Strain Fitness in Escherichia coli BW25113 around b3130

Experiment: 3-methyl-1-butanol 1.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgarD and sohA are separated by 148 nucleotidessohA and yhaV overlap by 1 nucleotidesyhaV and agaR are separated by 54 nucleotides b3128: garD - (D)-galactarate dehydrogenase (NCBI), at 3,273,304 to 3,274,875 garD b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359 sohA b3130: yhaV - hypothetical protein (NCBI), at 3,275,359 to 3,275,823 yhaV b3131: agaR - DNA-binding transcriptional dual regulator (NCBI), at 3,275,878 to 3,276,687 agaR Position (kb) 3275 3276Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3274.468 kb on - strand, within garDat 3274.508 kb on - strand, within garDat 3274.541 kb on - strand, within garDat 3274.571 kb on + strand, within garDat 3274.619 kb on + strand, within garDat 3274.623 kb on + strand, within garDat 3274.709 kb on + strand, within garDat 3274.836 kb on - strandat 3275.046 kb on + strandat 3275.046 kb on + strandat 3275.049 kb on - strandat 3275.049 kb on - strandat 3275.049 kb on - strandat 3275.051 kb on + strandat 3275.051 kb on + strandat 3275.057 kb on - strandat 3275.059 kb on - strand, within sohAat 3275.063 kb on + strand, within sohAat 3275.098 kb on + strand, within sohAat 3275.108 kb on + strand, within sohAat 3275.108 kb on + strand, within sohAat 3275.118 kb on + strand, within sohAat 3275.118 kb on + strand, within sohAat 3275.318 kb on - strand, within sohAat 3275.318 kb on - strand, within sohAat 3275.392 kb on + strandat 3275.392 kb on + strandat 3275.392 kb on + strandat 3275.400 kb on - strandat 3275.400 kb on - strandat 3275.420 kb on - strand, within yhaVat 3275.427 kb on + strand, within yhaVat 3275.451 kb on + strand, within yhaVat 3275.512 kb on + strand, within yhaVat 3275.536 kb on + strand, within yhaVat 3275.596 kb on + strand, within yhaVat 3275.642 kb on - strand, within yhaVat 3275.642 kb on - strand, within yhaVat 3275.678 kb on + strand, within yhaVat 3275.685 kb on - strand, within yhaVat 3275.685 kb on - strand, within yhaVat 3275.686 kb on - strand, within yhaVat 3275.686 kb on - strand, within yhaVat 3275.690 kb on - strand, within yhaVat 3275.735 kb on + strand, within yhaVat 3275.741 kb on - strand, within yhaVat 3275.781 kb on - strandat 3275.781 kb on - strandat 3276.109 kb on + strand, within agaRat 3276.131 kb on - strand, within agaRat 3276.131 kb on - strand, within agaRat 3276.131 kb on - strand, within agaRat 3276.170 kb on + strand, within agaRat 3276.242 kb on - strand, within agaRat 3276.242 kb on - strandat 3276.270 kb on + strand, within agaRat 3276.447 kb on - strand, within agaRat 3276.447 kb on - strand, within agaRat 3276.560 kb on - strand, within agaRat 3276.602 kb on - strand, within agaRat 3276.625 kb on - strandat 3276.635 kb on - strandat 3276.643 kb on + strandat 3276.762 kb on - strandat 3276.790 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol%
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3,274,468 - garD b3128 0.74 -2.1
3,274,508 - garD b3128 0.77 +2.6
3,274,541 - garD b3128 0.79 +2.1
3,274,571 + garD b3128 0.81 +0.3
3,274,619 + garD b3128 0.84 +1.2
3,274,623 + garD b3128 0.84 -1.0
3,274,709 + garD b3128 0.89 -1.8
3,274,836 - +0.6
3,275,046 + -1.6
3,275,046 + -0.7
3,275,049 - -1.1
3,275,049 - -0.3
3,275,049 - -1.6
3,275,051 + -2.0
3,275,051 + +2.1
3,275,057 - +0.3
3,275,059 - sohA b3129 0.10 -0.7
3,275,063 + sohA b3129 0.12 -1.6
3,275,098 + sohA b3129 0.22 -0.3
3,275,108 + sohA b3129 0.25 -2.4
3,275,108 + sohA b3129 0.25 -0.9
3,275,118 + sohA b3129 0.28 -0.3
3,275,118 + sohA b3129 0.28 +1.4
3,275,318 - sohA b3129 0.88 -2.2
3,275,318 - sohA b3129 0.88 -1.0
3,275,392 + -2.1
3,275,392 + -0.2
3,275,392 + +0.8
3,275,400 - +1.1
3,275,400 - -1.0
3,275,420 - yhaV b3130 0.13 -0.9
3,275,427 + yhaV b3130 0.15 -2.5
3,275,451 + yhaV b3130 0.20 -0.5
3,275,512 + yhaV b3130 0.33 -3.5
3,275,536 + yhaV b3130 0.38 +0.1
3,275,596 + yhaV b3130 0.51 -2.7
3,275,642 - yhaV b3130 0.61 -0.5
3,275,642 - yhaV b3130 0.61 -2.5
3,275,678 + yhaV b3130 0.69 -0.6
3,275,685 - yhaV b3130 0.70 +0.3
3,275,685 - yhaV b3130 0.70 -1.2
3,275,686 - yhaV b3130 0.70 -1.0
3,275,686 - yhaV b3130 0.70 +2.4
3,275,690 - yhaV b3130 0.71 +0.4
3,275,735 + yhaV b3130 0.81 -1.7
3,275,741 - yhaV b3130 0.82 -2.6
3,275,781 - -0.1
3,275,781 - -1.2
3,276,109 + agaR b3131 0.29 -1.3
3,276,131 - agaR b3131 0.31 +1.3
3,276,131 - agaR b3131 0.31 -1.5
3,276,131 - agaR b3131 0.31 -1.5
3,276,170 + agaR b3131 0.36 +0.8
3,276,242 - agaR b3131 0.45 -2.1
3,276,242 - +0.3
3,276,270 + agaR b3131 0.48 +0.3
3,276,447 - agaR b3131 0.70 +0.8
3,276,447 - agaR b3131 0.70 +2.8
3,276,560 - agaR b3131 0.84 -2.1
3,276,602 - agaR b3131 0.89 -2.5
3,276,625 - -0.1
3,276,635 - +1.0
3,276,643 + +0.6
3,276,762 - -2.0
3,276,790 - +0.5

Or see this region's nucleotide sequence