Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhcG and yhcH are separated by 59 nucleotides yhcH and yhcI overlap by 4 nucleotides yhcI and nanE overlap by 4 nucleotides nanE and nanT are separated by 47 nucleotides
b3220: yhcG - hypothetical protein (NCBI), at 3,365,849 to 3,366,976
yhcG
b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500
yhcH
b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372
yhcI
b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058
nanE
b3224: nanT - sialic acid transporter (VIMSS), at 3,369,106 to 3,370,596
nanT
Position (kb)
3367
3368
3369 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3366.683 kb on - strand, within yhcG at 3366.683 kb on - strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.684 kb on + strand, within yhcG at 3366.692 kb on - strand, within yhcG at 3366.720 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.760 kb on + strand, within yhcG at 3366.808 kb on + strand, within yhcG at 3366.856 kb on - strand, within yhcG at 3366.857 kb on + strand, within yhcG at 3366.906 kb on + strand at 3366.924 kb on - strand at 3366.955 kb on - strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.960 kb on + strand at 3366.967 kb on - strand at 3366.968 kb on - strand at 3367.219 kb on - strand, within yhcH at 3367.221 kb on + strand, within yhcH at 3367.238 kb on + strand, within yhcH at 3367.246 kb on - strand, within yhcH at 3367.260 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.273 kb on - strand, within yhcH at 3367.348 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.386 kb on - strand, within yhcH at 3367.444 kb on - strand, within yhcH at 3367.452 kb on - strand, within yhcH at 3367.454 kb on + strand at 3367.480 kb on - strand at 3367.480 kb on - strand at 3367.489 kb on + strand at 3367.523 kb on - strand at 3367.600 kb on + strand, within yhcI at 3367.600 kb on + strand, within yhcI at 3367.666 kb on - strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.678 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.691 kb on + strand, within yhcI at 3367.725 kb on + strand, within yhcI at 3367.728 kb on + strand, within yhcI at 3367.768 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.855 kb on - strand, within yhcI at 3367.901 kb on + strand, within yhcI at 3368.002 kb on + strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.179 kb on - strand, within yhcI at 3368.184 kb on - strand, within yhcI at 3368.285 kb on + strand at 3368.327 kb on - strand at 3368.368 kb on + strand at 3368.585 kb on + strand, within nanE at 3368.593 kb on - strand, within nanE at 3368.696 kb on - strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.699 kb on + strand, within nanE at 3368.703 kb on + strand, within nanE at 3368.768 kb on + strand, within nanE at 3368.780 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.791 kb on + strand, within nanE at 3368.887 kb on + strand, within nanE at 3368.989 kb on - strand, within nanE at 3368.989 kb on - strand, within nanE at 3369.084 kb on + strand at 3369.297 kb on + strand, within nanT
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,366,683 - yhcG b3220 0.74 -0.1 3,366,683 - yhcG b3220 0.74 -0.5 3,366,684 + yhcG b3220 0.74 +1.2 3,366,684 + yhcG b3220 0.74 -0.9 3,366,692 - yhcG b3220 0.75 +0.8 3,366,720 + yhcG b3220 0.77 +0.6 3,366,760 + yhcG b3220 0.81 -0.9 3,366,760 + yhcG b3220 0.81 -2.8 3,366,808 + yhcG b3220 0.85 -1.6 3,366,856 - yhcG b3220 0.89 -0.8 3,366,857 + yhcG b3220 0.89 +1.1 3,366,906 + +0.4 3,366,924 - -0.0 3,366,955 - -2.0 3,366,960 + -0.1 3,366,960 + +1.2 3,366,960 + +0.3 3,366,967 - -0.7 3,366,968 - +2.0 3,367,219 - yhcH b3221 0.39 -1.0 3,367,221 + yhcH b3221 0.40 +0.6 3,367,238 + yhcH b3221 0.43 -0.6 3,367,246 - yhcH b3221 0.45 +0.0 3,367,260 - yhcH b3221 0.48 +1.0 3,367,273 - yhcH b3221 0.51 +2.5 3,367,273 - yhcH b3221 0.51 +0.8 3,367,348 - yhcH b3221 0.67 -2.4 3,367,386 - yhcH b3221 0.75 +0.8 3,367,386 - yhcH b3221 0.75 +1.6 3,367,444 - yhcH b3221 0.88 -0.9 3,367,452 - yhcH b3221 0.89 -0.7 3,367,454 + +0.3 3,367,480 - +1.2 3,367,480 - +1.3 3,367,489 + -1.1 3,367,523 - +3.8 3,367,600 + yhcI b3222 0.12 -0.4 3,367,600 + yhcI b3222 0.12 -2.4 3,367,666 - yhcI b3222 0.19 +0.8 3,367,678 + yhcI b3222 0.21 +0.2 3,367,678 + yhcI b3222 0.21 -3.4 3,367,691 + yhcI b3222 0.22 -0.7 3,367,691 + yhcI b3222 0.22 -1.1 3,367,725 + yhcI b3222 0.26 +1.3 3,367,728 + yhcI b3222 0.26 -1.1 3,367,768 - yhcI b3222 0.31 -0.2 3,367,855 - yhcI b3222 0.41 +0.2 3,367,855 - yhcI b3222 0.41 -3.4 3,367,901 + yhcI b3222 0.46 -0.1 3,368,002 + yhcI b3222 0.58 +0.5 3,368,179 - yhcI b3222 0.78 +0.6 3,368,179 - yhcI b3222 0.78 -0.8 3,368,184 - yhcI b3222 0.78 -1.2 3,368,285 + +0.9 3,368,327 - -0.2 3,368,368 + +0.7 3,368,585 + nanE b3223 0.31 +0.8 3,368,593 - nanE b3223 0.32 -0.1 3,368,696 - nanE b3223 0.47 +0.5 3,368,699 + nanE b3223 0.48 -1.2 3,368,699 + nanE b3223 0.48 +1.0 3,368,703 + nanE b3223 0.48 -0.6 3,368,768 + nanE b3223 0.58 +0.7 3,368,780 + nanE b3223 0.60 +0.7 3,368,791 + nanE b3223 0.61 -0.1 3,368,791 + nanE b3223 0.61 -0.2 3,368,887 + nanE b3223 0.75 -0.1 3,368,989 - nanE b3223 0.90 +1.2 3,368,989 - nanE b3223 0.90 +1.2 3,369,084 + -0.0 3,369,297 + nanT b3224 0.13 -1.2
Or see this region's nucleotide sequence