Experiment: 3-methyl-1-butanol 1.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sstT and ygjV are separated by 4 nucleotides ygjV and uxaA are separated by 82 nucleotides
b3089: sstT - sodium:serine/threonine symporter (NCBI), at 3,237,966 to 3,239,210
sstT
b3090: ygjV - conserved inner membrane protein (NCBI), at 3,239,215 to 3,239,766
ygjV
b3091: uxaA - altronate hydrolase (NCBI), at 3,239,849 to 3,241,336
uxaA
Position (kb)
3239
3240 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3238.232 kb on + strand, within sstT at 3238.233 kb on - strand, within sstT at 3238.233 kb on - strand, within sstT at 3238.246 kb on + strand, within sstT at 3238.259 kb on - strand, within sstT at 3238.259 kb on - strand, within sstT at 3238.291 kb on - strand, within sstT at 3238.357 kb on + strand, within sstT at 3238.365 kb on - strand, within sstT at 3238.374 kb on + strand, within sstT at 3238.395 kb on + strand, within sstT at 3238.397 kb on + strand, within sstT at 3238.419 kb on + strand, within sstT at 3238.430 kb on - strand, within sstT at 3238.430 kb on - strand, within sstT at 3238.484 kb on + strand, within sstT at 3238.484 kb on + strand, within sstT at 3238.492 kb on - strand, within sstT at 3238.607 kb on - strand, within sstT at 3238.607 kb on - strand, within sstT at 3238.647 kb on - strand, within sstT at 3238.702 kb on + strand, within sstT at 3238.717 kb on + strand, within sstT at 3238.717 kb on + strand, within sstT at 3238.740 kb on + strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.748 kb on - strand, within sstT at 3238.751 kb on - strand, within sstT at 3238.792 kb on - strand, within sstT at 3238.824 kb on + strand, within sstT at 3238.825 kb on + strand, within sstT at 3238.878 kb on + strand, within sstT at 3238.878 kb on + strand, within sstT at 3238.917 kb on - strand, within sstT at 3238.937 kb on + strand, within sstT at 3238.949 kb on + strand, within sstT at 3239.032 kb on + strand, within sstT at 3239.040 kb on - strand, within sstT at 3239.040 kb on - strand, within sstT at 3239.064 kb on - strand, within sstT at 3239.064 kb on - strand, within sstT at 3239.088 kb on + strand at 3239.100 kb on + strand at 3239.100 kb on + strand at 3239.102 kb on - strand at 3239.102 kb on - strand at 3239.152 kb on - strand at 3239.152 kb on - strand at 3239.348 kb on + strand, within ygjV at 3239.360 kb on + strand, within ygjV at 3239.368 kb on + strand, within ygjV at 3239.400 kb on + strand, within ygjV at 3239.400 kb on + strand, within ygjV at 3239.424 kb on - strand, within ygjV at 3239.487 kb on + strand, within ygjV at 3239.526 kb on - strand, within ygjV at 3239.544 kb on + strand, within ygjV at 3239.544 kb on + strand, within ygjV at 3239.552 kb on - strand, within ygjV at 3239.585 kb on + strand, within ygjV at 3239.585 kb on + strand, within ygjV at 3239.653 kb on + strand, within ygjV at 3239.741 kb on + strand at 3239.741 kb on + strand at 3239.807 kb on + strand at 3239.899 kb on - strand at 3239.899 kb on - strand at 3239.937 kb on - strand at 3239.973 kb on + strand at 3239.986 kb on - strand at 3240.082 kb on - strand, within uxaA at 3240.094 kb on - strand, within uxaA at 3240.094 kb on - strand, within uxaA at 3240.096 kb on + strand, within uxaA at 3240.096 kb on + strand, within uxaA at 3240.099 kb on - strand, within uxaA at 3240.106 kb on + strand, within uxaA at 3240.114 kb on - strand, within uxaA at 3240.128 kb on + strand, within uxaA at 3240.166 kb on + strand, within uxaA at 3240.166 kb on + strand, within uxaA at 3240.176 kb on + strand, within uxaA at 3240.176 kb on + strand, within uxaA at 3240.255 kb on - strand, within uxaA at 3240.262 kb on - strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.277 kb on + strand, within uxaA at 3240.281 kb on + strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.347 kb on - strand, within uxaA at 3240.359 kb on + strand, within uxaA at 3240.359 kb on + strand, within uxaA at 3240.415 kb on - strand, within uxaA at 3240.415 kb on - strand, within uxaA at 3240.454 kb on + strand, within uxaA at 3240.454 kb on + strand, within uxaA at 3240.470 kb on - strand, within uxaA at 3240.556 kb on - strand, within uxaA at 3240.614 kb on - strand, within uxaA at 3240.661 kb on - strand, within uxaA at 3240.661 kb on - strand, within uxaA at 3240.743 kb on + strand, within uxaA at 3240.743 kb on + strand, within uxaA
Per-strain Table
Position Strand Gene LocusTag Fraction 3-methyl-1-butanol 1.25 vol% remove 3,238,232 + sstT b3089 0.21 +0.3 3,238,233 - sstT b3089 0.21 -1.9 3,238,233 - sstT b3089 0.21 +0.5 3,238,246 + sstT b3089 0.22 +0.3 3,238,259 - sstT b3089 0.24 -1.9 3,238,259 - sstT b3089 0.24 +1.2 3,238,291 - sstT b3089 0.26 +0.2 3,238,357 + sstT b3089 0.31 -0.5 3,238,365 - sstT b3089 0.32 -1.2 3,238,374 + sstT b3089 0.33 -1.6 3,238,395 + sstT b3089 0.34 +0.9 3,238,397 + sstT b3089 0.35 -0.1 3,238,419 + sstT b3089 0.36 +1.3 3,238,430 - sstT b3089 0.37 -0.8 3,238,430 - sstT b3089 0.37 +0.7 3,238,484 + sstT b3089 0.42 -1.3 3,238,484 + sstT b3089 0.42 -1.3 3,238,492 - sstT b3089 0.42 +0.3 3,238,607 - sstT b3089 0.51 +1.8 3,238,607 - sstT b3089 0.51 +2.8 3,238,647 - sstT b3089 0.55 +0.4 3,238,702 + sstT b3089 0.59 +0.3 3,238,717 + sstT b3089 0.60 -2.4 3,238,717 + sstT b3089 0.60 -2.2 3,238,740 + sstT b3089 0.62 -1.9 3,238,748 - sstT b3089 0.63 -2.0 3,238,748 - sstT b3089 0.63 -0.7 3,238,748 - sstT b3089 0.63 -0.3 3,238,751 - sstT b3089 0.63 -0.7 3,238,792 - sstT b3089 0.66 +1.3 3,238,824 + sstT b3089 0.69 +0.6 3,238,825 + sstT b3089 0.69 -1.0 3,238,878 + sstT b3089 0.73 -1.3 3,238,878 + sstT b3089 0.73 +0.9 3,238,917 - sstT b3089 0.76 +0.0 3,238,937 + sstT b3089 0.78 +0.1 3,238,949 + sstT b3089 0.79 +0.1 3,239,032 + sstT b3089 0.86 +1.0 3,239,040 - sstT b3089 0.86 -0.3 3,239,040 - sstT b3089 0.86 +0.4 3,239,064 - sstT b3089 0.88 -1.5 3,239,064 - sstT b3089 0.88 -0.1 3,239,088 + +0.1 3,239,100 + -2.5 3,239,100 + +0.0 3,239,102 - +2.3 3,239,102 - -0.0 3,239,152 - +1.2 3,239,152 - +2.1 3,239,348 + ygjV b3090 0.24 -0.3 3,239,360 + ygjV b3090 0.26 +1.3 3,239,368 + ygjV b3090 0.28 +1.3 3,239,400 + ygjV b3090 0.34 -0.3 3,239,400 + ygjV b3090 0.34 +2.2 3,239,424 - ygjV b3090 0.38 -0.8 3,239,487 + ygjV b3090 0.49 -1.6 3,239,526 - ygjV b3090 0.56 +2.0 3,239,544 + ygjV b3090 0.60 +0.6 3,239,544 + ygjV b3090 0.60 +1.4 3,239,552 - ygjV b3090 0.61 +0.0 3,239,585 + ygjV b3090 0.67 +0.5 3,239,585 + ygjV b3090 0.67 +1.1 3,239,653 + ygjV b3090 0.79 +0.4 3,239,741 + +1.0 3,239,741 + +0.3 3,239,807 + +0.6 3,239,899 - +0.1 3,239,899 - +1.5 3,239,937 - +0.3 3,239,973 + +1.8 3,239,986 - -0.5 3,240,082 - uxaA b3091 0.16 -2.1 3,240,094 - uxaA b3091 0.16 +0.2 3,240,094 - uxaA b3091 0.16 -0.7 3,240,096 + uxaA b3091 0.17 -1.4 3,240,096 + uxaA b3091 0.17 -0.8 3,240,099 - uxaA b3091 0.17 -1.6 3,240,106 + uxaA b3091 0.17 -0.5 3,240,114 - uxaA b3091 0.18 -0.2 3,240,128 + uxaA b3091 0.19 -0.3 3,240,166 + uxaA b3091 0.21 +0.4 3,240,166 + uxaA b3091 0.21 -1.6 3,240,176 + uxaA b3091 0.22 -0.4 3,240,176 + uxaA b3091 0.22 +0.7 3,240,255 - uxaA b3091 0.27 -1.0 3,240,262 - uxaA b3091 0.28 -0.3 3,240,277 + uxaA b3091 0.29 -2.5 3,240,277 + uxaA b3091 0.29 +0.6 3,240,277 + uxaA b3091 0.29 +0.3 3,240,281 + uxaA b3091 0.29 +0.9 3,240,347 - uxaA b3091 0.33 -0.5 3,240,347 - uxaA b3091 0.33 +1.4 3,240,347 - uxaA b3091 0.33 +0.6 3,240,359 + uxaA b3091 0.34 -0.4 3,240,359 + uxaA b3091 0.34 +0.7 3,240,415 - uxaA b3091 0.38 +2.1 3,240,415 - uxaA b3091 0.38 +1.0 3,240,454 + uxaA b3091 0.41 -0.6 3,240,454 + uxaA b3091 0.41 -0.2 3,240,470 - uxaA b3091 0.42 +0.4 3,240,556 - uxaA b3091 0.48 -0.8 3,240,614 - uxaA b3091 0.51 -0.6 3,240,661 - uxaA b3091 0.55 -3.2 3,240,661 - uxaA b3091 0.55 +3.8 3,240,743 + uxaA b3091 0.60 +0.7 3,240,743 + uxaA b3091 0.60 +1.7
Or see this region's nucleotide sequence