Strain Fitness in Escherichia coli BW25113 around b3228

Experiment: 3-methyl-1-butanol 1.25 vol%

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	3-methyl-1-butanol 1.25 vol%
3,373,301	+	dcuD	b3227	0.30	-0.5
3,373,340	-	dcuD	b3227	0.33	+1.5
3,373,340	-	dcuD	b3227	0.33	+1.9
3,373,381	+	dcuD	b3227	0.36	-0.5
3,373,391	+	dcuD	b3227	0.37	-0.2
3,373,406	-	dcuD	b3227	0.38	+0.2
3,373,538	-	dcuD	b3227	0.47	-0.7
3,373,721	+	dcuD	b3227	0.61	+1.9
3,373,745	-	dcuD	b3227	0.62	+0.8
3,373,773	-	dcuD	b3227	0.64	+0.4
3,373,773	-	dcuD	b3227	0.64	+1.1
3,373,896	+	dcuD	b3227	0.73	+0.3
3,373,897	-	dcuD	b3227	0.74	-0.2
3,373,924	+	dcuD	b3227	0.76	+1.4
3,373,924	+	dcuD	b3227	0.76	-0.5
3,373,932	-	dcuD	b3227	0.76	-0.1
3,373,932	-	dcuD	b3227	0.76	-0.9
3,373,933	-				+1.3
3,373,933	-	dcuD	b3227	0.76	+0.3
3,373,984	+	dcuD	b3227	0.80	+0.8
3,374,004	-	dcuD	b3227	0.81	+0.7
3,374,052	+	dcuD	b3227	0.85	+0.0
3,374,060	-	dcuD	b3227	0.85	+0.1
3,374,103	+	dcuD	b3227	0.89	+0.9
3,374,128	+				+0.3
3,374,209	-				+0.9
3,374,249	+				+0.5
3,374,249	+				-1.6
3,374,306	-				+1.3
3,374,306	-				+1.8
3,374,366	-	sspB	b3228	0.13	+1.9
3,374,472	-	sspB	b3228	0.34	+1.1
3,374,472	-	sspB	b3228	0.34	-0.1
3,374,484	-	sspB	b3228	0.37	-1.3
3,374,484	-	sspB	b3228	0.37	-0.1
3,374,514	+	sspB	b3228	0.43	-0.7
3,374,543	-	sspB	b3228	0.49	-0.8
3,374,543	-	sspB	b3228	0.49	-0.7
3,374,563	+	sspB	b3228	0.53	+1.7
3,374,667	+	sspB	b3228	0.73	+2.1
3,374,667	+	sspB	b3228	0.73	+4.4
3,374,675	-	sspB	b3228	0.75	-0.7
3,374,675	-	sspB	b3228	0.75	+0.3
3,374,684	+	sspB	b3228	0.77	+2.5
3,374,684	+	sspB	b3228	0.77	+2.6
3,374,966	-	sspA	b3229	0.25	+2.5
3,374,976	+	sspA	b3229	0.27	+3.8
3,374,976	+	sspA	b3229	0.27	+4.4
3,375,071	-	sspA	b3229	0.42	-1.4
3,375,073	-	sspA	b3229	0.42	+1.4
3,375,084	-	sspA	b3229	0.44	+5.2
3,375,098	+	sspA	b3229	0.46	+2.0
3,375,098	+	sspA	b3229	0.46	+5.1
3,375,113	-	sspA	b3229	0.48	-0.3
3,375,189	-	sspA	b3229	0.60	+1.7
3,375,220	-	sspA	b3229	0.65	-1.3
3,375,220	-	sspA	b3229	0.65	+1.5
3,375,220	-	sspA	b3229	0.65	+1.7
3,375,236	-	sspA	b3229	0.68	-1.3
3,375,353	+	sspA	b3229	0.86	+4.1
3,375,353	+	sspA	b3229	0.86	+4.5
3,375,515	-				+2.7