Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yraQ and yraR are separated by 72 nucleotides yraR and yhbO are separated by 127 nucleotides yhbO and yhbP overlap by 21 nucleotides
b3151: yraQ - predicted permease (NCBI), at 3,295,120 to 3,296,160
yraQ
b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868
yraR
b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514
yhbO
b3154: yhbP - hypothetical protein (NCBI), at 3,297,494 to 3,297,937
yhbP
Position (kb)
3296
3297 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3295.249 kb on - strand, within yraQ at 3295.288 kb on + strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.384 kb on + strand, within yraQ at 3295.392 kb on - strand, within yraQ at 3295.394 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.451 kb on - strand, within yraQ at 3295.461 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.618 kb on - strand, within yraQ at 3295.633 kb on - strand, within yraQ at 3295.690 kb on + strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.824 kb on - strand, within yraQ at 3295.850 kb on + strand, within yraQ at 3295.911 kb on - strand, within yraQ at 3295.915 kb on - strand, within yraQ at 3296.007 kb on + strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.059 kb on - strand at 3296.059 kb on - strand at 3296.196 kb on + strand at 3296.201 kb on + strand at 3296.201 kb on + strand at 3296.221 kb on - strand at 3296.221 kb on - strand at 3296.289 kb on - strand at 3296.289 kb on - strand at 3296.294 kb on - strand at 3296.441 kb on - strand, within yraR at 3296.441 kb on - strand, within yraR at 3296.466 kb on - strand, within yraR at 3296.549 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.687 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.819 kb on + strand at 3296.819 kb on + strand at 3296.830 kb on + strand at 3296.927 kb on - strand at 3296.992 kb on + strand at 3296.992 kb on + strand at 3297.032 kb on - strand at 3297.149 kb on - strand, within yhbO at 3297.149 kb on - strand, within yhbO at 3297.295 kb on - strand, within yhbO at 3297.383 kb on - strand, within yhbO at 3297.383 kb on - strand, within yhbO at 3297.383 kb on - strand, within yhbO at 3297.395 kb on - strand, within yhbO at 3297.395 kb on - strand, within yhbO at 3297.432 kb on + strand, within yhbO at 3297.491 kb on + strand at 3297.491 kb on + strand at 3297.515 kb on - strand at 3297.553 kb on - strand, within yhbP at 3297.632 kb on + strand, within yhbP at 3297.632 kb on + strand, within yhbP at 3297.639 kb on + strand, within yhbP at 3297.667 kb on + strand, within yhbP at 3297.740 kb on - strand, within yhbP at 3297.740 kb on - strand, within yhbP at 3297.770 kb on + strand, within yhbP at 3297.842 kb on + strand, within yhbP at 3297.852 kb on + strand, within yhbP at 3297.857 kb on + strand, within yhbP
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,295,249 - yraQ b3151 0.12 +0.7 3,295,288 + yraQ b3151 0.16 +0.6 3,295,350 - yraQ b3151 0.22 +0.5 3,295,350 - yraQ b3151 0.22 -1.1 3,295,384 + yraQ b3151 0.25 -0.5 3,295,392 - yraQ b3151 0.26 +1.0 3,295,394 + yraQ b3151 0.26 -0.1 3,295,443 + yraQ b3151 0.31 -1.0 3,295,443 + yraQ b3151 0.31 +0.8 3,295,451 - yraQ b3151 0.32 -0.6 3,295,461 - yraQ b3151 0.33 +1.0 3,295,559 - yraQ b3151 0.42 +0.8 3,295,559 - yraQ b3151 0.42 -0.2 3,295,618 - yraQ b3151 0.48 -0.5 3,295,633 - yraQ b3151 0.49 +0.4 3,295,690 + yraQ b3151 0.55 -1.2 3,295,791 - yraQ b3151 0.64 -0.8 3,295,791 - yraQ b3151 0.64 -1.2 3,295,824 - yraQ b3151 0.68 +0.4 3,295,850 + yraQ b3151 0.70 -1.0 3,295,911 - yraQ b3151 0.76 -2.2 3,295,915 - yraQ b3151 0.76 +0.3 3,296,007 + yraQ b3151 0.85 +2.1 3,296,053 - yraQ b3151 0.90 -0.6 3,296,053 - yraQ b3151 0.90 +0.5 3,296,059 - -0.2 3,296,059 - +1.7 3,296,196 + +3.0 3,296,201 + -0.7 3,296,201 + -0.1 3,296,221 - +1.1 3,296,221 - -0.1 3,296,289 - -1.1 3,296,289 - +1.1 3,296,294 - -0.4 3,296,441 - yraR b3152 0.33 -0.0 3,296,441 - yraR b3152 0.33 -1.6 3,296,466 - yraR b3152 0.37 -0.5 3,296,549 + yraR b3152 0.50 -0.2 3,296,661 + yraR b3152 0.67 -0.7 3,296,661 + yraR b3152 0.67 +0.4 3,296,687 - yraR b3152 0.71 +0.1 3,296,737 - yraR b3152 0.79 -1.3 3,296,737 - yraR b3152 0.79 -0.6 3,296,819 + -0.5 3,296,819 + -0.6 3,296,830 + -2.7 3,296,927 - -0.2 3,296,992 + +0.6 3,296,992 + +0.8 3,297,032 - -1.3 3,297,149 - yhbO b3153 0.29 +3.4 3,297,149 - yhbO b3153 0.29 +0.9 3,297,295 - yhbO b3153 0.58 -0.7 3,297,383 - yhbO b3153 0.75 +2.7 3,297,383 - yhbO b3153 0.75 -1.0 3,297,383 - yhbO b3153 0.75 +1.0 3,297,395 - yhbO b3153 0.77 +0.3 3,297,395 - yhbO b3153 0.77 +0.5 3,297,432 + yhbO b3153 0.84 +0.5 3,297,491 + -1.1 3,297,491 + +2.4 3,297,515 - -0.3 3,297,553 - yhbP b3154 0.13 +1.2 3,297,632 + yhbP b3154 0.31 +2.9 3,297,632 + yhbP b3154 0.31 -2.0 3,297,639 + yhbP b3154 0.33 +0.7 3,297,667 + yhbP b3154 0.39 +1.7 3,297,740 - yhbP b3154 0.55 -1.4 3,297,740 - yhbP b3154 0.55 +4.1 3,297,770 + yhbP b3154 0.62 +1.5 3,297,842 + yhbP b3154 0.78 -0.4 3,297,852 + yhbP b3154 0.81 +1.9 3,297,857 + yhbP b3154 0.82 +0.6
Or see this region's nucleotide sequence