | bfd | b3337 | 0.36 | -2.8 |
3,464,892 | - | bfd | b3337 | 0.37 | -0.3 |
3,464,957 | + | bfd | b3337 | 0.71 | -1.1 |
3,464,958 | + | bfd | b3337 | 0.71 | +0.5 |
3,464,989 | + | bfd | b3337 | 0.87 | -0.8 |
3,464,999 | - | +0.3 | |||
3,465,000 | - | -0.3 | |||
3,465,009 | + | +0.2 | |||
3,465,011 | + | -0.7 | |||
3,465,011 | + | +2.3 | |||
3,465,011 | + | +0.5 | |||
3,465,011 | + | +1.0 | |||
3,465,017 | - | +0.4 | |||
3,465,017 | - | -0.1 | |||
3,465,017 | - | +2.5 | |||
3,465,017 | - | +0.2 | |||
3,465,017 | - | +1.0 | |||
3,465,017 | - | -0.7 | |||
3,465,021 | + | +3.2 | |||
3,465,023 | + | +0.8 | |||
3,465,023 | + | +1.8 | |||
3,465,132 | - | +0.5 | |||
3,465,136 | - | +1.5 | |||
3,465,160 | + | +1.3 | |||
3,465,160 | + | +0.2 | |||
3,465,167 | + | +0.4 | |||
3,465,168 | - | -0.8 |
Or see this region's nucleotide sequence